Skip to main content

Cryopreservation of Woody Species

  • Chapter
Book cover Cell and Tissue Culture in Forestry

Part of the book series: Forestry Sciences ((FOSC,volume 24-26))

Abstract

The irretrievable loss of the naturally occurring germplasm is a problem of great concern to tree breeders. Many countries are facing serious gene pool losses in populations of some valuable tree species (4). With increasing world demand for forest products and continuing deforestation, the conservation of genetic resources has become an urgent issue. The preservation of available germplasm for use in future tree improvement is imperative.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 169.00
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 219.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 219.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. BAJAJ YPS 1983 Production of normal seeds from plants regenerated from the meristems of Arachis hypogaea and Cicer arietinum cryopreserved for 20 months. Euphytica 32: 425–430

    Article  Google Scholar 

  2. BINDER WD, B ZAERR 1980 Freeze preservation of suspension cultured cells of a gymnosperm, Douglas-fir. Cryobiology 17:624 (Abst)

    Google Scholar 

  3. BINDER WD, B ZAERR 1980 Freeze preservation of suspension cultured cells of a hardwood, poplar. Cryobiology 17: 624–625 (Abst)

    Google Scholar 

  4. BONGA JM 1977 Application of tissue culture in forestry. In J Reinert, YPS Bajaj, eds, Applied and Fundamental Aspects of Plant Cell, Tissue, and Organ Culture. Springer-Verlag, Berlin, pp 93–108

    Google Scholar 

  5. CHEN, THH, KK KARTHA, NL LEUNG, GWG KURZ, KB CHATSON, F CONSTABEL 1984 Cryopreservation of alkaloid-producing cell cultures of periwinkle (Catharanthus roseus). Plant Physiol 75: 726–731

    Article  PubMed  CAS  Google Scholar 

  6. FINKLE BJ, J ULRICH 1979 Effects of cryoprotectants in combination on the survival of frozen sugarcane cells. Plant Physiol 63: 598–604

    Article  PubMed  CAS  Google Scholar 

  7. FINKLE BJ, J ULRICH 1982 Cryoprotectant removal as a factor in the survival of frozen rice and sugarcane cells. Cryobiology 19: 329–335

    Article  PubMed  CAS  Google Scholar 

  8. FINKLE BJ, ME ZAVALA, J. ULRICH 1985 Cryoprotective compounds in the viable freezing of plant tissues. In KK Kartha, ed, Cryopreservation of Plant Cells and Organs, CRC Press, pp 75–113

    Google Scholar 

  9. GROUT BWW, GG HENSHAW 1978 Freeze preservation of potato shoot-tip cultures. Ann Bot 42: 1227–1229

    Google Scholar 

  10. KARTHA KK 1982 Cryopreservation of germplasm using meristem and tissue culture. n DT Tomes, BE Ellis, PM Harney, KJ Kasha and RL Peterson, eds, Application of Plant Cell and Tissue Culture to Agriculture and Industry, Univ Guelph, Guelph, Ontario, pp 136–161

    Google Scholar 

  11. KARTHA KK 1985 Meristem culture and germplasm preservation. In KK Kartha, ed, Cryopreservation of Plant Cells and Organs, CRC Press, pp 115–134

    Google Scholar 

  12. KARTHA KK, NL LEUNG, OL GAHBORG 1979 Freeze-preservation of pea meristems in liquid nitrogen and subsequent plant regeneration. Plant Sci Lett 15:7–15

    Article  Google Scholar 

  13. KARTHA KK, NL LEUNG, K. PAHL 1980 Cryopreservation of strawberry meristems and mass propagation of plantlets. J Am Soc Hortic Sci 105:481–484

    CAS  Google Scholar 

  14. KARTHA KK, NL LEUNG, P GAUDET-LAPRAIRIE, F CONSTABEL 1982 Cryopreservation of periwinkle Catharanthus roseus cells cultured in vitro. Plant Cell Rep 1: 135–138

    Article  Google Scholar 

  15. KARTHA KK, NL LEUNG, LA MROGINSKI 1982 In vitro growth responses and plant regeneration from cryopreserved meristems of cassava (Manihot esculenta Crantz). Z Pflanzenphysiol 107:133–140

    Google Scholar 

  16. KATANO M, A ISHIHARA, A SAKAI 1983 Survival of dormant apple shoot tips after immersion in liquid nitrogen. HortScience 18:707–708

    Google Scholar 

  17. LARKIN PJ, WR SCOWCROFT 1981 Somaclonal variation — a noval source of variability from cell cultures. Theor Appl Genet 60: 197–214

    Article  Google Scholar 

  18. MADDOX AD, FG GONSALVES, R. SHIELDS 1983 Successful cryopreservation of suspension cultures of three Nicotiana species at the temperature of liquid nitrogen. Plant Sci Lett 28:157–162

    Google Scholar 

  19. MAZUR P 1970 Cryobiology: The freezing of biological systems. Science 168:939–949

    Article  PubMed  CAS  Google Scholar 

  20. POWLEDGE TM 1984 Biotechnology touches the forest. Biotechnology 2:763–772

    Article  Google Scholar 

  21. NAG KK, HE STREET 1975 Freeze-preservation of cultured plant cells. I. The pretreatment phase. Physiol Plant 34:254–260

    Article  Google Scholar 

  22. SAKAI A, Y SUGAWARA 1973 Survival of poplar callus at super-low temperatures after cold acclimation. Plant Cell Physiol 14: 1201–1204

    Google Scholar 

  23. SAKAI A, Y NISHIYAMA 1978 Cryopreservation of winter vegetative buds of hardy fruit trees in liquid nitrogen. HortScience 13:225–227

    Google Scholar 

  24. SALA F, R CELLA, F ROLLO 1979 Freeze-preservation of rice cells. Physiol Plant 45:170–176

    Article  CAS  Google Scholar 

  25. SEIBERT M 1976 Shoot initiation from carnation shoot apices frozen to-196°C. Science 191:1178–1179

    Article  PubMed  CAS  Google Scholar 

  26. SEIBERT M, PG WETHERBEE 1977 Increased survival and differentiation of frozen herbaceous plant organ cultures through cold treatment. Plant Physiol 59: 1043–1046

    Article  PubMed  CAS  Google Scholar 

  27. STANWOOD PC 1985 Cryopreservation of seed germplasm for genetic conservation. In KK Kartha, ed, Cryopreservation of Plant Cells and Organs, CRC Press, pp 200–226

    Google Scholar 

  28. SUGAWARA Y, A SAKAI 1974 Survival of suspension cultured sycamore cells cooled to the temperature of liquid nitrogen. Plant Physiol 52:722–724

    Article  Google Scholar 

  29. THORPE. TA, S BIONDI 1984 Conifers. In. WR Sharp, DA Evans, PV Ammirato, Y Yamada, eds, Handbook of Plant Cell Culture, Vol 2, Macmillian Pub Co, pp 435–470

    Google Scholar 

  30. TOWILL LE, P MAZUR 1974 Effects of cooling and warming rates on survival of frozen Acer saccarum tissue cultures. Plant Physiol 54 (Suppl):10

    Google Scholar 

  31. ULRICH JM, BJ FINKLE, PH MOORE, H GINOZA 1979 Effect of a mixture of cryoprotectants in attaining liquid nitrogen survival of callus cultures of a tropical plant. Cryobiology 16:550–556

    Article  PubMed  CAS  Google Scholar 

  32. ULRICH JM, RA MICKLER, BJ FINKLE, DF KARNOSKY 1984 Survival and regeneration of American elm callus cultures after being frozen in liquid nitrogen. Can J For Res 14:750–753

    Article  Google Scholar 

  33. WEBER G, EJ ROTH, HG SCHWEIGER 1983 Storage of cell suspensions and protoplasts of Glycine max (L.) Merr, Brassica napus (L.), Datura innoxia (Mill.) and Daucus carota (L.) by freezing. Z Pflanzenphysiol 109: 29–39

    Google Scholar 

  34. WITHERS LA 1978 The freeze-preservation of synchronously dividing cultured cells of Acer pseudoplatanus. Cryobiology 15:87–92

    Article  PubMed  CAS  Google Scholar 

  35. WITHERS LA 1980 Tissue Culture Storage for Genetic Conservation. Tech Report, Int Board Plant Genetic Resources, FAO, United Nations, Rome

    Google Scholar 

  36. WITHERS LA 1982 The development of cryopreservation techniques for plant cell, tissue and organ cultures. In Fujiwara, ed, Proc Fifth Int Congr Plant Tissue Culture, Tokyo, pp 793–794

    Google Scholar 

  37. WITHERS LA, PJ KING 1979 Proline-A novel cryoprotectant for the freeze-preservation of Zea mays. Plant Physiol 64:675–678

    Article  PubMed  CAS  Google Scholar 

  38. WITHERS LA, PJ KING 1980 A simple freezing unit and routine cryopreservation method for plant cell cultures. Cryo Lett 1:213–220

    Google Scholar 

  39. WITHERS LA 1985 Cryopreservation of cultured plant cells and protoplasts, In KK Kartha, ed, Cryopreservation of Plant Cells and Organs. CRC Press, pp 243–267

    Google Scholar 

  40. WITHERS LA, HE STREET 1977 The Freeze-preservation of cultured plant cells: III The pregrowth phase, Physiol Plant 39:171–178

    Article  Google Scholar 

Download references

Authors

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 1987 Martinus Nijhoff Publishers, Dordrecht

About this chapter

Cite this chapter

Chen, T.H.H., Kartha, K.K. (1987). Cryopreservation of Woody Species. In: Bonga, J.M., Durzan, D.J. (eds) Cell and Tissue Culture in Forestry. Forestry Sciences, vol 24-26. Springer, Dordrecht. https://doi.org/10.1007/978-94-009-4484-8_16

Download citation

  • DOI: https://doi.org/10.1007/978-94-009-4484-8_16

  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-94-010-8497-0

  • Online ISBN: 978-94-009-4484-8

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics