Abstract
In the past decade recombinant DNA technology has expanded out from the academic environment and into industrial and clinical laboratories around the world. Vaccines and diagnostic kits based on recombinant DNA-derived products are now available with many more practical applications soon to follow. For such products to be used effectively it is important that the potential and the limitations of the new technologies are widely understood by the whole range of specialists working in laboratory medicine. A review of the principal techniques of genetic engineering from cloning and characterizing genes to the expression of foreign antigens inEscherichia coli, yeast and mammalian cells will be presented. Some of the ways in which these new approaches will have a major impact on diagnosis and treatment of infections and inherited metabolic disorders will be discussed.
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© 1985 MTP Press Limited
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Winther, M.D. (1985). Theoretical and practical aspects of recombinant DNA techniques relevant to the clinical sciences. In: Shinton, N.K. (eds) New Technologies in Clinical Laboratory Science. Springer, Dordrecht. https://doi.org/10.1007/978-94-009-4928-7_5
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DOI: https://doi.org/10.1007/978-94-009-4928-7_5
Publisher Name: Springer, Dordrecht
Print ISBN: 978-94-010-8684-4
Online ISBN: 978-94-009-4928-7
eBook Packages: Springer Book Archive