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Future Directions in Polymer Colloids pp 321–354Cite as

Biomedical Applications of Polymer Particles with Emphasis on Cell Separation

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Part of the book series: NATO ASI Series ((NSSE,volume 138))

Abstract

Applications of polymer particles in the biomedical field have been concentrated in the past primarily in the areas of blood flow determination and in vitro immunoassays. Microspheres have been employed for the determination of myocardial, renal, cerebral and other blood flow and perfusion rates (Phibbs, et al, 1967; Hollenberg, 1975; Hoffbrand and Forsyth, 1969). Polymer particles, and latexes in particular have been extensively used in immunoassays, starting in 1956 with the development of the Latex Agglutination Test (Singer and Plotz, 1956). Recently a significant number of additional applications of polymer particles in the biomedical field have emerged. These applications exploit advances in polymer chemistry in combination with new developments in the field of biotechnology. Several of these applications are well underway, whereas others are at an early developmental stage. Several of these applications are described in the following paragraphs of this review, with emphasis on the use of microspheres in cell separation.

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References

  1. Antoine JC; Ternyac T; Rodisgot M and Auremeas S: Lymphoid cell fractionation on magnetic polyacrylamide-aganese beads. Immunochemistry 15:443, (1978).

    Article  CAS  Google Scholar 

  2. Applebaum FR; Herzig GP and Ziegler JL: Successful engraftment of cryopreserved autologous bone marrow in patients with malignant lymphoma. Blood 52:98, (1978a).

    Google Scholar 

  3. Applebaum FR; Diesseroth AB and Graw GR Jr.: Prolonged complete remission following high dose chemotherapy of Burkitt’s lymphoma in relapse. Cancer 41:1059, (1978b).

    Article  Google Scholar 

  4. Ash RC; Serwint M; Doukas M; Romond E; Bradley P; Metcalf M; Marshall E; Geil J; Greenwood M; MacDonald JS and Thompson JS: Marrow T cell depletion with anti-human T cell antibodies is effective for graft versus host disease (GvHD) prophylaxis in human allogeneic marrow transplantation. Blood 64(supp1-1):744 (1984).

    Google Scholar 

  5. Bartlett JM; Richardson RC; Elliot GS; Blevins WE; Janas W; Hale JR and Silver RL: Localization of magnetic microspheres in 36 canine osteogenic sarcomas. In: Microspheres and Drug Therapy. Pharmaceutical, Immunological and Medical Aspects, Davis SS; Illum L; McVie JG and Tomlinson E (eds) Elsevier, Amsterdam, p. 413 (1984).

    Google Scholar 

  6. Blacklock HA; Prentice HG and Gilmore M: Attempts at T cell depletion using OKT3 and rabbit complement to prevent GvHD in allogeneic BMT, Exp. Hemarol., 11 (suppl 13):37, (1983).

    Google Scholar 

  7. Bonnefoy JY; Banchereau J; Aubry JP and Wijdenes J: A flow cytometric micromethod for the detection of Fc epsilon receptors and IgE binding factors using fluorescent microspheres. J. Immunol. Meth. 88:25, (1986).

    Article  CAS  Google Scholar 

  8. Brasseur F; Couvreur P; Kante B; Deckers-Passau L; Roland M; Deckers C and Speiser P: Actinomycin D absorbed on polymethyl-cyanoacrylate nanoparticles: increased efficiency against an experimental tumor. Eur. J. Cancer 16:1441, (1980).

    CAS  Google Scholar 

  9. Buckner CD; Storb R and Dillingham LA: Low temperature preservation of monkey marrow in dimethylsulfoxide. Cryobiology 7: 136, (1970).

    Article  CAS  Google Scholar 

  10. Burdine JA; Sonnemaker RE; Ryder LA and Spjut HJ: Perfusion studies with technetium-99m human albumin microspheres. Radiology 95:101, 1970.

    CAS  Google Scholar 

  11. Burger JJ; Tomlinson E; DeRoo JE and Palmer J: Technetium-99m labeling of albumin microspheres intended for drug targeting. In: Drug and Enzyme Targeting, Widder KJ and Green R (eds) Meth. Enzymol. 112:43, (1985).

    Google Scholar 

  12. Carbone AM; Ovadia H and Paterson PY: Role of macrophage myelin basic protein interaction in the induction of experimental allergic-encephalomyelitis in Lewis Rats. J. Immunol. 131:1263, (1983).

    CAS  Google Scholar 

  13. Cowsar DR; Tice TR; Gilley RM and English JP: Poly(lactide-coglycolide) microcapsules for controlled release of steroids. In: Drug and Enzyme Targeting, Widder KJ and Green R (eds), Meth. Enzymol. 112:101, (1985).

    Google Scholar 

  14. Danielson H; Funderud S; Nustad K; Reith A and Ugelstad J: The interaction between cell surface antigens and antibodies bound to monodisperse polymer particles in normal and malignant cells. Scand. J. Immunol. 24:179, 1986.

    Google Scholar 

  15. Davis SS; Illum L; McVie JG and Tomlinson E (eds): Microspheres and Drug Therapy. Pharmaceutical, Immunological and Medical Aspects, Elsevier, Amsterdam, New York, pp. 448, (1984).

    Google Scholar 

  16. Deeg HJ; Storb R; Thomas ED et al: Marrow transplantation for acute, nonlymphoblastic leukaemia in first remission: Preliminary results of a randomized trial comparing cyclosporin and methotrexate for the prophylaxis of graft-versus-host disease. Transplant. Proc. 15:1385, (1983).

    Google Scholar 

  17. Dicke KA; Van Hooft J and Van Bekkum DW: The selective elimination of immunologically competent cells from bone marrow and lymphatic cell mixtures. Transplantation 6:562, (1968).

    Article  Google Scholar 

  18. Dicke KA; McCredie KB; Spitzer G et al: Autologous marrow transplantation in patients with adult acute leukemia. Transplantation 26:169, (1978).

    Article  CAS  Google Scholar 

  19. Dicke KA; Zander A; Spitzer G et al: Autologous bone marrow transplantation in adult leukemia in relapse. Lancet 1: 514, (1979).

    Article  CAS  Google Scholar 

  20. Dicke KA; Jagnnath S; Spitzer G; Poynton C; Zander A; Vellekoop L; Reading C; Jehn UW and Tindle S: The role of autologous bone marrow transplantation in various malignancies. Sem. Hematol. 21:109, (1984).

    CAS  Google Scholar 

  21. Diesseroth A and Abrams RA: The role of autologous stem cell reconstitution in intensive therapy for resistant neoplasms. Cancer Treat. Rep. 63:461, (1979).

    Google Scholar 

  22. Edman P and Sjoholm I: Acrylic microspheres in vivo. II. The effect in rat of L-asparaginase given in microparticles of polyacrylamide. J. Pharmacol. Exp. Ther. 211:663, (1979).

    CAS  Google Scholar 

  23. Edman P; Ekman B and Sjoholm I: Immobilization of proteins in microspheres of biodegradable polyacryldextran. J. Pharm. Sci. 69: 838, (1980).

    Article  CAS  Google Scholar 

  24. Edman P and Sjoholm I: Acrylic microspheres in vivo VI: antitumor effect of microparticles with immobilized L-asparaginase against 6C3HED lymphoma. J. Pharm. Sci. 72:654, (1983).

    Article  CAS  Google Scholar 

  25. Engvall E and Perlmann P: ELISA. III. Quantitation of specific antibodies by enzyme linked anti immunoglobulin in antigen coated tubes. J. Immunol. 109, 129, (1972).

    CAS  Google Scholar 

  26. Epstein RB; Storb R; Clift RA: Autologous bone marrow grafts in dogs treated with lethal doses of cyclophosphamide. Cancer Res. 29: 1072, (1969).

    CAS  Google Scholar 

  27. Etani H; Kimura K; Yoneda S; Tsuda Y; Nakamura M; Kataoka K; Iwata Y and Abe H: Demonstrating patency of STA-MCA anastomosis with Tc-99m albumin microspheres. J. Nucl. Med. 24:136, (1983).

    CAS  Google Scholar 

  28. Evans RL: U.S. Patent, 3,663,685, (1972a).

    Google Scholar 

  29. Evans RL: U.S. Patent, 3,663,687, (1972b).

    Google Scholar 

  30. Filipovich AH; Youle RJ; Neville Jr. DM; Vallera DA; Quinones RR and Kersey JH: Ex-vivo treatment of donor bone marrow with anti-T-cell immunotoxins for prevention of graft-versus-host disease. Lancet 1:469, (1984).

    Article  CAS  Google Scholar 

  31. Freeman AI and Mayhew E: Targeted drug delivery. Cancer 58:573, (1986).

    Article  CAS  Google Scholar 

  32. Frei E III; Canellos GP: Dose: a critical factor in cancer chemotherapy. Am. J. Med. 69:586, (1980).

    Google Scholar 

  33. Gale RP; Kersey JH; Bortin MM; Dicke KA; Good RA and Zwaan FE: Bone marrow transplantation for acute lymphoblastic leukaemia. Lancet 11:639, (1983).

    Google Scholar 

  34. Gaudernack G; Leivestad T; Qvigstad E; Ugelstad J and Thorsby E: Isolation of functionally active T cell subsets by monoclonal antibodies conjugated to monodisperse magnetic microspheres M-450. Scand. J. Immunol. 22:444, (1985).

    Google Scholar 

  35. Gaudernack G; Leivestad T; Ugelstad J; Thorsby E: Isolation of pure functionally active CD8+ T cells. Positive selection with monoclonal antibodies directly conjugated to monosized magnetic microspheres. J. Immunol. Meth. 90:179, (1986).

    Article  CAS  Google Scholar 

  36. Goldberg EP; Iwata H and Longo W: Hydrophilic albumin and dextran ion-exchanged microspheres for localized chemotherapy. In: Microspheres and Drug Therapy. Pharmaceutical, Immunological and Medical Aspects, Davis, SS; Illum JG; McVie JG and Tomlinson E (eds): Elsevier, Amsterdam, p.309, (1984).

    Google Scholar 

  37. Gordon IL; Dreyer WJ; Yen RCK and Rembaum A: Light microscope identification of murine B and T cells by means of functional polymeric microspheres. Cellular Immunology 28:307, (1977).

    Article  CAS  Google Scholar 

  38. Graze PR and Gale RP: Autotransplantation for leukemia and solid tumors. Transplant. Proc. 10:177, (1978).

    CAS  Google Scholar 

  39. Graze RP; Wells JR and Ho W: Successful engraftment of cryopreserved autologous bone marrow stem cells in man. Transplantation 27:142, (1979).

    Article  CAS  Google Scholar 

  40. Gribnau TC; Leuvering TH; van Hell H: Particle-labelled immunoassays: a review. J. Chromatogr. 376:175, (1986).

    Article  CAS  Google Scholar 

  41. Guesdon JL; Thierry R and Avrameas S: Magnetic enzyme immunoassay for measuring human IgE. J. Allergy Clin. Immunol. 61:23, (1978).

    Article  CAS  Google Scholar 

  42. Heymer B; Schachenmayer W; Buttmann B; Spank R; Haterkamp O and Schmidt W: A latex agglutination test for measuring antibodies to streptococcal micropeptides. J. Immunol. 11:478, (1973).

    Google Scholar 

  43. Higgins TJ; O’Neill HC and Parrish CRA: A sensitive and quantitative fluorescence assay for cell surface antigens. J. Immunol. Meth. 47:275, (1981).

    Article  CAS  Google Scholar 

  44. Hof RP: Measuring regional blood flow with tracer microspheres: a method, its problems and its application. Blood Circulation 21:29, (1982).

    CAS  Google Scholar 

  45. Hoffbrand BI and Forsyth RP: Validity studies of the radioactive microsphere method for the study of the distribution of cardiac output, organ blood flow and resistance in the conscious rhesus monkey. Cardiovascular Research 3:426, (1969).

    Article  CAS  Google Scholar 

  46. Hollenberg NK: The Renal Circulation. In: The Peripheral Circulation, Zelis R; (ed) Grune Stratton, New York, (1975).

    Google Scholar 

  47. Hsieh DS; Langer R and Folkman J: Magnetic modulation of release of macromolecules from polymers. PNAS 78:1863, (1981).

    Article  CAS  Google Scholar 

  48. Hunter WM: Radioimmunoassay. Handbook of Experimental Immunology, Weir DM (ed), Blackwell Scientific Publications, p. 14.1, (1978).

    Google Scholar 

  49. Illum L and Jones PDE: Attachment of Monoclonal Antibodies to Microspheres. In: Drug and Enzyme Targeting, Widder KJ and Green R (eds): Heth. Enzymol. 112:67, (1985).

    Google Scholar 

  50. Jolley ME; Wang CH; Ekenberg SJ; Zuelke MS and Kelso DM: Particle concentration fluorescence immunoassay (PCFIA): A new rapid immunoassay technique with high sensitivity. J. Immunol. Meth. 67:21, (1984).

    Article  CAS  Google Scholar 

  51. Kandzia J; Anderson MJD and Mueller-Ruchholtz W: Cell separation by antibody coupled magnetic microsphere and their application in conjunction with monoclonal HLA antibodies. J. Cancer Res. Clin. Oncol. 101:165, (1981).

    Article  CAS  Google Scholar 

  52. Kandzia J; Scholz W; Anderson MJD and Mueller-Ruchholtz W: Magnetic albumin protein A immunomicrospheres. I. Preparation, antibody binding capacity and chemical stability. J. Immunol. Meth. 75:413, (1985).

    Google Scholar 

  53. Kaplan MR; Calef E; Bercovici T and Gitler C: The selective detection of cell surface determinants by means of antibodies and acetylated avidin attached to highly fluorescent polymer microspheres. Biochim. Biophys. Acta. 728:112, (1983).

    Article  CAS  Google Scholar 

  54. Kato T and Nemoto R: Microencapsulation of mitomycin C intra-arterial infusion chemotherapy. Proc. Jpn. Acad. Ser. B 54:413, (1978).

    Article  CAS  Google Scholar 

  55. Kato T; Nemoto R; Mori H and Kumagai I: Sustained-release properties of microencapsulated mitomycin C with ethylcellulose infused into the renal artery of the dog kidney. Cancer 46:14, (1980).

    Article  CAS  Google Scholar 

  56. Kato T; Nemoto R; Mori H; Takahashi M; Harada M: Arterial chemoembolization with mitomycin C microcapsules in the treatment of primary or secondary carcinoma of the kidney, liver, bone and intrapelvic organs. Cancer 48:674, (1981).

    Article  CAS  Google Scholar 

  57. Kato T; Unno K and Goto A: Ethylcellulose microcapsules for selective drug delivery. In: Drug and Enzyme Targeting, Widder KJ and Green R (eds). Meth. Enzymol 112:139, (1985).

    Google Scholar 

  58. Kemshead JT; Treleaven J; Gibson F; Ugelstad J; Rembaum A and Philip T: Monoclonal antibodies and magnetic microspheres used for the depletion of malignant cells from bone marrow. In “Autologous bone marrow transplantation: Proceedings of the First International Symposium” Dicke KA; Sptzer G and Zander A (eds.) U. of Texas Press, Houston, TX, p.409, (1985).

    Google Scholar 

  59. Kernan NA; Burns MJ; Collins NH; O’Reilly RJ and Dupont B: Limiting dilution microculture assay for quantitation of T lymphocytes in bone marrow. Transpl. Proc. 17:437, (1985).

    Google Scholar 

  60. Khalafalla SE and Reimers GW: Preparation of dilution-stable aqueous magnetic fluids. IEEE Trans. Magnetics 16:178, (1980).

    Article  Google Scholar 

  61. Kitchell JP and Wise DL: Poly(lactic/glycolic acid) biodegradable drug-polymer matrix systems. In: Drug and Enzyme Targeting, Widder KJ and Green R (eds.) Meth. Enzymol 112:436, (1985).

    Google Scholar 

  62. Korbling M; Hess AD; Tutschuka PJ; Kaiser H; Colvin MO and Santos GW: 4-Hydroperoxycyclophosphamide: a model for eliminating residual tumour cells and T-lymphocytes from the bone marrow. Br. J. Haematol. 52:89, (1982).

    Article  CAS  Google Scholar 

  63. Korngold R and Sprent J: Surface markers of T cells causing lethal graft-versus-host disease in mice, Recent Advances in Bone Marrow Transplantation: Gale RP and Liss AR (eds), New York, p.199, (1983).

    Google Scholar 

  64. Kreuter J and Hartmann HR: Comparative study on the cytostatic effects and the tissue distribution of 5-fluorouracil in a free form and bound to polybutylcyanoacrylate nanoparticles in sarcoma 180 bearing mice. Oncology, 40:363, (1983).

    Article  CAS  Google Scholar 

  65. Kreuter J: Evaluation of nanoparticles as drug-delivery systems. II: Comparison of the body distribution of nanoparticles with the body distribution of micro spheres (diameter greater than 1 micron), liposomes and emulsions. Pharm. Acta. Helv. 58:217, (1983).

    CAS  Google Scholar 

  66. Kreuter J: Poly(alkyl acrylate) Nanoparticles. In: Drug and Enzyme Targeting, Widder KJ and Green R (eds). Meth. Enzymol. 112:129, (1985).

    Google Scholar 

  67. Kronick PL; Campbell G and Joseph K: Magnetic microspheres prepared by redox polymerization used in a cell separation based on gangliosides. Science 200:1074, (1978).

    Article  CAS  Google Scholar 

  68. Kronick PL: Magnetic microspheres in cell separation. Methods Cell Separation 3:115, (1980).

    Google Scholar 

  69. Laakso T; Anderson J; Artursson P; Edman P; Sjoholm I: Acrylic microspheres in vivo. X. Elimination of circulating cells by active targeting using specific monoclonal antibodies bound to microparticles. Life Sci. 28:183, (1986).

    Article  Google Scholar 

  70. Laubins JD and Ross GD: Assay of membrane complement receptors (CR1 and CR2) with C3b — and (3d-coated florescence microspheres. J. Immunol. 128:186 (1982).

    Google Scholar 

  71. Langer R and Folkman: J. Polymers for the sustained release of proteins and other macromolecules. Nature 263:797, (1976).

    Article  CAS  Google Scholar 

  72. Langer R; Brown L and Edelman E: Controlled Release and Magnetically Modulated Release Systems for Macromolecules. In: Drug and Enzyme Targeting, Widder KJ and Green R (eds) Meth. Enzymol. 112:399, (1985).

    Google Scholar 

  73. Lea T; Vartdal F; Davies C and Ugelstad J: Magnetic monosized polymer particles for fast and specific fractionation of human mononuclear cells. Scan. J. Immunol. 22:207, (1985).

    CAS  Google Scholar 

  74. Lichtenstein B: U.S. Patent No. 3,709,791 (1973).

    Google Scholar 

  75. Lim F (ed): Biomedical Applications in microencapsulation, CRC Press, Boca Raton, (1984).

    Google Scholar 

  76. Lindberg B; Lote K and Teder H: Biodegradable starch and microspheres: A new medical tool. In: Microspheres and Drug Therapy, Pharmaceutical, Immunological and Medical Aspects: Davis SS; Illum JG; McVie and Tomlinson E (eds) Elsevier, Amsterdam. p. 153, (1984).

    Google Scholar 

  77. Ljungstedt I; Ekman B and Sjoholm I: Detection and separation of lymphocytes with specific surface receptors by using microparticles. Biochem. J. 170:161, (1978).

    CAS  Google Scholar 

  78. Longo WE and Goldberg EP: Hydrophilic albumin microspheres. In: Drug and Enzyme Targeting, Widder KJ and Green R (eds): Meth. Enzymol. 112:18, (1985).

    Google Scholar 

  79. Malin S and Edwards: J. Detection of hepatitis associated antigen by latex agglutination. Nature (Lond.) 235:182, (1972).

    CAS  Google Scholar 

  80. Marcus L; Offarim M and Margel S: A new immunoadsorbent for hemoperfusion: agarose-polyanolein microspheres beads. I. In vitro studies. Biomater. Med. Devices Artif. Organs. 10:157 (1982).

    CAS  Google Scholar 

  81. Marcus L; Mashiah A; Offarim M and Margel S: Extracorporeal removal of specific antibodies by hemoperfusion through immunosorbent agarose-polyacrolein microsphere beads: removal of antibovine serum albumin in animals. J. Biomed. Mater. Res. 18:1153, (1984).

    Article  CAS  Google Scholar 

  82. Marcus L; Margel S; Savin H; Offarim H and Ravid M: Therapy of digoxin intoxication in dogs by specific hemoperfusion through agarose polyacrolein microsphere beads-antidigoxin antibodies. Am. Heart J. 110:30, (1985).

    Article  CAS  Google Scholar 

  83. Margel S and Offarim M: Novel effective immunoadsorbents based on agarose-polyaldehyde microsphere beads: synthesis and affinity chromatography. Anal. Biochem. 128:342, (1983).

    Article  CAS  Google Scholar 

  84. Margel S and Marcus L: Specific hemoperfusion through agarose acrobeads. Appl. Biochem. Biotechnol. 12:37, (1986).

    Article  CAS  Google Scholar 

  85. Margel S; Zisblatt S and Rembaum A: Polyglutaraldehyde: A new reagent for coupling proteins to microspheres and for labeling cell surface receptors. II. Simplied labeling methods by means of non-magnetic and magnetic polyglutaraldehyde microspheres. J. Immunol. Meth. 28:341, (1979).

    Article  CAS  Google Scholar 

  86. Margel S; Beitler U and Ofarim M: Polyacrolein microspheres as a new tool in cell biology. J. Cell Sci. 56:157, (1982).

    CAS  Google Scholar 

  87. Margel S; Ofarim M; Eshar Z: Cell fractionation with affinity ligands conjugated to agarose-polyacrolein microsphere beads. J. Cell Sci. 62:149, (1983).

    CAS  Google Scholar 

  88. Margel S; Marcus L; Savin H; Offarim H and Mashiah A: Specific removal of digoxin by hemoperfusion through agarose polyacrolein microsphere polyacrolein microsphere beads and antidigoxin antibodies. Biomater. Hed. Devices Artif. Organs 12:25, (1984).

    CAS  Google Scholar 

  89. Melville D; Paul F and Roath S: Direct magnetic separation of red cells from whole blood. Nature 255:706, (1975).

    Article  CAS  Google Scholar 

  90. Merivuori H; DeLa-Chapella A and Schroder J: Cell labeling and separation with polyglutaraldehyde microspheres. Exp. Cell. Res. 130:464, (1980).

    Article  CAS  Google Scholar 

  91. Milgram F and Goldstein R: Agglutination of sensitized red blood cells by latex particles. Vox. Sang. 7:86, (1962).

    Article  Google Scholar 

  92. Miller AM; McMillan L; Hannan WJ; Emmett PC and Aitken RJ: The preparation of dry, monodisperse microspheres of 99m Tc-albumin for lung ventilation imaging. Int. J. Appl. Radiat. Isot. 33: 1423, (1982).

    Article  Google Scholar 

  93. Miller RA; Brady JM and Cutright DE: Degradation rates of oral resorbable implants (polylactates and polyglycolates): rate modification with changes in PLA/PGA copolymer ratios. J. Biomed. Mat. Res. 11:711, (1977).

    Article  CAS  Google Scholar 

  94. Mirro J; Schwartz JF and Civin CI: Simultaneous analysis of cell surface antigen and cell morphology using monoclonal antibodies conjugated to fluorescent microspheres. J. Immunol. Meth. 47:39, (1981).

    Article  Google Scholar 

  95. Mizushima Y; Shoji Y; Kato T; Fukushima M and Kurozumi S: Use of lipid microspheres as a drug carrier for antitumor drugs. J. Pharm. Pharmacol. 38:132, (1986).

    Article  CAS  Google Scholar 

  96. Molday RS; Dryer WJ; Rembaum A and Yen RCK: Latex spheres as marker for studies of cell surface receptors by scanning electron microscopy. Nature 249:81, (1974).

    Article  CAS  Google Scholar 

  97. Molday RS; Yen RCK and Rembaum A: Application of magnetic microspheres in labeling and separation of cells. Nature 2268:437, (1977).

    Article  Google Scholar 

  98. Molday RS and MacKenzie KD: Immunospecific ferromagnetic iron-dextran reagents for the labeling and magnetic separation of cells. J. Immunol. Meth. 52:353, (1982).

    Article  CAS  Google Scholar 

  99. Molday RS: Cell labeling and separation using immunospecific microspheres. In: Cell Separation, Methods and Selected Applications, 3, Pretlow II, TG and Pretlow TP (eds); Academic Press, NY, p.237, (1984).

    Google Scholar 

  100. Molday RS; Dreyer WJ; Rembaum A and Yen RCK: New immunolatex spheres: Visual markers of antigens on lymphocytes for scanning electron microscopy. J. Cell Biol. 164:75, (1975).

    Article  Google Scholar 

  101. Morimoto Y; Sugibayashi K and Akimoto M: Magnetic guidance of Ferro-colloid-entrapped emulsion for site-specific drug delivery. Chem. Pharm. Bull. 31:279, (1983).

    Article  CAS  Google Scholar 

  102. Morris RM; Poore GA; Howard DP and Sefranka JA: Selective targeting of magnetic albumin microspheres containing vindesine sulfate: Total remission in Yoshida sarcoma-bearing rats. In: Microspheres and Drug Therapy. Pharmaceutical, Immunological and Medical Aspects, Davis SS; Illum L; McVie JG and Tomlinson E (eds): Elsevier, Amsterdam, p.439, (1984).

    Google Scholar 

  103. Mosbach K and Schroder UI: Preparation and application of magnetic polymers for targeting of drugs. FEBS Lett. 102:112, (1979).

    Article  CAS  Google Scholar 

  104. Murray J; Brown L and Langer R: Controlled release of microquantitites of macromolecules. Cancer Drug Delivery, 1:119, (1984).

    Article  CAS  Google Scholar 

  105. Nilsson K and Mosbach K: p-Toluensulfonyl chloride as an activating agent of agarose for the preparation of immobilized affinity ligands and proteins. Eur. J. Biochem. 112:397, (1980).

    Article  CAS  Google Scholar 

  106. Nustad K; Johansen L; Ugelstad J; Ellingsen T and Berge A: Hydrophilic monodisperse particles as solid-phase material in immunoassays: Comparison of shell-and-core particles with compact particles. Eur. Surg. Res. 16:Suppl 2, 80, (1984 ).

    Article  CAS  Google Scholar 

  107. Nustad K; Danielsen H; Reith A; Funderud S; Lea T; Vartdal F and Ugelstad J. Monodisperse polymer particles in immunoassays and cell separation, in Microspheres: Medical and Biological Applications, Rembaum A and Tokes ZA (eds), CRC Publications, in press (1987).

    Google Scholar 

  108. Obertenffer JA: High gradient magnetic separation. IEEE Trans. Magnetics 9:303, (1973).

    Article  Google Scholar 

  109. Oder RR: High gradient magnetic separation theory and applications. IEEE Trans. Magnetics 12:428, (1976).

    Article  Google Scholar 

  110. Ohman M; Garovoy MR; Widder KJ and Streigel JA: Depletion of T lymphocytes from peripheral blood using monoclonal antibodies and protein magnetic microspheres. Hum. Immunol. 14:128, (1985).

    Article  Google Scholar 

  111. Ohnishi K; Tsuchiya S and Nakayma T: Arterial chemoembolization of hepatocellular carcinoma with mitomycin C microcapsules. Radiology 152:51, (1984).

    CAS  Google Scholar 

  112. Oonishi T and Uyesaka N: A new standard fluorescence microsphere for quantitative flow cytometry. J. Immunol. Meth. 83:1143, (1985).

    Google Scholar 

  113. Onoe K; Fernandes G and Good RA: Humoral and cell-mediated immune responses in fully allogeneic bone marrow chimeras in mice. J. Exp. Med. 151:115, (1980).

    Article  CAS  Google Scholar 

  114. Ovadia H; Carbone AM and Paterson PY: Albumin magnetic microspheres a novel carrier for myelin basic protein. J. Immunol. Meth. 53:109, (1982).

    Article  CAS  Google Scholar 

  115. Ovadia H; Paterson PY and Hale JR: Magnetic microspheres as drug carriers: Factors influencing localization at different anatomical sites in rats. Isr. J. Med. Sci. 19:631, (1983).

    CAS  Google Scholar 

  116. Owen CS: High gradient magnetic separation of erythrocytes. Biophys. J. 22:171, (1978).

    Article  CAS  Google Scholar 

  117. Owen CS: Magnetic cell sorting. In: Cell Separation Methods and Selected Applications. Pretlow II, TG and Pretlow TP (eds) Academic Press, NY, 2, p.127, (1983).

    Google Scholar 

  118. Owen CS: Magnetic sorting of leukocytes. Cell Bioph. 8: 287 (1986).

    CAS  Google Scholar 

  119. Owen CS and Liberti PA: Magnetic-Protein conjugates for the separation of cells by high gradient magnetic filtration. In: Cell Separation: Methods and Selected Applications. Pretlow II, TG and Pretlow TP (eds) Academic Press, NY, 4 in press (1986).

    Google Scholar 

  120. Owen CS and Sykes NL: Magnetic labeling and cell sorting. J. Immunol. Meth. 73:41, (1984).

    Article  CAS  Google Scholar 

  121. Phibbs RH et al. Rheology of micro spheres injected into circulation of rabbits. Nature 216:1339, (1967).

    Article  CAS  Google Scholar 

  122. Platsoucas CD and Catsimpoolas N: Biological methods for the separation of lymphoid cells. Meth. Cell Separation 3: 157, (1980).

    Google Scholar 

  123. Platsoucas CD and Good RA: Inhibition of specific cell mediated cytotoxicity by monoclonal antibodies to human T-cell antigens. Proc. Natl. Acad. Sci. USA 78:4500, (1981).

    Article  CAS  Google Scholar 

  124. Platsoucas CD; Good RA and Gupta S: Separation of human lymphocyte subpopulations by density gradient electrophoresis. I. Different mobilities of T (Tmu, Talpha) and B lymphocytes from human tonsils. Cell. Immunol. 51:238, (1980).

    Article  CAS  Google Scholar 

  125. Platsoucas CD; Beck JD; Kapoor N; Good RA and Gupta S: Separation of human bone marrow cell populations by density gradient electrophoresis: Differential mobilities of myeloid (CFU-C), monocytoid, and lymphoid cells. Cellular Immunol. 59:345, (1981).

    Article  CAS  Google Scholar 

  126. Platsoucas CD; Chae F; Collins N; Kerman N; Laver J; Ellinger T; Steristad P; Bjorgum J; Rembaum A; Good RA; O’Reilly R and Ugelstad J: The use of magnetic monosized polymer particles for the removal of T cells from human bone marrow cell suspensions. Microspheres: Medical and Biological Applications: Rembaum A and Tokes Z (eds) CRC Press, Inc., Boca Raton, Florida, in press, (1987a).

    Google Scholar 

  127. Platsoucas CD; Kerman N; Collins N; Chae F; Ugelstad J and O’Reilly R: Depletion of T cells from human bone marrow cell suspension using the M-450 magnetic monosized polymer particles and anti-T cell monoclonal antibodies, Submitted (1987b).

    Google Scholar 

  128. Powles RL; Clink H; Sloane J; Barrett AJ; Kay HE and McElwain TJ: Cyclosporin A for the treatment of graft-versus-host disease in man. Lancet 2:1327, (1978).

    Article  CAS  Google Scholar 

  129. Poynton CH; Dicke KA; Culbert S; Frankel L; Jagannath S and Reading CL: Immunomagnetic removal of CALLA positive cells from human bone marrow. Lancet 1:524, (1983).

    Article  CAS  Google Scholar 

  130. Poynton CH; Reading CL and Dicke KA: Colloidal immunomagnetic fluids for cells separation. In “Autologous bone marrow transplantation; Proceedings of the Fifth International Symposium”. Dicke KA; Spitzer G and Zander A (ed), U. of Texas Press, Houston, TX p.433, (1985).

    Google Scholar 

  131. Prentice HG; Janossy G; Price-Jones L; Trejdosiewicz LK; Panjwani D; Graphakos S; Ivory K; Blacklock, HA; Gilmore ML; Tidman N; Skeggs DBL; Ball S; Patterson J and Hoffbrand AV: Depletion of T lymphocytes in donor marrow prevents significant graft-versus-host disease in matched allogeneic leukaemic marrow transplant recipients. Lancet 1:472, (1984).

    Article  CAS  Google Scholar 

  132. Ramsey NKC; Kersey JH; Robison LL et al: Prevention of acute graft-versus-host disease: a randomized study demonstrating the influence of treatment regimen and age. N. Engl. J. Med. 306:392 (1982).

    Article  Google Scholar 

  133. Reisner Y; Itziscovitch L; Meshorer A and Sharon N: Hemopoietic stem cell transplantation using mouse bone marrow and spleen cells fractionated by lectins. Proc. Natl. Acad. Sci. USA. 75: 2933, (1978).

    Article  CAS  Google Scholar 

  134. Reisner Y; Kapoor N; O’Reilly RJ and Good RA: Allogeneic bone marrow transplantation using stem cells fractionated by lectins: VI. In vitro analysis of human and monkey bone marrow cells fractionated by sheep red blood cells and soybean agglutinin. Lancet 2:1320, (1980).

    Article  CAS  Google Scholar 

  135. Reisner Y; Kapoor N; Kirkpatrick D; Pollack M; Dupont B; Good RA and O’Reilly RJ: Transplantation for acute leukaemia with HLAA and B non-identical parental marrow cells fractionated with soybean agglutinin and sheep red blood cells. Lancet 2: 327, (1981).

    Article  CAS  Google Scholar 

  136. Reisner Y; Kapoor N; Kirkpatrick D; Cunningham-Rundles S; Dupont B; Hodes MZ; Good RA and O’Reilly RJ: Transplantation for severe combined immunodeficiency with HLA-A, B, D, DR incompatible parental marrow cells fractionated by soybean agglutinin and sheep red blood cells. Blood 61:341, (1983).

    CAS  Google Scholar 

  137. Rembaum A, Margel S and Levy J: Polyglutaraldehyde: A new reagent for coupling proteins to microspheres and for labeling cell surface receptors. J. Immunol. Meth. 24:239 (1978).

    Article  CAS  Google Scholar 

  138. Rembaum A; Yen RCK; Kempner DH and Ugelstad J: Cell labeling and magnetic separation by means of immunoreagents based on polyacrolein microspheres. J. Immunol. Meth. 52:341, (1982).

    Article  CAS  Google Scholar 

  139. Reske SN; Vyska K and Feinendegen LE: In vivo assessment of phagocytic properties of Kupffer cells. J. Nucl. Med. 22:405, (1981).

    CAS  Google Scholar 

  140. Reynolds CP; Seeger RC; Vo DD; Ugelstad J and Wells J (eds) Vince UA; Spitzer G and Zander A: Purging of bone marrow with immunomagnetic beads: Studies with neuroblastoma as a model system. In: Autologous Bone Harrow Transplantations”, Proceedings of the Fifth International Symposium, U. of Texas Press, Houston, TX, p. 439 (1985).

    Google Scholar 

  141. Ritz J; Bast RC and Clavell LA: Autologous bone-marrow translantation in CALLA-positive acute lymphoblastic leukaemia after in vitro treatment with J5 monoclonal antibody and complement. Lancet 11:60, (1982).

    Article  Google Scholar 

  142. Rodt H; Kolb HJ; Netzel B; et al. Effect of anti-T cell globulin on GvHD in leukaemic patients treated with BMT. Transplant. Proc. 13:257, (1981).

    CAS  Google Scholar 

  143. Rodt H; Thierfelder S and Eulitz M: Anti-lymphocytic antibodies and marrow transplantation. III. Effect of heterologous anti-brain antibodies on acute secondary disease in mice. Eur. J. Immunol. 4:25, (1974).

    Article  Google Scholar 

  144. Rose NR and Friedman H: Manual of Clinical Immunology, Second Edition, American Society for Microbiology, Washington, D.C., pp.1105, (1980).

    Google Scholar 

  145. Ross GD and Lambris JD: Identification of a C3bi-specific membrane complement receptor that is expressed on lymphocytes, monocytes, neutrophils and erythrocytes. J. Exp. Med. 1155:96, (1982).

    Article  Google Scholar 

  146. Rous P and Beard JW: Selection with the magnet and cultivation of reticuloendothelial cells (Kupffer cells). J. Exp. Med. 59:577, (1934).

    Article  CAS  Google Scholar 

  147. Russell GFJ: Starch microspheres as drug delivery systems. Pharm. Int. 4:260, (1983).

    CAS  Google Scholar 

  148. Scheffel V; Rhodes BA; Natarjan TK and Wagner HN: Albumin microspheres for study of the reticuloendothelial system. J. Nuclear Med. 13:498 (1972).

    CAS  Google Scholar 

  149. Schroder U: Crystallized Carbohydrate Spheres for Slow Release and Targeting. Meth. Enzymol. 112:1116, (1985).

    Google Scholar 

  150. Seeger RC; Reynolds PD; Vo J; Ugelstad J and Wells J: Depletion of neuroblastoma cells from bone marrow with monoclonal antibodies and magnetic immunobeads. In “Advances in Neuroblastoma Research”, Evans AE; D’Angio G and Seeger RC (eds): Alan R. Liss, Inc. NY p.443, (1985).

    Google Scholar 

  151. Senyei A; Widder K and Czerlinksi G: Magnetic guidance of drug-carrying microspheres. J. App. Phys. 49:3578, (1978).

    Article  CAS  Google Scholar 

  152. Senyei AE; Driscoll CF and Widder KJ: Biophysical Drug Targeting: Magnetically Responsive Albumin Microspheres. In: Drug and Enzyme Targeting: Widder KJ and Green R (eds) Meth. Enzymol. 112:56, (1985).

    Google Scholar 

  153. Sharp TG; Sachs DH; Fauci AS; Messerschmidt GL and Rosenberg SA T cell depletion of human bone marrow using monoclonal antibody and complement-mediated lysis. Transplantation 35:112, (1983).

    Article  CAS  Google Scholar 

  154. Simonsen M: The impact on the developing embryo and newborn animal of adult homologous cells. Acta Path. Miocrobiol. Scand. 40: 480, (1957).

    CAS  Google Scholar 

  155. Singer JM and Plotz CM: The latex fixation test. I. Application to the serological diagnosis of rheumatoid arthritis. Am. J. Med. 21:888, (1956).

    Article  Google Scholar 

  156. Singer JM: The latex fixation test in rheumatic disease. A review. Am. J. Med. 31:766, (1961).

    Article  CAS  Google Scholar 

  157. Singer JM: The use of polystyrene latexes in Medicine, in: Future Directions in Polymer Colloids, El-Aasser MS and Fitch R (eds) Martinus-Nijhoff Publishing Company, Bordrecht, The Netherlands. This volume (1987).

    Google Scholar 

  158. Singh M; Silver RL; Lin C; Chowdhury L and Post M: Localization with magnetic microspheres reduces the systemic toxicity of doxorubicin. Orthop Trans 8:324, (1984).

    Google Scholar 

  159. Sjoholm I and Edman P: Acrylic microspheres in vivo. I. Distribution and elimination of polyacrylamide microparticles after intravenous and intraperitoneal injection in mouse and rat. J. Pharmacol. Exp. Ther. 211:656, (1979).

    CAS  Google Scholar 

  160. Sjoholm I and Edman P: The use of biocompatibile micropartic1es as carriers of enzymes and drugs in vivo. In: Microspheres and Drug Therapy. Pharmaceutical, Immunological and Medical Aspects, S. S. Davis, L. Illum, McVie JG and Tomlinson E (eds) Elsevier, Amsterdam, p.245, (1984).

    Google Scholar 

  161. Skipper HE; Schnabel FM, Jr; Wilcox WS: Experimental evaluation of potential anticancer agents. XIII. On the criteria and kinetics associated with “curability” of experimental leukemia. Cancer Chemother. Rep. 34:1, (1964).

    Google Scholar 

  162. Storb R; Prentice RL; Buckner CD; Clift RA; Appelbaum F; Deeg J; Doney K; Hansen RP; Mason M; Sanders JE; Singer J; Sullivan KM; Witherspoon RP and Thomas ED: Graft-versus-host disease and survival in patients with aplastic anemia treated by marrow grafts from HLA-identical siblings. Beneficial effect of a protective environment. N. Engl. J. Med. 308:302, (1983).

    Article  CAS  Google Scholar 

  163. Sugibayashi K; Morimoto Y; Nadai T; Kato Y; Hasegawa A; Arita T: Drug-carrier property of albumin microspheres in chemotherapy. II. Preparation and tissue distribution in mice of microsphere-entrapped 5-fluorouracil. Chem. Pharm. Bull 27:204, (1979).

    Article  CAS  Google Scholar 

  164. Sugibayashi K; Okumura M and Morimoto Y: Biomedical applications of magnetic fluids III. Antitumor effect of magnetic albumin microspheres-entrapped adriamycin on lung metastasis of AH-7974 in rats. Biomaterials 3:181, (1982).

    Article  CAS  Google Scholar 

  165. Terman DS; Young JB; Sheaver WT; Ayus C; Lehane D; Mattioli C; Espada R; Howell JF; Yamamoto T; Zaleski HI; Miller L; Frommer P; Feldman L; Henry JF; Tilquist R; Cook G and Daskal Y: Preliminary observations of the effects on breast adenocarcinoma of plasma perfused over immobilized protein A. N. Engl. J. Med. 305:1195, (1981).

    Article  CAS  Google Scholar 

  166. Terman DS; Yamamoto T; Mattioli M; Cook G; Tilquist R; Henry J; Poser R and Daskal Y: Extensive necrosis of spontaneous canine mammary adenocarcinoma after extracorporeal perfusion over Staphylococcus aureus Cowans I. Description of acute tumoricidal response: Morphologic, Histologic, Immunohistochemical, Immunologic, and Serologic Findings. J. Immunol. 124:795,1 (1980).

    CAS  Google Scholar 

  167. Theodorakis MC; Digenis GA; Beihn RM; Shambhu MB and DeLand FH: Rate and pattern of gastric emptying in humans using 99mTc-labeled triethylenetetraamine polystyrene resin. J. Pharmc. Sci. 69: 568, (1980).

    Article  CAS  Google Scholar 

  168. Thomas E; Storb R; Clift R; et al. Bone marrow transplantation. N. Engl. J. Med. 292:895, (1975).

    Article  CAS  Google Scholar 

  169. Thorpe PE; Mason DW; Brown ANF; Simmonds SJ; Ross WCJ; Cumber AJ and Forrester JA: Selective killing of malignant cells in leukemic rat bone marrow using an antibody-ricin conjugate. Nature 297:594, (1982).

    Article  CAS  Google Scholar 

  170. Tobias JS; Weiner RS, Griffiths CT; Richman CM; Panier LM and Yourvee RA: Cryopresened autologous marrow infusion following high dose cancer chemotherapy. Eur. J. Cancer, 13:269 (1977).

    CAS  Google Scholar 

  171. Tokes ZA; Rogers KE and Rembaum A: Synthesis of adriamycin-coupled polyglutaraldehyde microspheres and evaluation of their cytostatic activity. PNAS 79:2026, (1982).

    Article  CAS  Google Scholar 

  172. Tomaszekwski JE; Good DB and Zmijewski CM: Cell surface antigen identification by a modified fluorescein immunosphere method. Am. J. Clin. Pathol. 85:219, (1986).

    Google Scholar 

  173. Tomlinson E and Burger JJ: Incorporation of water-soluble drugs in albumin microspheres. In: Drug and Enzyme Targeting, Widder KJ and Green R (eds). Meth. Enzymol. 112:27, (1985).

    Google Scholar 

  174. Treleaven JG; Ugelstad J; Philip T; Gibson FM; Rembaum A; Caine GD and Kemshead JT: Removal of neuroblastoma cells from bone marrow with monoclonal antibodies conjugated to magnetic microspheres. Lancet 1:70, (1984).

    Article  CAS  Google Scholar 

  175. Tuma RF: The use of degradable starch microspheres for transient occlusion of blood flow and for drug targeting to selected tissues. In: Microspheres and Drug Therapy. Pharmaceutical, Immunological and Medical Aspects, Davis SS; Illum L; McVie JG and Tomlinson E (eds) Elsevier, Amsterdam, p.1 189, (1984).

    Google Scholar 

  176. Tyan M: Modification of severe graft-versus-host disease with antisera to the theta antigen or to whole serum. Transplantation 15:601, (1973).

    Article  CAS  Google Scholar 

  177. Ugelstad J; Kaggerud KH; Hansen FK et al: Absorption of low molecular weight compounds in aqueous dispersions of polymeroligomer particles: a two step swelling process of polymer particles giving an enormous increase in absorption capacity. Makromol Chem. 180:737, (1979).

    Article  CAS  Google Scholar 

  178. Ugelstad J; Mork PC; Kaggerud KH et al: Swelling of oligomer particles. New methods of preparation of emulsions and polymer dispersion. Advances in Colloid Interface Science 13:101, (1980).

    Article  CAS  Google Scholar 

  179. Ugelstad J; Soderberg L; Berge A and Bergstrom J: Monodisperse polymer particles-a step forward for chromatography. Nature 303:96, (1983).

    Article  Google Scholar 

  180. Ugelstad J; Berge A; Ellingsen T; Bjorun J; Schmid R; Stenstad P; Aume O; Nilsen TN; Funderud S and Nustad K: Biomedical applications of monodisperse magnetic polymer particles, In: Future Directions in Polymer Colloids, ElAasser MS and Fitch R (eds): Martinuns-Nijhoff Publishing Company, Dordrecht, The Netherlands. This volume (1987).

    Google Scholar 

  181. Vallera DA; Youle RJ; Neville DM and Kersey JH: Bone marrow transplantation across major histocompatibility barriers. V. Protection of mice from lethal graft-vs-host disease. Pretreatment of donor cells with monoclonal anti-Thy-1-2 coupled to the toxin ricin. J. Exp. Med. 155:949, (1982).

    Article  CAS  Google Scholar 

  182. Voller A; Bidwell D; Bartlett A: Double antibody sandwich method for detection and measurement of antigen. In: Rose NR; Friedman H (eds) Manual of Clinical Immunology, Chapter 29, Washington, D.C., American Society for Microbiology, (1980).

    Google Scholar 

  183. Walsh JH; Yalow R and Benson SA: Detection of Australia antigen and antibody by means of radioimmunoassay techniques. J. Infect. Dis. 121:550, (1970).

    Article  CAS  Google Scholar 

  184. Watson JHP: Magnetic filtration. J. Appl. Phys. 44:4209, (1973).

    Article  CAS  Google Scholar 

  185. Weiden PL and the Seattle Marrow Transplant Team. Graft-vs-host disease in allogeneic marrow transplantation. In: Gale RP; Fox CF (eds). Biology of bone marrow transplantation. New York, Academic Press, (1980).

    Google Scholar 

  186. Wells JR; Sullivan A and Cline MJ: A technique for the separation and cryopreservation of myeloid stem cells from human bone marrow. Cryobiology 16:201, (1979).

    Article  CAS  Google Scholar 

  187. Widder KJ; Senyei AE and Scarpelli DG: Magnetic microspheres: A model system for site specific drug delivery in vitro. Proc. Soc. Exp. Biol. Med. 58:141, (1978).

    Google Scholar 

  188. Widder KJ; Flauret G and Senyei A: Magnetic microspheres: Synthesis of a novel parenteral drug carrier. J. Pharm. Sci. 68: 79, (1979a).

    Article  CAS  Google Scholar 

  189. Widder KJ; Senyei AE; Ovadia H and Peterson PY: Magnetic protein A microspheres: A rapid method for cell separation. Clin. Immunol. Immunopathol. 14:395, (1979b).

    Article  CAS  Google Scholar 

  190. Widder KJ; Morris RK; Poore G; Howard Jr. DP and Senyei AE: Tumor remission in Yoshida Sarcoma bearing rats by selective targeting of magnetic albumin microspheres containing doxorubicin. Proc. Natl. Acad. Sci. USA 78:579, (1981a).

    Article  CAS  Google Scholar 

  191. Widder KJ and Senyei AE: Magnetic microspheres: A vehicle for selective targeting of drugs. Pharmac. Ther. 20:377, (1983).

    Article  CAS  Google Scholar 

  192. Widder KJ and Green R: Drug and Enzyme Targeting, Methods in Enzymology, 112, Academic Press, Inc. Press, Inc., Orlando, New York, (1985).

    Google Scholar 

  193. Winston D; Gale R; Meyer D and Young L: Infectious complications of human bone marrow transplantation. Medicine 58:1, (1979).

    Article  CAS  Google Scholar 

  194. Wojchowski DM and Sytkowski AJ: Detection of low-density cell-surface molecules using biotinylated fluorescent microspheres. Biochim. Biophysi. Acta. 857:61, (1986).

    Article  CAS  Google Scholar 

  195. Yalow RS and Benson SA: Immunoassay of endogenous plasma insulin in man. J. Clin. Invest. 39:1157, (1960).

    Article  CAS  Google Scholar 

  196. Yapel Jr, AF: Albumin Microspheres: Heat and Chemical Stabilization. In: Drug and Enzyme Targeting, Widder, KJ and Green R (eds): Meth. Enzymol. 112:3, (1985).

    Google Scholar 

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  1. Department of Immunology, M.D. Anderson Hospital and Tumor Institute, Houston, Texas, 77030, USA

    Chris D. Platsoucas

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    Mohamed S. El-Aasser

  2. S.C. Johnson & Son, Inc., Racine, WI, 53401, USA

    Robert M. Fitch

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Platsoucas, C.D. (1987). Biomedical Applications of Polymer Particles with Emphasis on Cell Separation. In: El-Aasser, M.S., Fitch, R.M. (eds) Future Directions in Polymer Colloids. NATO ASI Series, vol 138. Springer, Dordrecht. https://doi.org/10.1007/978-94-009-3685-0_21

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