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TLC and HPLC methods for analysis of trichothecenes in commodities

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Part of the book series: Current Topics in Veterinary Medicine and Animal Science ((CTVM,volume 33))

Abstract

The problems presented to the analytical chemist in the analysis of foods and feeds for trichothecenes are unique because the chemical structures and therefore the chemical and physical properties vary widely among the individual members of this large group of natural products. Ueno [29] has grouped the trichothecenes in four general categories (A, B, C, D) based on chemical structure (see Fig. 1).

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References

  1. Bata, A., Vanyi, A., Laszity, R. & Galacz, J., 1984. Determination of trichothecene toxins in foods and feeds. J. Chromatogr. 286:357–363.

    Article  CAS  Google Scholar 

  2. Baxter, J., Terhune, S. & Qureshi, S., 1984. Use of chromotropic acid for improved thin layer chromatographic visualization of trichothecene mycotoxins. J. Chromatogr. 261:130–133.

    Article  Google Scholar 

  3. Bennett, G., Peterson, R., Plattner, R. & Shotwell, O., 1981. Isolation and purification of deoxynivalenol and a new trichothecene by high pressure liquid chromatography. J. Am. Oil Chem. Soc. XX:1002A-1005A.

    Article  Google Scholar 

  4. Chang, H., Dries, J., Larson, P. & Patel, H., 1984. Rapid determination of deoxynivalenol (vomitoxin) by liquid chromatography using modified Romer column cleanup. J. Assoc. Off. Anal. Chem. 67:52–54.

    PubMed  CAS  Google Scholar 

  5. Cole, R. & Cox, J., 1981. Handbook of Toxic Fungal Metabolites. Academic Press, New York.

    Google Scholar 

  6. Ehrlich, K., Lee, L. & Ciegler, A., 1983. A simple, sensitive method for detection of vomitoxin (deoxynivalenol) using reversed phase, high performance liquid chromatography. J. Liq. Chromatogr. 6:833–843.

    Article  CAS  Google Scholar 

  7. Eppley, R., 1979. Trichothecenes and their analysis. J. Am. Oil Chem. Soc. 56:824–829.

    Article  CAS  Google Scholar 

  8. Eppley, R., 1984. Associate Referee Report. 98th Annual Meeting of the AOAC, Oct. 29-Nov. 2, Washington, D.C.

    Google Scholar 

  9. Gimeno, A., 1979. Thin layer chromatographic determination of aflatoxins, ochratoxins, sterigmatocystin, zearalenone, citrinin, T-2 toxin, diacetoxyscirpenol, penicillic acid, patulin and penitrem A. J. Assoc. Off. Anal. Chem. 62:579–585.

    PubMed  CAS  Google Scholar 

  10. Ichinoe, M., Kurata, H., Suguira, Y. & Ueno, Y., 1983. Chemotaxonomy of Gibberella zeae with special reference to production of trichothecenes and zearalenone. Appl. Environ. Microbiol. 46:1364–1369.

    PubMed  CAS  Google Scholar 

  11. Katsonis, T. & Ellison, R., 1975. Assay and relationship of HT-2 toxin and T-2 toxin formation in liquid culture. Appl. Environ. Microbiol. 30:33–37.

    Google Scholar 

  12. Miller, J., Young, J. & Trenholm, H., 1983. Fusarium toxins in field corn. I. Time course of fungal growth and production of deoxynivalenol and other mycotoxins. Can. J. Bot. 61:3080–3087.

    CAS  Google Scholar 

  13. Mirocha, C., 1979. Trichothecene toxins produced by Fusarium. In: Conference on Mycotoxins in Animal Feeds and Grains Related to Animal Health, pp 289–373. National Technical Information Service, PB-300, Washington, DC.

    Google Scholar 

  14. Pathre, S. Mirocha, C., 1977. Assay methods for trichothecenes and review of their natural occurrence. In: Mycotoxins in Human and Animal Health (Rodricks, J., Hesseltine, C., & Mehlman, M., eds.), pp. 229–253. Pathotox, Park Forest South, IL.

    Google Scholar 

  15. Patterson, D. & Roberts, B., 1979. Mycotoxins in animal feedstuffs: Sensitive thin layer chromatographic detection of aflatoxin, ochratoxin A, sterigmatocystin, zearalenone, and T-2 toxin. J. Assoc. Off. Anal. Chem. 62:1265–1267.

    PubMed  CAS  Google Scholar 

  16. Pohland, A., Thorpe, C. & Sphon, J., 1984. The analytical chemistry of deoxynivalenol. In: Toxigenic Fungi — Their Toxins and Health Hazards (Kurata, H., & Ueno, Y., eds.), pp. 217–230. Elsevier, New York.

    Google Scholar 

  17. Romer, T., Greaves, D. & Gibson, G., 1981. Vomitoxin analysis. 6th Spring Workshop of the AOAC, May 12–14, Ottawa, Ontario, Canada.

    Google Scholar 

  18. Sano, A., Asabe, Y., Takitani, S. & Ueno, Y., 1982. Fluorodensitometric determination of trichothecene mycotoxins with nicotinamide and 2-acetylpyridine on a silica gel layer. J. Chromatogr. 235:257–265.

    Article  CAS  Google Scholar 

  19. Schmidt, R., Bieger, A., Zeigenhagen, E.E., & Dose, K., 1981. Determination of T-2 toxin in vegetable foodstuffs. Z. Anal. Chem. 308:133–136.

    Article  CAS  Google Scholar 

  20. Schmidt, R., Zeigenhagen, E. & Dose, K., 1982. Quantitative determination of trichothecenes: HT-2 toxin in moldy cereal grains and vegetable foods. Lebens. Untersuch. Forsch. 175:169–171.

    Article  CAS  Google Scholar 

  21. Schweigehardt, H., Bohm, J., Abdelhamid, A. & Leibetseder, J., 1980. Analysis of fusariotoxins zearalenone and vomitoxin (deoxynivalenol) in human foods and animal feeds by high performance liquid chromatography (HPLC). Chromatographia 13:447–450.

    Article  Google Scholar 

  22. Stack, M. & Eppley, R., 1982. Analysis of trichothecenes by TLC. In: Advances in Thin Layer Chromatography (Touchstone, J., ed.), pp. 495–502. John Wiley and Sons, New York.

    Google Scholar 

  23. Takeda, Y., Isohata, E., Amano, R. & Uchiyama, M., 1979. Simultaneous extraction and fractionation and thin layer chromatographic determination of 14 mycotoxins in grains. J. Assoc. Off. Anal. Chem. 62:573–578.

    PubMed  CAS  Google Scholar 

  24. Takitani, S., 1979. Spectrodensitometric determination of trichothecene mycotoxins with 4-(P-nitrobenzyl) pyridine on silica gel thin layer chromatograms. J. Chromatogr. 172:335–342.

    Article  PubMed  CAS  Google Scholar 

  25. Takitani, S. & Asabe, Y., 1983. Thin layer chromatographic analysis of trichothecene mycotoxins. In: Trichothecenes: Chemical, Biological and Toxicological Aspects (Ueno, Y., ed.), pp 113–124. Elsevier, New York.

    Google Scholar 

  26. Thorpe, C., Thomas, F., Salinger, D. & Pohland, A., 1984. Analysis for trichothecenes by liquid chromatography with UV and electrochemical detectors. Pittsburgh Conference on Analytical Chemistry and Applied Spectroscopy, March 3–5, Atlantic City, NJ, Abstract No. 635.

    Google Scholar 

  27. Trucksess, M., Nesheim, S. & Eppley, R., 1984. Thin layer chromatographic determination of deoxynivalenol in wheat and corn. J. Assoc. Off. Anal. Chem. 67:40–43.

    PubMed  CAS  Google Scholar 

  28. Trucksess, M. Wood, G.E., Eppley, R.M., Young, K., Cohen, C.K. & Page S.W., 1984. Deoxynivalenol in grains and grain products. Annual Meeting, Biodeterioration Society, August 5–10, George Washington University, Washington, DC.

    Google Scholar 

  29. Ueno, Y., 1979. Trichothecenes: Overview address. In: Mycotoxins in Human and Animal Health (Rodricks, J., Hesseltine, C. & Mehlman, M., eds.), pp 189–207. Pathotox, Park Forest South, IL.

    Google Scholar 

  30. Visconti, A. & Bottalico, A., 1983. Detection of Fusarium trichothecenes (nivalenol, deoxynivalenol, fusarenone and 3-acetyldeoxynivalenol) by high-performance liquid chromatography. Chromatographia 17:97–100.

    Article  CAS  Google Scholar 

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© 1986 Martinus Nijhoff Publishers, Dordrecht

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Pohland, A.E., Thorpe, C.W., Trucksess, M.W., Eppley, R.M. (1986). TLC and HPLC methods for analysis of trichothecenes in commodities. In: Richard, J.L., Thurston, J.R. (eds) Diagnosis of Mycotoxicoses. Current Topics in Veterinary Medicine and Animal Science, vol 33. Springer, Dordrecht. https://doi.org/10.1007/978-94-009-4235-6_23

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  • DOI: https://doi.org/10.1007/978-94-009-4235-6_23

  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-94-010-8380-5

  • Online ISBN: 978-94-009-4235-6

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