Summary
A clone bank of an indigenous plasmid of Enterobacter agglomerans containing structural nitrogen-fixation (nif) genes was established in a non-mobilisable, multicopy derivative of the cosmid vector pHC79 The restriction enzyme Bam HI was used to establish the clone bank and it was found that 96% of the clones contained inserts. The clones containing nif-genes were identified by Southern hybridisation using Klebsiella pneumoniae nif DNA (Kp nifHDKY) as the radioactive probe. The nif-genes of E. agglomerans showed extensive homology to those of K. pneumoniae but the restriction enzyme fragment patterns of the nif-genes of E. agglomerans were different. The plasmid borne nif-genes of E. agglomerans are clustered as in K. pneumoniae.
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© 1986 Martinus Nijhoff Publishers, Dordrecht
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Singh, M., Klingmüller, W. (1986). Cloning of pEA3, a large plasmid of Enterobacter agglomerans containing nitrogenase structural genes. In: Skinner, F.A., Uomala, P. (eds) Nitrogen Fixation with Non-Legumes. Developments in Plant and Soil Sciences, vol 21. Springer, Dordrecht. https://doi.org/10.1007/978-94-009-4378-0_18
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DOI: https://doi.org/10.1007/978-94-009-4378-0_18
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