Abstract
Chromatin prepared from maize shoot tips using as extraction medium including quinacrine as an. inhibitor of polyamine oxidate, contained 1.6pmol spermidine μg DNA-1 and 14.8pmol spermine μg DNA-1, respectively. This represented 0.1% spermidine and 3.7% spermine as compared with the content of those amines in the whote tissue. No putrescine was detectable in the chromatin preparation When contamination of polyamines in the preparation was determined by the addition of labeled polyamines to the extraction medium, the ratio of the polyamines in the preparation to those in the extraction medium was 0.1% spermidine and 0.7% spermine, respectively. Spermine In the chromatin preparation almost fully solubilized by a DNase-treatment, but spermidine was less easily solubilizcd. Most of the spermine associated with the chromation is chromatin-specific.
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© 1985 Martinus Nijhoff/Dr W. Junk Publishers, Dordrecht
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Hirasawa, E., Suzuki, Y. (1985). Occurrence of spermine in chromatin of Zea mays . In: Galston, A.W., Smith, T.A. (eds) Polyamines in Plants. Advances in Agricultural Biotechnology, vol 18. Springer, Dordrecht. https://doi.org/10.1007/978-94-009-5171-6_3
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DOI: https://doi.org/10.1007/978-94-009-5171-6_3
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