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Glucosinolates in Rapeseeds: Analytical Aspects pp 109–124Cite as

Analysis of Individual Glucosinolates in Rapeseeds Comparison Between Different Methods

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Part of the book series: World Crops: Production, Utilization, Description ((WCPU,volume 13))

Abstract

The aim of our work is to contribute to the elaboration of a reliable quantitative method for individual glucosinolates determination in rapeseeds.

To emphasize the accuracy of the method, we have studied several important points:

  1. a)

    methanol - water (70%) extraction from seeds and seedcakes obtained after removal of the oil.

  2. b)

    column chromatographic purification of crude extracts

  • the influence of the height of D.E.A.E. A 25 columns (desulpho glucosinolates) and of Ecteolla Cellulose (intact glucosinolates) on the level of glucosinolates obtained is analysed

  • to avoid the use of pyridin, the pyridin - acetate buffer frequently used to prepare D.E.A.E. columns is changed by an acetic acid - sodium acetate buffer (pH : 5)

  1. c)

    HPLC analysis of desulphoglucosinolates and intact glucosinolates

  • comparison of desulphoglucosinolates and intact glucosinolates separations

  • choice of the detection wavelength

  • influence of column temperature

  1. d)

    Identification of desulphoglucosinolates from different cultivars of rapeseed (Garant, Ledos, Lirasol and Librador).

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References

  • Christensen, B., Kjaer, A., Madsen, Ø, Olsen, C., Olsen O. and Sørensen,H. 1982. Mass-spectrometric characteristics of some pertrimethyl-silylated desulphoglucosinolates. Tetrahedron, 38, 353–357.

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Author information

Authors and Affiliations

  1. Faculté des Sciences Agronomiques de l’Etat, B 5800, Gembloux, Belgium

    J-P. Wathelet (Chaire de Chimie Générale et Organique), M. Marlier (Chaire de Chimie Générale et Organique) & M. Séverin (Chaire de Chimie Générale et Organique)

  2. Station de Haute Belgique, Centre de Recherches Agronomiques de l’Etat, rue de Serpont, 48, B 6600, Libramont, Belgium

    R. Biston

Authors
  1. J-P. Wathelet
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  2. R. Biston
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  3. M. Marlier
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  4. M. Séverin
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Editor information

Editors and Affiliations

  1. B 5800, Gembloux, Belgium

    J-P. Wathelet (Faculté des Sciences Agronomiques de l’Etat, Chair de Chimie Générale et Organique) (Faculté des Sciences Agronomiques de l’Etat, Chair de Chimie Générale et Organique)

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© 1987 ECSC, EEC, EAEC, Brussels-Luxembourg

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Wathelet, JP., Biston, R., Marlier, M., Séverin, M. (1987). Analysis of Individual Glucosinolates in Rapeseeds Comparison Between Different Methods. In: Wathelet, JP. (eds) Glucosinolates in Rapeseeds: Analytical Aspects. World Crops: Production, Utilization, Description, vol 13. Springer, Dordrecht. https://doi.org/10.1007/978-94-009-3615-7_9

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  • DOI: https://doi.org/10.1007/978-94-009-3615-7_9

  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-94-010-8118-4

  • Online ISBN: 978-94-009-3615-7

  • eBook Packages: Springer Book Archive

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