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Solubilization and purification of membrane proteins

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Abstract

Recently, there has been an enormous increase in the number of studies on the solubilization and purification of membrane proteins. One of the reasons for this phenomenon is the increase in researchers’ interest in problems related to biological membranes such as energy transduction, cell-to-cell recognition and secretion. Another reason is the very rapid development of techniques including new materials and apparatus, for purifying proteins. Availability of these ‘secret weapons’ from commercial sources may also have encouraged researchers to attempt purification of new membrane proteins. So at the present, purification of membrane proteins is not much more difficult than purification of soluble proteins.

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References

General references

  • Biomembranes (since 1971), Plenum Press, New York.

    Google Scholar 

  • Current Topics in Bioenergetics (since 1966), Academic Press, New York.

    Google Scholar 

  • Current Topics in Membranes and Transport (since 1970), Academic Press, New York.

    Google Scholar 

  • The Enzymes of Biological Membranes (1976), (ed A. Martonosi), Plenum Press, New York.

    Google Scholar 

  • Methods in Membrane Biology (since 1974), Plenum Press, New York.

    Google Scholar 

  • Methods in Enzymology (since 1955), (Vols. 10,23, 31, 32, 52–56, 81, 88,96, and 97), Academic Press, New York.

    Google Scholar 

  • Nelson, D. R. and Robinson, N. C. (1983) Methods Enzymol., 97, 571–618.

    Article  Google Scholar 

Articles cited in the text

  • Anderson, W. M. and Fisher, R. R. (1978) Arch. Biochem. Biophys., 187, 180–90.

    Article  Google Scholar 

  • Ashani, Y. and Catravas, G. N. (1980) Anal. Biochem., 109, 55–62.

    Article  Google Scholar 

  • Azari, M. R. and Wiseman, A. (1982) Anal. Biochem., 122, 129–38.

    Article  Google Scholar 

  • Azzi, A., Brodbeck, U. and Zahler, P. (eds) (1981) Membrane Proteins. A Laboratory Manual, Springer-Verlag, Berlin.

    Google Scholar 

  • Bill, K., Broger, C. and Azzi, A. (1982) Biochim. Biophys. Acta, 679, 28–34.

    Article  Google Scholar 

  • Böhme, H., Brütsch, S., Weithmann, G. and Böger, P. (1980) Biochim. Biophys. Acta, 590, 248–60.

    Article  Google Scholar 

  • Brudig, G. W., Worland, S. T. and Sauer, K. (1983) Proc. Natl. Acad. Sci. USA, 80, 683–6.

    Article  Google Scholar 

  • Capony, J.-P., Rinaldi, M. L., Guilleux, F. and Demaille, J. G. (1983) Biochim. Biophys. Acta, 728, 83–91.

    Article  Google Scholar 

  • Carson, S. D. and Königsberg, W. H. (1981) Anal. Biochem., 116, 398–401.

    Article  Google Scholar 

  • Célis, H. (1980) Biochem. Biophys. Res. Commun., 92, 26–31.

    Article  Google Scholar 

  • Chang, H. W. and Bock, E. (1980) Anal. Biochem., 104, 112–17.

    Article  Google Scholar 

  • Cheetham, P. S. J. (1979) Anal. Biochem., 92, 26–31.

    Article  Google Scholar 

  • Cottingham, I. R. and Ragan, C. I. (1980) Biochem. J., 192, 9–18.

    Google Scholar 

  • Cresswell, P. (1979) J. Biol. Chem., 254, 414–19.

    Google Scholar 

  • Davies, J. T. (1961) in Interfacial Phenomena, Academic Press, New York.

    Google Scholar 

  • Davis, K. A. and Hatefi, Y. (1971) Biochemistry, 10, 2509–16.

    Article  Google Scholar 

  • De Vrij, W., Azzi, A. and Konigs, W. N. (1983) Eur. J. Biochem., 131, 97–103.

    Article  Google Scholar 

  • Dey, A. C., Rahal, S., Rimsey, R. L. and Senciall, I. R. (1981) Anal. Biochem., 110, 373–9.

    Article  Google Scholar 

  • Fujita-Yamaguchi, Y., Choi, S., Sakamoto, Y. and Itakura, K. (1983) J. Biol. Chem., 258, 5045–9.

    Google Scholar 

  • Gennis, R. B., Casey, R. P., Azzi, A. and Ludwig, B. (1982) Eur. J. Biochem., 125, 189–95.

    Article  Google Scholar 

  • Gotoh, O., Tagashira, Y., Iizuka, T. and Fujii-Kuriyama, Y. (1983) J. Biochem. (Tokyo), 93, 807–17.

    Google Scholar 

  • Guengerich, F. P. and Martin, M. V. (1980) Arch. Biochem. Biophys., 205, 365–79.

    Article  Google Scholar 

  • Gurtabay, I. G., Azagra, E., Gutiérrez-Arranz, A., Milicua J. C. G. and Goñi, F. M. (1979) Biochem. Soc. Trans., 7, 150–2.

    Google Scholar 

  • Hamaguchi, K. and Geiduscheck, E. P. (1962) J. Amer. Chem. Soc., 84, 1329.

    Article  Google Scholar 

  • Hanatani, M., Nishifuji, K., Futai, M. and Tsuchiya, T. (1983) Seikagaku, 55, 651 (in Japanese).

    Google Scholar 

  • Hanstein, W. G., Davis, K. A. and Hatefi, Y. (1971) Arch. Biochem. Biophys. 147, 534–44.

    Article  Google Scholar 

  • Helenius, A. and Simons, K. (1975) Biochim. Biophys. Acta, 415, 29–79.

    Google Scholar 

  • Henderson, P. J. F., Kagawa, Y. and Hirata, H. (1983) Biochim. Biophys. Acta, 732, 204–9.

    Article  Google Scholar 

  • Hildreth, J. E. K. (1982) Biochem. J., 207, 363–6.

    Google Scholar 

  • Hirata, H. (1979) Methods Enzymol, 56, 430–5.

    Article  Google Scholar 

  • Hirata, H., Sone, N., Yoshida, M. and Kagawa, Y. (1977) J. Supramol. Struct., 6, 77–84.

    Article  Google Scholar 

  • Hirata, H., Kambe, T. and Kagawa, Y. (1984) J. Biol. Chem., 259 (17) 10653–6.

    Google Scholar 

  • Hjeimeland, L. M., Nebert, D. W. and Chrambach, A. (1979) Anal. Biochem., 95, 201–8.

    Article  Google Scholar 

  • Holloway, P. W. (1973) Anal. Biochem., 53, 304–8.

    Article  Google Scholar 

  • Huang, K.-S., Bayley, H., Liao, M. J., London, E. and Khorana, H. G. (1981) J. Biol. Chem., 256, 3802–9.

    Google Scholar 

  • Ikai, A. (1980) J. Biochem. (Tokyo), 88, 1349–57.

    Google Scholar 

  • Imai, Y. and Sato, R. (1974) J. Biochem. (Tokyo), 75, 689–98.

    Google Scholar 

  • Jørgensen, P. L. (1974) Meths Enzymol, 32, 277–90.

    Article  Google Scholar 

  • Jørgensen, P. L. (1982) Biochim. Biophys. Acta, 694, 27–68.

    Google Scholar 

  • Kaback, H. R. (1983) J. Membr. Biol, 76, 95–112.

    Article  Google Scholar 

  • Kagawa, Y. (1972) Biochim. Biophys. Acta, 265, 297–338.

    Google Scholar 

  • Kagawa, Y., Hirata, H., Ohta, S., Ishizuka, M. and Karube, Y. (1986) Char Ion Transport through Membranes (eds K. Yagi and B. Pullman) Academic Press, New York, in press.

    Google Scholar 

  • Kagawa, Y. and Racker, E. (1971) J. Biol Chem., 246, 5477–87.

    Google Scholar 

  • Kanazawa, H., Miki, T., Tamura, F., Yura, T. and Futai, M. (1979) Proc. Natl Acad. Sci. USA, 76, 1126–30.

    Article  Google Scholar 

  • Koepsell, H., Menuhr, H., Ducis, I. and Wissmiiller, T. F. (1983) J. Biol Chem., 258, 1888–94.

    Google Scholar 

  • Koval, S. F. and Murray, K. G. E. (1983) Can. J. Microbiol, 29, 146–50.

    Article  Google Scholar 

  • Lichtenberg, D., Robson, R. J. and Dennis, E. A. (1983) Biochim. Biophys. Acta, 737, 285–304.

    Google Scholar 

  • Loizaga, B., Gurtubay, I. G., Macarulla, J. M., Goni, F. M. and Gómez, J. C. (1979) Biochem. Soc. Trans., 7, 148–50.

    Google Scholar 

  • MacPherson, A. J. S., Jones-Mortimer, M. C. and Henderson, P. J. F. (1981) Biochem. J., 196, 269–83.

    Google Scholar 

  • MacPherson, A. J. S., Jones-Mortimer, M. C., Home, P. and Henderson, P. J. F. (1983) J. Biol Chem., 258, 4390–6.

    Google Scholar 

  • Navarette, R. and Serrano, R. (1983) Biochim. Biophys. Acta, 728, 403–8.

    Article  Google Scholar 

  • Newby, A. C., Chrambach, A. and Bailyes, E. M. (1982) Tech. Life Sci. Sect. Biochem., B409, 22 pp.

    Google Scholar 

  • Newman, M. J., Foster, D. L., Wilson, T. H. and Kaback, H. R. (1981) J. Biol Chem., 256, 11804–8.

    Google Scholar 

  • O’Farrell, P. Z., Goodman, H. M. and O’Farrell, P. H. (1977) Cell, 12, 1133–42.

    Article  Google Scholar 

  • Omura, T. and Takesue, S. (1970a) J. Biochem. (Tokyo), 67, 249–57.

    Google Scholar 

  • Omura, T. and Takesue, S. (1970b) J. Biochem. (Tokyo), 67, 267–76.

    Google Scholar 

  • Oppenheimer, C. L. and Czech, M. P. (1983) J. Biol Chem., 258, 8539–42.

    Google Scholar 

  • Ozawa, T., Suzuki, H. and Tanaka, M. (1980) Proc. Natl Acad. Sci. USA, 77, 928–30.

    Article  Google Scholar 

  • Ozawa, T., Tanaka, M. and Wakabayashi, T. (1982) Proc. Natl. Acad. Sci. USA, 79, 7175–9.

    Article  Google Scholar 

  • Peterson, G. L. and Hokin, L. E. (1980) Biochem. J., 192, 107–18.

    Google Scholar 

  • Poole, R. K. (1983) Biochim. Biophys. Acta, 726, 205–43.

    Google Scholar 

  • Racker, E. (1983) Fed. Proc., 42, 2899–2909.

    Google Scholar 

  • Racker, E., Violand, B., O’Neal, S., Alfonzo, M. and Telford, J. (1979) Arch. Biochem. Biophys., 198, 470–7.

    Google Scholar 

  • Rivas, E., Pasdeloup, N. and le Maire, M. (1982) Anal. Biochem., 123, 194–200.

    Article  Google Scholar 

  • Rivnay, B. and Metzger, H. (1982) J. Biol. Chem., 257, 12800–8.

    Google Scholar 

  • Sawyer, W. H. and Puckridge, J. (1973) J. Biol. Chem., 248, 8429–33.

    Google Scholar 

  • Scheule, R. K. and Gaffney, B. J. (1981) Anal. Biochem., 117, 61–6.

    Article  Google Scholar 

  • Schneider, E. and Altendorf, K. (1982) Eur. J. Biochem., 126, 149–53.

    Article  Google Scholar 

  • Serrano, A. and Rivas, J. (1982) Anal. Biochem., 126, 109–15.

    Article  Google Scholar 

  • Shinoda, K., Nakagawa, T., Tamamushi, B. and Isemura, T. (1963) Colloidal Surfactants, Academic Press, New York.

    Google Scholar 

  • Sigel, E., Stephenson, F. A., Mamalaki, C. and Barnard, E. A. (1983) J. Biol. Chem., 258, 6965–71.

    Google Scholar 

  • Sone, N., Kagawa, Y. and Orii, Y. (1983) J. Biochem. (Tokyo), 93, 1329–36.

    Google Scholar 

  • Sone, N., Ohyama, T. and Kagawa, Y. (1979) FEBS Lett., 106, 39–42.

    Article  Google Scholar 

  • Sone, N. and Yanagita, Y. (1982) Biochim. Biophys. Acta, 682, 216–26.

    Article  Google Scholar 

  • Sone, N., Yoshida, M., Hirata, H. and Kagawa, Y. (1975) J. Biol. Chem., 250, 7917–23.

    Google Scholar 

  • Sone, N., Yoshida, M., Hirata, H. and Kagawa, Y. (1978) Proc. Natl. Acad. Sci. USA, 75, 4219–23.

    Article  Google Scholar 

  • Tanford, C. and Reynolds, J. A. (1976) Biochim. Biophys. Acta, 457, 133–70.

    Google Scholar 

  • Tracy, R. P. and Chan, S. H. P. (1979) Biochim. Biophys. Acta, 576, 109–17.

    Google Scholar 

  • T’sai, A.-L. and Palmer, G. (1982) Biochim. Biophys. Acta, 681, 484–95.

    Article  Google Scholar 

  • Tsuchida, S. and Sato, K. (1983) Biochim. Biophys. Acta, 756, 341–8.

    Google Scholar 

  • Tsuchiya, T., Misawa, A., Miyake, Y., Yamasaki, K. and Niiya, S. (1982) FEBS Lett., 142, 231–4.

    Article  Google Scholar 

  • Von Dippe, P. and Levy, D. (1983) J. Biol. Chem., 258, 8896–901.

    Google Scholar 

  • Watanabe, H., Kamita, Y., Nakamura, T. and Yamanaka, T. (1979) Biochim. Biophys. Acta, 547, 70–8.

    Google Scholar 

  • Weber, K. and Kuter, J. (1971) J. Biol. Chem., 246, 4504–9.

    Google Scholar 

  • Weiss, H. and Juchs, B. (1978) Eur. J. Biochem., 88, 17–28.

    Article  Google Scholar 

  • Weiss, H. and Kolb, H. J. (1979) Eur. J. Biochem., 99, 139–49.

    Article  Google Scholar 

  • West, S. B., Huang, M.-T., Miwa, G. T. and Lu, A. Y. H. (1979) Arch. Biochem. Biophys., 193, 42–50.

    Article  Google Scholar 

  • Womack, M. D., Kendall, D. A. and MacDonald, R. C. (1983) Biochim. Biophys. Acta, 733, 210–15.

    Article  Google Scholar 

  • Wyman, J. (1964) Adv. Protein Chem., 19, 224–86.

    Google Scholar 

  • Yamanaka, T., Kamita, Y. and Fukumori, Y. (1981) J. Biochem. (Tokyo), 89, 265–73.

    Google Scholar 

  • Yanagita, Y., Sone, N. and Kagawa, Y. (1983) Biochem. Biophys. Res. Commun., 113, 575–80.

    Article  Google Scholar 

  • Yoda, A. and Yoda, Y. (1981) Anal. Biochem., 110, 82–8.

    Article  Google Scholar 

  • Yoshida, M., Sone, N., Hirata, H. and Kagawa, Y. (1975) J. Biol. Chem., 250, 7910–16.

    Google Scholar 

  • Yoshida-Momoi, M. and Lennon, V. A. (1982) J. Biol. Chem., 257, 12757–64.

    Google Scholar 

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Yanagita, Y., Kagawa, Y. (1986). Solubilization and purification of membrane proteins. In: Ragan, C.I., Cherry, R.J. (eds) Techniques for the Analysis of Membrane Proteins. Springer, Dordrecht. https://doi.org/10.1007/978-94-009-4085-7_3

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