Abstract
Crown gall disease is caused by Agrobacterium tumefaciens and in grapes is manifested as tumors which most often occur on the trunks of vines. The size of the tumor reflects the cultivar’s sensitivity to the disease. We are using a tissue culture system to study the basis for varietal differences in sensitivity of grapes to crown gall disease. Stem explants from cultivars with various sensitivities to the disease were incubated on woody plant medium supplemented with equal concentrations of naphthaleneacetic acid and kinetin (0.5, 1.0, and 2.0 mg/l) at 28C for 16 d. As measured by increase in fresh weight, callus tissue growth from the explants was 3.0 to 6.5 times greater for highly sensitive variety than for insensitive varieties at all hormone concentrations (Fig. 1).
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© 1987 Martinus Nijhoff Publishers, Dordrecht
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Cleveland, G.L., Goodman, R.N. (1987). A Proposed Basis for Varietal Differences in Sensitivity of Grapes to Crown Gall Disease. In: Civerolo, E.L., Collmer, A., Davis, R.E., Gillaspie, A.G. (eds) Plant Pathogenic Bacteria. Current Plant Science and Biotechnology in Agriculture, vol 4. Springer, Dordrecht. https://doi.org/10.1007/978-94-009-3555-6_15
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DOI: https://doi.org/10.1007/978-94-009-3555-6_15
Publisher Name: Springer, Dordrecht
Print ISBN: 978-94-010-8090-3
Online ISBN: 978-94-009-3555-6
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