Summary
In vitro growth (total protein content) of 5 Frankia isolates was significantly inhibited at 10-4 M juglone (5-hydroxy-l ,4-napthoquinone) concentration, but the degree of inhibition varied with the Frankia isolate. Isolates from Alnus crispa [Alnus viridis ssp. crispa (Ait.) Turril] were most tolerant of 10-4 M juglone relative to controls, while an isolate from Purshia tridentata (Pursh.) D.C. was most inhibited, displaying a dramatic decrease in growth and greatly altered morphology.
Nodulation of black alder [Alnus glutinosa L. (Gaertn.)] in an amended prairie soil inoculated with a Frankia isolate from red alder (Alnus rubra Bong.) was significantly decreased by the addition of aqueous suspensions of 10-3 M and 10-4 M juglone. This decrease was partially independent of decreased plant growth. The addition of an equal volume of sand to the soil mixture further decreased nodulation of black alder. Frankia inoculation of the soil mixtures significantly increased the total number of nodules formed per seedling, and the degree of differences in seedling nodulation owing to juglone and soil treatments.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Alexander M 1977 Introduction to Soil Microbiology. John Wiley and Sons, Inc., NY, 467 p.
Berry A and Torrey J G 1979 Isolation and characterization in vivo and in vitro of an actinomycetous endophyte from Alnus rubra Bong. In Symbiotic Nitrogen Fixation in the Management of Temperate Forests. Eds. J C Gordon, C T Wheeler and D A Perry. Oregon State University, Corvallis, OR, pp 69–83.
Bradford M M 1976 A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principal of protein-dye binding. Anal. Biochem. 72, 248–254.
Bryant J P, Wieland G D, Reichardt P B, Lewis V E and McCarthy M C 1983 Pinosylvin methyl ether deters snowshoe hare feeding on green alder. Science 222, 1023–1025.
Callaham D, Del Tredici P and Torrey J G 1978 Isolation and cultivation in vitro of the actinomycete causing root nodulation in Comptonia. Science 199, 899–902.
Coder K D 1983 Seasonal changes of juglone potential in leaves of black walnut (Juglans nigra L.). J. Chem. Ecol. 9, 1203–1212.
Daglish C 1950 The isolation and identification of a hydrojuglone glucoside occurring in the walnut. Biochem. J. 47, 452–457.
Dawson J O and Seymour P E 1983 Effects of juglone concentration on growth in vitro of Frankia ArI3 and Rhizobium japonicum strain 71. J. Chem. Ecol. 9, 1175–1183.
Fisher R F 1978 Juglone inhibits pine growth under certain moisture regimes. Soil Sci. Soc. Am. J. 42, 801–803.
Funk D T, Schlesinger R C and Ponder F 1979 Autumn-olive as a nurse plant for black walnut. Bot. Gaz. 140 (Suppl.), 110–114.
Granato T C, Banwart W L, Porter P M and Hassett J J 1983 Effect of variety and stage of growth on potential allelochemical compounds in soybean roots. J. Chem. Ecol. 9, 1281–1292.
Hedin P A, Langhans V E and Graves C H Jr 1979 Identification of juglone in pecan as a possible factor of resistance to Fusicladium effusum. J. Agric. Food. Chem. 27, 92–94.
Hedin P A, Collum D H, Langhans V E and Graves C H 1980 Distribution of juglone and related compounds in pecan and their effects on Fusicladium effusum. J. Agric. Food Chem. 28, 340–342.
Helwig J T (ed.) SAS user’s Guide, 1979 edition. SAS Institute, Inc., Cary, NC, 495 p.
Jobidon R and Thibault J R 1982 Allelopathic growth inihibtion of nodulated and un- nodulated Alnus crispa seedlings by Populus balsamifera. Am. J. Bot. 69, 1213–1223.
Koeppe D E 1972 Some reactions of isolated corn mitochondria influenced by juglone. Physiol. Plant, 27, 89–94.
Lalonde M and Calvert H E 1979 Production of Frankia hyphae and spores as an infective inoculant for Alnus species. In Symbiotic Nitrogen Fixation in the Management of Temperate Forests. Eds. J C Gordon, C T Wheeler and D A Perry. Oregon State University, Corvallis, OR, pp 95–110.
Li C Y 1974 Phenolic compounds in understory species of alder, conifer, and mixed alder-conifer stands of coastal Oregon. Lloydia 37, 603–607.
Perradin Y, Mottet M J and Lalonde M 1983 Influence of phenolics on in vitro growth of Frankia strains. Can. J. Bot. 61, 2807–2814.
Rietveld W J 1983 Allelopathic effects of juglone on germination and growth of several herbaceous and woody species. J. Chem. Ecol. 9, 295–308.
Rietveld W J, Schlesinger R C and Kessler K J 1983 Allelopathic effects of black walnut on European black alder coplanted as a nurse species. J. Chem. Ecol. 9, 1119–1133.
Torrey J G 1978 Nitrogen fixation by actinomycete-nodulated angiosperms. Bioscience 28, 586–592.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1985 Martinus Nijhoff Publishers, Dordrecht
About this chapter
Cite this chapter
Vogel, C.S., Dawson, J.O. (1985). Effect of juglone on growth in vitro of Frankia isolates and nodulation of Alnus glutinosa in soil. In: Lalonde, M., Camiré, C., Dawson, J.O. (eds) Frankia and Actinorhizal Plants. Developments in Plant and Soil Sciences, vol 18. Springer, Dordrecht. https://doi.org/10.1007/978-94-009-5147-1_8
Download citation
DOI: https://doi.org/10.1007/978-94-009-5147-1_8
Publisher Name: Springer, Dordrecht
Print ISBN: 978-94-010-8777-3
Online ISBN: 978-94-009-5147-1
eBook Packages: Springer Book Archive