Abstract
Serological comparison of biotypes 1, 2, and 3 of Agrobacterium was made by double immunodiffusion (DID), indirect immunofluorescence (IIF), and the indirect enzyme-linked immunosorbent assay, (ELISA-I). Twenty four strains, representing of the three biotype and ten antisera obtained from sonicated whole cells, 0 antigens and glycoprotein extracts were used. The precipitin lines in DID, the titer of each antiserum in IIF and the serological relatedness quantified by ELISA, were studied.
Agrobacterium is a very heterogeneous genus despite the fact that all the strains studied were serologically related. In general, within one biotype the strains are not serologically homogeneous. Biotypes 1 and 2 are compact groups internally but different among each other, whereas biotype 3 is intermediate between biotypes 1 and 2. None of the antisera were useful for specifically distinguishing A. tumefaciens from A. radiobacter. The data obtained with the different techniques were compatible even being relevant to different aspects.
The results obtained show the difficulty in using the individual polyclonal antisera for serological detection of all of the strains of Agrobacterium tumefaciens.
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© 1987 Martinus Nijhoff Publishers, Dordrecht
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Alarcon, B., Lopez, M.M., Cambra, M., Gorris, M.T. (1987). Serological Study of Agrobacterium Biotypes. In: Civerolo, E.L., Collmer, A., Davis, R.E., Gillaspie, A.G. (eds) Plant Pathogenic Bacteria. Current Plant Science and Biotechnology in Agriculture, vol 4. Springer, Dordrecht. https://doi.org/10.1007/978-94-009-3555-6_11
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DOI: https://doi.org/10.1007/978-94-009-3555-6_11
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