Abstract
Populations of peritoneal macrophages are heterogeneous. Not only in acute or chronical inflammatory reactions but also in the steady state they comprise macrophages with a different pattern of endogenous peroxidatic activity on the ultrastructural level. The relevance of this pattern to the detection of different maturation steps was extensively discussed at the Third Conference on Mononuclear. Phagocytes (1, 2). Beelen, et al. (3) demonstrated the exudate-resident macrophage as a transitional cell in vivo in the rat and in vitro in the mouse, rat and man. A low but consistent number of peroxidase negative macrophages was also present and these cells could represent a transitional form too. De Bakker (4), however, was not able to demonstrate exudate-resident macrophages in the steady state and in the early stages of an acute inflammatory reaction in the mouse. She suggested that resident macrophages develop from the negative cells only. In this contribution we quantified the different types of macrophage in the steady state and during an acute peritonitis induced with newborn calf serum (NBCS). Moreover, evidence from recent experiments (5) is summarized for the relation between the development of resident pattern of endogenous peroxidatic activity and the expression of F4/80 antigen. This antigen is specific for mouse macrophages and is not expressed on circulating monocytes (6).
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Hoefsmit, E.C.M., Beelen, R.H.J. (1985). The expression of antigen F4/80 and la on peritoneal macrophages in normal and BCG immunized mice. In: van Furth, R. (eds) Mononuclear Phagocytes. Springer, Dordrecht. https://doi.org/10.1007/978-94-009-5020-7_3
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DOI: https://doi.org/10.1007/978-94-009-5020-7_3
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