Summary
The technique of 15N isotope dilution was used to verify that nitrogen was fixed and transferred to the plant by Klebsiella pneumoniae strain Pp in association with Poa pratensis or Triticum aestivum. Surface sterilized, sprouting seeds were inoculated with K. pneumoniae and grown in sand in modified Leonard jars. Potassium nitrate enriched with 15N was used to provide N concentrations ranging from 10–40 mg N l-1 nutrient solution. After 10–18 weeks the shoots and roots were analyzed separately for dry matter, N content, total N, and atom % 15N excess. The acetylene reduction technique was used to test for the presence of N2-fixing organisms on the roots. The data from 15N isotope dilution demonstrated that up to 33.8% of N in the shoots of P. pratensis and 15.9% in those of T. aestivum were derived from associative N2 fixation by K. pneumoniae. In most experiments the dry matter yield, N content, and total N yield of the shoots of P. pratensis were increased by K. pneumoniae inoculation, whereas inoculation had no significant effect on the dry matter yield, N content or total N of the shoots of T. aestivum.
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© 1986 Martinus Nijhoff Publishers, Dordrecht
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Haahtela, K., Kari, K. (1986). The role of root-associated Klebsiella pneumoniae in the nitrogen nutrition of Poa pratensis and Triticum aestivum as estimated by the method of 15N isotope dilution. In: Skinner, F.A., Uomala, P. (eds) Nitrogen Fixation with Non-Legumes. Developments in Plant and Soil Sciences, vol 21. Springer, Dordrecht. https://doi.org/10.1007/978-94-009-4378-0_19
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DOI: https://doi.org/10.1007/978-94-009-4378-0_19
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