Abstract
Total internal reflection fluorescence (TIRF) technique shows potential as the basis of a remote fluoroimmunoassay design (1,2). Evanescent excitation of fluorescently labelled antigens (Ag) complexed with surface immobilized antibodies (Ab), or vice versa, significantly simplifies the rinsing required in standard immunoassay techniques, and allows smaller sample volumes to be measured. The development of integrated waveguide optics as an evanescent spectroscopic technique has opened the possibility of optically detecting interfacially bound biological molecules in remote environments. This paper will review the research at the University of Utah on a remote fiber optic immunosensor and a polymer thin film evanescent sensor. We will also discuss the basic TIRF system as applied to the study of proteins at interfaces because it represents evanescently excited spectroscopy in its simplest form.
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© 1987 Martinus Nijhoff Publishers, Dordrecht
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Ives, J.T. et al. (1987). Total Internal Reflection Fluorescence Surface Sensors. In: Chester, A.N., Martellucci, S., Scheggi, A.M.V. (eds) Optical Fiber Sensors. NATO ASI Series, vol 132. Springer, Dordrecht. https://doi.org/10.1007/978-94-009-3611-9_24
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DOI: https://doi.org/10.1007/978-94-009-3611-9_24
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