Abstract
We have used P element-mediated transformation of Drosophila embryos as an assay system to determine the role of different DNA sequences located in the 5′ region of the Drosophila hsp28 gene on its ability to be induced by heat shock and by the steroid hormone ecdysone. Flies that contain the transformed gene with 1.1 kb of 5′ information express normal levels of RNA after developmental induction by ecdysone but they accumulate lower than wildtype amounts of RNA after heat shock. Transformants which contain 227 by of 5′ information show ecdysone and temperature-induced expression of the foreign gene, but the RNA accumulates at lower than wildtype levels. Finally, deletion of the sequences located between −227 and −124 results in abolishment of transcription induction by heat shock but not by ecdysone. These results suggest that the sequences necessary for heat shock and hormone regulation of transcription are located in different regions of the hsp28 gene.
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© 1986 Martinus Nijhoff Publishers, Dordrecht
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Hoffman, E., Corces, V. (1986). Identification of cis-acting regulatory sequences by P element-mediated germline transformation of Drosophila embryos. In: Augustine, P.C., Danforth, H.D., Bakst, M.R. (eds) Biotechnology for Solving Agricultural Problems. Beltsville Symposia in Agricultural Research, vol 10. Springer, Dordrecht. https://doi.org/10.1007/978-94-009-4396-4_12
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DOI: https://doi.org/10.1007/978-94-009-4396-4_12
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