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Direct Analysis of the Invasiveness of Xanthomonas Campestris Mutants Generated by Tn4431, A Transposon Containing A Promoterless Luciferase Cassette for Monitoring Gene Expression

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Molecular genetics of plant-microbe interactions

Part of the book series: Current Plant Science and Biotechnology in Agriculture ((PSBA,volume 3))

Abstract

Difficulties in the detection and quantification of small numbers of bacteria have long been apparent. In most cases, detection of invading bacteria is not possible prior to the onset of visible symptoms. Even in the presence of symptoms, sampling is done by extracting bacteria from the invaded tissues. The procedure is always disruptive of the ongoing disease process and the information obtained is dated as it often depends on the formation of colonies. The same difficulties apply in studying bacterial genetics in planta.

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References

  1. Hastings, J. W. (1977) Bacterial Bioluminescence. Ann Rev. Microbiol. 31:549–595.

    Article  CAS  Google Scholar 

  2. Engebrecht, J., Nealson, K. and Silverman, M. (1983) Bacterial bioluminescence: isolation and genetic analysis of functions from Vibrio fischeri, Cell, 32:773–781.

    Article  PubMed  CAS  Google Scholar 

  3. Engebrecht, J., Simon, M. and Silverman, M. (1985) Measuring gene expression with light, Science, 227:1345–1347.

    Article  PubMed  CAS  Google Scholar 

  4. Wall, L., Byers, D. and Meighen, E. (1984) In vivo and in vitro acylation of polypeptides in Vibrio harveyi: identification of proteins involved in aldehyde production for bioluminescence, J. Bacteriol., 159:720–724.

    PubMed  CAS  Google Scholar 

  5. Shaw, J. and Kado, C. (1986) Development of a Vibrio Bioluminescence gene-set to monitor phytopathogenic bacteria during the ongoing disease process in a non-disruptive manner, Bio/technology, 4:560–564.

    Article  CAS  Google Scholar 

  6. Maniatis, T., Fritsch, E. and Sambrook, J. (1982) Molecular Cloning: A Laboratory Manual, Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.

    Google Scholar 

  7. Figurski, D., and Helinski, D. (1979) Replication of an origin containing derivative of plasmid RK2 dependent on a plasmid function provided in trans. Proc. Natl. Acad. Sci., USA, 76:1648–1652.

    Article  PubMed  CAS  Google Scholar 

  8. Schmitt, R., Bernhard, E. and Mattes, R. (1979) Characterization of Tn1721, a new transposon containing tetracycline resistance genes capable of amplification, Mol. Gen. Genet. 172:53–65.

    Article  PubMed  CAS  Google Scholar 

  9. Zaitlin, D. (1984) Ph. D. dissertation, Department of Biochemistry and Biophysics, University of California at Davis, Davis, CA, USA.

    Google Scholar 

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© 1987 Martinus Nijhoff Publishers, Dordrecht

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Shaw, J.J., Kado, C.I. (1987). Direct Analysis of the Invasiveness of Xanthomonas Campestris Mutants Generated by Tn4431, A Transposon Containing A Promoterless Luciferase Cassette for Monitoring Gene Expression. In: Verma, D.P.S., Brisson, N. (eds) Molecular genetics of plant-microbe interactions. Current Plant Science and Biotechnology in Agriculture, vol 3. Springer, Dordrecht. https://doi.org/10.1007/978-94-009-4482-4_12

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  • DOI: https://doi.org/10.1007/978-94-009-4482-4_12

  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-94-010-8496-3

  • Online ISBN: 978-94-009-4482-4

  • eBook Packages: Springer Book Archive

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