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Characterization and infectivity of a spontaneous variant isolated from Frankia sp. WEY 0131391

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Frankia and Actinorhizal Plants

Part of the book series: Developments in Plant and Soil Sciences ((DPSS,volume 18))

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Summary

A spontaneous variant, obtained from a Frankia isolate from Alnus rubra nodules, was compared with the parent strain with regard to infeetivity, nitrogenase activity, and electro- phoretic and immunological profiles. Both the parent and the variant strain were equally effective in inducing nodulation in seedlings of A. rubra. All inoculated plants had an active nitrogenase system as measured by the acetylene reduction assay. Electrophoresis of whole cell homogenates on SDS-polyacrylamide slab gels showed similar electrophoretic profiles; however, the variant strain also exhibited striking differences in protein patterns that distinguish it from the parent strain. Immunological analysis of the original Frankia strain and its variant revealed shared antigens as well as immunologically distinct antigenic determinants in the two strains. The variant strain exhibits a distinct morphology and growth patterns which remain stable after many passages through culture.

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Author information

Authors and Affiliations

  1. Department of Biotechnology, Weyerhaeuser Technology Center, Tacoma, WA, 98477, USA

    Willie L. Parson, Linda R. Robertson & Carolyn V. Carpenter

  2. The Evergreen State College, Olympia, WA, 98505, USA

    Willie L. Parson

Authors
  1. Willie L. Parson
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  2. Linda R. Robertson
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  3. Carolyn V. Carpenter
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Editor information

Editors and Affiliations

  1. Département des sciences forestières Faculté de foresterie, Université Laval, Ste-Foy, Qué., G1K 7P4, Canada

    M. Lalonde  & C. Camiré  & 

  2. Forestry Department, University of Illinois, 61801, Urbana, IL, USA

    J. O. Dawson

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© 1985 Martinus Nijhoff Publishers, Dordrecht

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Parson, W.L., Robertson, L.R., Carpenter, C.V. (1985). Characterization and infectivity of a spontaneous variant isolated from Frankia sp. WEY 0131391. In: Lalonde, M., Camiré, C., Dawson, J.O. (eds) Frankia and Actinorhizal Plants. Developments in Plant and Soil Sciences, vol 18. Springer, Dordrecht. https://doi.org/10.1007/978-94-009-5147-1_3

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  • DOI: https://doi.org/10.1007/978-94-009-5147-1_3

  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-94-010-8777-3

  • Online ISBN: 978-94-009-5147-1

  • eBook Packages: Springer Book Archive

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