Abstract
The amount of low molecular weight slimes (LMWS < 104 D) was usually higher than that of the high molecular weight slimes (HMWS > 104 D) produced by both Erwinia carotovora var. carotovora (Ecc) and E. carotovora var. atroseptica (Eca) 5 d after incubation at 25C. The concentration of the LMWS and HMWS differed according to the sugar added to the medium. In the case of Ecc, fructose was the most favorable for HMWS production while sucrose was the most favorable for LMWS production. However, in the case of Eca, sucrose was the most effective for the HMWS production, while fructose stimulated the production of LMWS. The percentage of protein in both slime types obtained from both bacteria differed according to the carbon source in the medium. Based on thin layer chromatographic separations of slime hydrolyzates, the slime from both Ecc and Eca cells contained common monomeric components namely glucose and rhamnose.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
Literature Cited
Bowles JA, Marsh DH. 1982. Glycocalyx, capsule, slime, or sheath--which is it? Letters, Vol. 48, No. 7. 295.
Dorn EM, Hellwij WR, Kanchmus HJ. 1974. Isolation and characterization of an 3. Chlorobenzoate degrading pseudomonad. Arch. Microbiol. 99:61–70.
Duguid JP. 1951. The demonstration of bacterial capsules and slime. J. Path. and Bacteriol. 63:673–685.
Eagon RG. 1956. Studies on polysaccharide formation by Pseudomonas fluorescens. Can. J. Microbiol. 2:673–676.
Haynes WC. 1951. Pseudomonas aeruginosa--its characterization and identification. J. Gen. Microbiol. 5:939–950.
Huang TS. 1980. Galactosyltransferase activity in Erwinia stewartii and its role in biosynthesis of extracellular polysaccharide. Physiol. Plant Pathol. 17:73–80.
Lowry OH, Rosebrough NJ, Fall AL, Randall RJ. 1951. Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193:265–275.
Van Alfen NK, McMillan BD, Drydon P. 1982. Characterization of the extracellular polysaccharide from Corynebacterium insidiosum. Phytopathology 72:945 (Abstr.).
Williams AG, Wimpenny JWT, Lawson CJ. 1973. The production of an extracellular polysaccharide by Pseudomonas-type microorganism. J. of Gen. Microbiol. 77:13.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1987 Martinus Nijhoff Publishers, Dordrecht
About this chapter
Cite this chapter
Abo-El-Dahab, M.K., El-Goorani, M.A., El-Kazazz, S.A. (1987). In Vitro Production of Extracellular Polysaccharides by Erwinia Carotovora Var. Carotovora and Erwinia Carotovora Var. Atroseptica . In: Civerolo, E.L., Collmer, A., Davis, R.E., Gillaspie, A.G. (eds) Plant Pathogenic Bacteria. Current Plant Science and Biotechnology in Agriculture, vol 4. Springer, Dordrecht. https://doi.org/10.1007/978-94-009-3555-6_36
Download citation
DOI: https://doi.org/10.1007/978-94-009-3555-6_36
Publisher Name: Springer, Dordrecht
Print ISBN: 978-94-010-8090-3
Online ISBN: 978-94-009-3555-6
eBook Packages: Springer Book Archive