Abstract
It was the development of enzymic methods of protoplast isolation in the 1960s that allowed the preparation of large numbers of isolated protoplasts. An isolated protoplast may be defined as a plant cell that has had its outer wall removed; therefore the only boundary between the cell contents and the external environment is the plasma membrane. The removal of the rigid cell wall causes the protoplast to take on a spherical conformation in liquid culture (conformation of minimum energy) and also opens the way to technologies that cannot be applied to other plant cells.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Dodds, J. H. and Bengochea, T. (1983) Outlook on Agric., 12, 16–21.
Bucke, C., Walker, D. A. and Baldry, C. W. (1966) Biochem. J., 101, 636–41.
Carlson, P. S. (1973) Proc. Natl. Acad. Sei. (USA), 70, 598–602.
Potrykus, I. (1973) Z. Pflanzenphysiol., 70, 364–66.
Bonnett, H. T. and Eriksson, T. (1974) Planta, 120, 71–79.
Davey, M. R. and Short, K. C. (1972) Protoplasma, 75, 199–203.
Davey, M. R. and Cocking, E. C. (1972) Nature (Lond.), 239, 455–56.
Burgoon, A. C. and Bottino, P. J. (1976) J. Hered., 67, 223–26.
Cocking, E. C. (1966) Planta, 68, 206–14.
Coutts,R. H. A. (1973) in Protoplasts at fusion de cellules nomatiques vegetales. Colloq. Intern. Centre Natl. Rech. Sci. Paris,pp.353–65.
Otsuki, Y. and Takebe, I. (1973) Virology, 52, 433–38.
Holl, F. B., Gamborg, O. L., Ohyama, K. and Pelcher, L. (1974) in Tissue Culture and Plant Science (ed. H. E. Street), Academic Press, London, pp. 301–27.
Doy, C. H. Gresshoff, P. M. and Rulfe, B. G. (1973) Proc. Natl. Acad. Sei. (USA), 70, 723–26.
Johnson, C. B., Grierson, D. and Smith, H. (1973) Nature (New Biol), 244, 105–106.
Author information
Authors and Affiliations
Rights and permissions
Copyright information
© 1986 Teresa Bengochea and John H. Dodds
About this chapter
Cite this chapter
Bengochea, T., Dodds, J.H. (1986). Uptake of foreign materials. In: Plant Protoplasts. Springer, Dordrecht. https://doi.org/10.1007/978-94-009-4095-6_6
Download citation
DOI: https://doi.org/10.1007/978-94-009-4095-6_6
Publisher Name: Springer, Dordrecht
Print ISBN: 978-94-010-8317-1
Online ISBN: 978-94-009-4095-6
eBook Packages: Springer Book Archive