Abstract
We reported previously (1) that mutants (Exo-) of Rhizobium meliloti SU47 which failed to secrete the acidic extra-cellular polysaccharide formed abnormal nodules on Medicago sativa that did not fix nitrogen and did not contain bacteroids. This was true of mutants belonging to six different genetic complementation groups, and established a strong correlation between the ability to secrete the polysaccharide and the ability to invade nodules. These mutants were isolated on the basis of their non-fluorescent colony phenotype on agar medium containing the fluorescent stain calcofluor. Three of the loci defined by these mutants (ExoA, ExoB, and ExoF) were shown to reside in a cluster on the megaplasmid pRmeSU47b (2).
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Similar content being viewed by others
References
Leigh, JA, Signer, ER, and Walker, GC (1985) Exopolysaccharide-deficient mutants of Rhizobium meliloti that form ineffective nodules. Proc. Natl. Acad. Sci. U.S.A. 82, 6231–6235.
Finan, TM, Kunkel, B, De Vos, GF, and Signer, ER (1986) Second symbiotic megaplasmid in Rhizobium meliloti carrying exopolysaccharide and thiamin synthesis genes. J. Bacteriol. 167, 66–72.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1987 Martinus Nijhoff Publishers, Dordrecht
About this chapter
Cite this chapter
Leigh, J.A., Reed, J., Walker, G.C. (1987). Symbiotic Mutants of Rhizobium Meliloti Which Produce Non-Succinylated Exopolysaccharide. In: Verma, D.P.S., Brisson, N. (eds) Molecular genetics of plant-microbe interactions. Current Plant Science and Biotechnology in Agriculture, vol 3. Springer, Dordrecht. https://doi.org/10.1007/978-94-009-4482-4_39
Download citation
DOI: https://doi.org/10.1007/978-94-009-4482-4_39
Publisher Name: Springer, Dordrecht
Print ISBN: 978-94-010-8496-3
Online ISBN: 978-94-009-4482-4
eBook Packages: Springer Book Archive