Skip to main content

Purification of a non-histone protein with properties of antizyme to ornithine decarboxylase from germinated barley seeds

  • Chapter
Polyamines in Plants

Part of the book series: Advances in Agricultural Biotechnology ((AABI,volume 18))

  • 63 Accesses

Abstract

The purification of a chromatin-bound antizyme to ornithine decarboxylase from germinated barley seeds is described. This antizyme was extracted from chromatin by 2M NaCl and purified to homogeneity. Its molecular weight was found to be 9000 with an isoelectric point of 4.1. It reacts with both cytosolic and chromatin- bound ornithine decarboxylase from generated barley seeds and E. coli but it does not inhibit ornithine decarboxylase of Tetrahymena pyriformis or rat liver.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 169.00
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 219.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 219.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Bonner J. Chalkley C.R. Dahmus M. Fambrough D. Fujimura F. Huang RC, Huberman J. Jenson R. Marushige K. Ohlenbusch H. Olivera B, Widholm J (1968) Isolation and characterization of chromosomal nucleoproteins.ln: Grossman L, Moldaave K.eds. Methods in Enzymology l2B,pp3–65. New York Academic Press

    Google Scholar 

  2. Brosnan ME. Farrell R. Wilansky H, Williamson DH (1983) Effect of starvation and refeeding on polyamine concentrations and ornithine decarboxylase antizyme in mammary glands of lactating rats. Biochem J 212: 149–153

    PubMed  CAS  Google Scholar 

  3. Canellakis KS, Viceps-Madore D, Kyriakidis DA, Heller JS (1979) The regulation and function of ornithine decarboxylase and of the polyamines. In: Horecker B, Stadtman E. eds. Current Topics in Cellular Regulation 15, pp 155–202. New York: Academic Press

    Google Scholar 

  4. Canellakis FS. Heller JS, Kyriakidis DA (1981) The interaction of ornithine decarboxylase with its antizyme. In: Caldarera CM. Zappia V, Bachrach U, eds. Advances in Polyamine Research 3.pp 1–13. New York: Raven Press

    Google Scholar 

  5. Champoux JJ (1978) Proteins that affect DNA conformation. Ann Rev Biochem 47: 449–479

    Article  PubMed  CAS  Google Scholar 

  6. Fong WF. Heller JS, Canellakis ES (1976) The appearance of an ornithine decarboxylase inhibitory protein upon addition of putrescine to cell cultures. Biochim Biophys Acta 428: 456–465

    PubMed  CAS  Google Scholar 

  7. Fujita K, Matsufuji S. Murakami Y, Hayashi S (1984) Antizyme to ornithine decarboxylase is present in the liver of starved rats. Blocbem J 218: 557–562

    CAS  Google Scholar 

  8. Keller JS. Fong WF, Canellakis FS (1976) Induction of a protein inhibitor to ornithine decarboxylase by the end product of its reaction. Proc Natl Acad Sci (USA) 73: 1858–1862

    Article  Google Scholar 

  9. Heller JS, Kyriakidis DA, Fong WF, Canellakis ES (1977) Ornithine decarboxylase antizyme is a normal component of uninduced H-35 cells and rat liver. Eur J Biochem 81: 545–550

    Article  PubMed  CAS  Google Scholar 

  10. Heller JS, Canellakis ES (1981) Cellular control of ornithine decarboxylase activity by its antizyme. J Cell Physiol 107: 209–217

    Article  PubMed  CAS  Google Scholar 

  11. Helfer JS. Kyriakidis DA, Canellakis ES (1983) Purification and properties of the antizymes of Escherichia coli to ornithine decarboxylase. Biochim Biophys Acta 760: 154–162

    Google Scholar 

  12. Helfer JS. Rostomily R. Kyrtakidis DA, Canellakis ES (1983) Regulation or polyamine biosynthesis in Escherichia coli by basic proteins. Proc Natl Acad Sci (USA) 80: 5181–5184

    Article  Google Scholar 

  13. Huang SC. Kyriakidis DA. Rinehart CA, Canellakis ES (1984) Reversal of the antizyme inhibition of ornithine decarboxylase by nucleic acids. Biochem Pharm 33: 1383–1386

    Article  PubMed  CAS  Google Scholar 

  14. Kowalczykowski SC. Bear DG, Von Hippel PH (1981) Single-stranded DNA binding proteins. In: Boyer PD, ed. lite Enzymes 14. pp 373–444. New York: Academic Press

    Google Scholar 

  15. Kyriawkidis DA. Heller JS, Canellakis ES (1978) Modulation of ornithine decarboxylase activity in Escherichia coli by positive and negative effectors. Proc Natl Acad Sci (USA) 75: 4699–4703

    Article  Google Scholar 

  16. Kyriakidis DA (1983) Macromolecular effectors of ornithine decarboxylase activity in germinating barley seeds. In: Bachrach U. Kaye A, Chayen R. eds. Advances in Polyamine Research 4, pp 427–436. New York: Raven Press

    Google Scholar 

  17. Kyriakidis DA, Heller JS, Canellakis ES (1983) Purification of ornithine decarboxylase antizymes (Escherichia coli). In Tabor H, Tabor CW, eds. Methods in Enzymology 94. pp 193–199. New York: Academic Press

    Google Scholar 

  18. Kyriakidis DA. Panagiotidis CA, Georgatsos JG (1983) Ornithine decarboxylase (germinated barley seeds). In: Tabor H, Tabor CW, eds. Methods in Enzymology 94.pp 162–166. New York: Academic Press

    Google Scholar 

  19. Kyriakidis DA (1983) Effect of plant growth hormones and polyamines on ornithine decarboxylase activity during the germination of barley seeds. Physiol Plant 57 499–504

    Article  CAS  Google Scholar 

  20. Kyriakidis DA, Flamigni F, Pawlak JW, Canellakis ES (1984) Mode of interaction of ornithine decarboxylase with antizyme and α-difluoromethylornithine. Biochem Pharm 33: 1575–1578

    Article  PubMed  CAS  Google Scholar 

  21. Laemmli UK (1970)Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680–685

    Article  PubMed  CAS  Google Scholar 

  22. Panagiotidis CA. Georgatsos JG, Kyriakidis DA (1982) Superinduction of cytosolic and chromatin-bound ornithine decarboxylase activities of germinating barley seeds by actinomycin D. FEBS Lett 146: 193–196

    Article  CAS  Google Scholar 

  23. Takada Y, Ohlendorf DH. Anderson WF, Matthews BW (1983) DNA-binding proteins. Science 221: 1020–1026

    Article  Google Scholar 

  24. Wray W. Boulikas T. Wray VP, Hancock R (1981) Silver staining of proteins in polyacrylamide gels Anal Biochem 118: 197–203

    Article  PubMed  CAS  Google Scholar 

  25. Wringley CW (1971) Gel electrophoresis. In: Jakoby WB,ed. Methods in Enzymology 22. pp 559–564. New York: Academic Press

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 1985 Martinus Nijhoff/Dr W. Junk Publishers, Dordrecht

About this chapter

Cite this chapter

Panagiotidis, C.A., Kyriakidis, D.A. (1985). Purification of a non-histone protein with properties of antizyme to ornithine decarboxylase from germinated barley seeds. In: Galston, A.W., Smith, T.A. (eds) Polyamines in Plants. Advances in Agricultural Biotechnology, vol 18. Springer, Dordrecht. https://doi.org/10.1007/978-94-009-5171-6_4

Download citation

  • DOI: https://doi.org/10.1007/978-94-009-5171-6_4

  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-94-010-8788-9

  • Online ISBN: 978-94-009-5171-6

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics