Summary
Reported non-stoichiometry in the reduction of nitrate to nitrite was investigated in leaves of Zea mays L.. The nitrogen balance sheet for in vitro nitrate assimilation was influenced by enzyme protectants in the extraction media and by the method employed to terminate the reaction. A number of limitations were found in the generally accepted in vitro nitrate reductase assay, in particular the presence of endogenous components which interfered with the assay of nitrite were considered. A stoichiometric balance for the in vitro reduction of nitrate to nitrite was obtained when interfering factors were minimized. The absence of back reactions from ammonia in the assay was confirmed.
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Abbreviations
- BSA:
-
Bovine serum albumin
- DTT:
-
DL-dithiothreitol
- EDTA:
-
Ethylenediamine tetra-acetic acid disodium salt
- FAD:
-
Flavin adenine dinucleotide
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© 1986 Martinus Nijhoff Publishers, Dordrecht/Boston/Lancaster
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Brunswick, P., Cresswell, C.F. (1986). An evaluation of the stoichiometry of in vitro nitrate assimilation in Zea mays . In: Lambers, H., Neeteson, J.J., Stulen, I. (eds) Fundamental, Ecological and Agricultural Aspects of Nitrogen Metabolism in Higher Plants. Developments in Plant and Soil Sciences, vol 19. Springer, Dordrecht. https://doi.org/10.1007/978-94-009-4356-8_20
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DOI: https://doi.org/10.1007/978-94-009-4356-8_20
Publisher Name: Springer, Dordrecht
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