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In Vitro Production of Extracellular Polysaccharides by Erwinia Carotovora Var. Carotovora and Erwinia Carotovora Var. Atroseptica

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Plant Pathogenic Bacteria

Abstract

The amount of low molecular weight slimes (LMWS < 104 D) was usually higher than that of the high molecular weight slimes (HMWS > 104 D) produced by both Erwinia carotovora var. carotovora (Ecc) and E. carotovora var. atroseptica (Eca) 5 d after incubation at 25C. The concentration of the LMWS and HMWS differed according to the sugar added to the medium. In the case of Ecc, fructose was the most favorable for HMWS production while sucrose was the most favorable for LMWS production. However, in the case of Eca, sucrose was the most effective for the HMWS production, while fructose stimulated the production of LMWS. The percentage of protein in both slime types obtained from both bacteria differed according to the carbon source in the medium. Based on thin layer chromatographic separations of slime hydrolyzates, the slime from both Ecc and Eca cells contained common monomeric components namely glucose and rhamnose.

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© 1987 Martinus Nijhoff Publishers, Dordrecht

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Abo-El-Dahab, M.K., El-Goorani, M.A., El-Kazazz, S.A. (1987). In Vitro Production of Extracellular Polysaccharides by Erwinia Carotovora Var. Carotovora and Erwinia Carotovora Var. Atroseptica . In: Civerolo, E.L., Collmer, A., Davis, R.E., Gillaspie, A.G. (eds) Plant Pathogenic Bacteria. Current Plant Science and Biotechnology in Agriculture, vol 4. Springer, Dordrecht. https://doi.org/10.1007/978-94-009-3555-6_36

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  • DOI: https://doi.org/10.1007/978-94-009-3555-6_36

  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-94-010-8090-3

  • Online ISBN: 978-94-009-3555-6

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