Abstract
Tumor formation by Agrobacterium tumefaciens involves the transfer of plasmid DNA from the bacteria to the plant host cell. One of the earliest steps in tumor formation appears to be the attachment of the bacteria to the surface of the plant cell. In general bacterial attachment does not appear to require the active participation of the plant host cell. Bacteria attach with only slightly altered kinetics to plant cells which have been killed by treatment with heat or glutaraldehyde(6). In contrast the bacteria appear to play an active role in the attachment process; bacteria which have been killed with heat or glutaraldehyde do not attach to plant cells(4). Bacterial attachment is required for virulence; bacterial mutants which fail to attach to plant cells are avirulent(2,4).
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References
Chernin LS, Lobanok EV, Fomicheva VV, Kartel NA: Crown gall-supressive IncW R plasmids cause a decrease in auxin production by Agrobacterium tumefaciens. Mol Gen Genet 195:195–199, 1984.
Douglas CJ, Halperin W, Nester EW: Agrobacterium tumefaciens mutants affected in attachment to plant cells. J Bacteriol 152:1265–1271, 1982.
Farrand SK, Kado CI, Ireland CR: Supression of tumorigenicity by the IncW R plasmid pSA. Mol Gen Genet 181:44–51, 1981.
Matthysse AG: Mutants of Agrobacterium tumefaciens which fail to bind to plant host cells. Submitted for publication.
Matthysse AG, Holmes KV, Gurlitz RHG: Elaboration of cellulose fibrils by Agrobacterium tumefaciens during attachment to carrot cells. J Bacteriol 145:583–595, 1981.
Matthysse AG, Holmes KV, Gurlitz RHG: Binding of Agrobacterium tumefaciens to carrot protoplasts. Physiol Plant Pathol 20:27–33, 1982.
New PB, Scott JJ, Ireland CR, Farrand SK, Lippincott BB, Lippincott JA: Plasmid pSA causes loss of LPS-mediated adherance in Agrobacterium. J Gen Microbiol 129:3657–3660, 1983.
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© 1987 Martinus Nijhoff Publishers, Dordrecht
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Matthysse, A.G. (1987). Effect of the Presence of the Plasmid pSA and of Auxin on the Attachment of Agrobacterium Tumefaciens to Plant Host Cells. In: Verma, D.P.S., Brisson, N. (eds) Molecular genetics of plant-microbe interactions. Current Plant Science and Biotechnology in Agriculture, vol 3. Springer, Dordrecht. https://doi.org/10.1007/978-94-009-4482-4_3
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DOI: https://doi.org/10.1007/978-94-009-4482-4_3
Publisher Name: Springer, Dordrecht
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