Abstract
A closed system consisting of an 40 cm3 assimilation chamber furnished with a membrane inlet from the liquid phase connected to a mass spectrometer and an oxygen Clark type electrode were used to measure 16O2 evolution, 18O2 uptake and net oxygen evolution by air grown Chlamydomonas reinhardtii suspensions in the light (600 µmol photons/m2/s). At low DIC the algal suspension was exposed to 18O2 and it was found that the rate of O2 uptake (U0) equalled the rate of O2 production (E0) at DIC compensation. Upon injection of dissolved inorganic carbon (DIC) saturating for photosynthesis (150 mmol/m3), E0 was increased fivefold over the rate measured at DIC compensation. At the same time U0 was 32–40% lower than U0 during DIC compensation (55–90µmol/mg Chl/h). On the contrary, stepwise addition of DIC over a period of 20 min led to an increase in both E0 and U0. The validity of the mass spectrometric measurements and possible explanations for these conflicting results are discussed.
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Abbreviations
- Chl:
-
chlorophyll
- DIC:
-
dissolved inorganic carbon
- E0 :
-
rate of oxygen evolution in the light
- MS:
-
mass spectrometer
- NET:
-
rate of net oxygen evolution in the light
- PCO:
-
photosynthetic carbon oxidation
- PFR:
-
photon fluence rate
- Rubisco:
-
ribulose-1,5-bisphosphate carboxylase/oxygenase
- PubP:
-
ribulose-1,5-bisphosphate
- U0 :
-
rate of oxygen uptake in the light
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© 1986 Martinus Nijhoff Publishers, Dordrecht
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Sültemeyer, D.F., Fock, H.P. (1986). Mass Spectrometric Analysis of Photosynthetic Oxygen Evolution and Uptake by Chlamydomonas Reinhardtii. In: Marcelle, R., Clijsters, H., van Poucke, M. (eds) Biological Control of Photosynthesis. Advances in Agricultural Biotechnology, vol 19. Springer, Dordrecht. https://doi.org/10.1007/978-94-009-4384-1_12
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DOI: https://doi.org/10.1007/978-94-009-4384-1_12
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