Abstract
In the study of circadian rhythms many approaches are available to the chronobiologist. These range from the relatively simple measurement of gross locomotor activity (Pittendrigh & Daan, 1976) to the sophisticated assessment of neuronal activity from multiunit recordings (Borsook et al, 1984). Electrophysiological techniques have been used for a number of years in order to study neuronal activity. An alternative approach to the study of this process is to look at neurotransmitter release and metabolism. In this chapter we shall describe the use of two techniques, in vivo voltammetry and intracerebral dialysis, used routinely in our laboratory to study the release and metabolism of 5-hydroxytryptamine (5HT) in vivo. Both of these techniques can be used in freely moving animals and are therefore ideally suited to the requirements of the chronobiologist. The two techniques will be dealt with separately and then followed by some general remarks.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Adams RN & Marsden CA: Electrochemical detection methods for mono¬amine measurements in vitro and in vivo. In Handbook of Psycho- pharmacology (Eds LL Iversen, SD Iversen & SH Snyder), Vol 15, p 1–74. New York: Academic Press, 1982.
Borsook D, Moore-Ede MC, Hedberg T, Richardson G & Brennan MJW: Gamma-aminobutyric acid and the neural basis of circadian timekeeping: implications for pathophysiology and psychopharmacotherapy of circadian based disorders. Ann.Rev.Chronopharmac. 1, 53–56, 1984.
Borsook D, Moore-Ede MC, Hedberg T, Richardson G & Brennan MJW: Gamma-aminobutyric acid and the neural basis of circadian timekeeping: implications for pathophysiology and psychopharmacotherapy of circadian based disorders. Ann.Rev.Chronopharmac. 1, 53–56, 1984.
Cespuglio R, Faradji H, Hahn Z & Jouvet M: Voltammetric detection of brain 5-hudroxyindoleamines by means of electrochemically treated carbon fibre electrodes: Chronic recording for up to one month with movable cerebral electrodes in the sleeping or waking rat. In Measurement of Neurotransmitter Release in vivo (Ed CA Marsden). Chichester: John Wiley, p 173–191, 1984.
Cheng H-Y, Shenk J, Huff R & Adams PN. In vivo electrochemistry: behaviour of microelectrodes in brain tissue. J.Electroanalyt.Chem. 100, 23–31, 1979.
Gonon F, Buda M & Pujol J-F: Treated carbon fibre electrodes for measuring catechols and ascorbic acid. In Measurement of Neuro-transmitter Release in vivo (Ed CA Marsden). Chichester: John Wiley, p 153–171, 1984.
Marsden CA: Measurement of Neurotransmitter Release in vivo. Chichester: John Wiley, 1984.
Marsden CA, Brazell MP & Maidment NT: An introduction to in vivo electrochemistry. In Measurement of Neurotransmitter Release in vivo (Ed CA Marsden). Chichester: John Wiley, p 127–151, 1984.
Marsden CA & Routledge C: In vivo measurement of DOPAC, 5HIAA and 5HT in specific brain regions by intracerebral dialysis. Br.J.Pharmac. 82, 1984.
Marsden CA & Routledge C: In vivo measurement of DOPAC, 5HIAA and 5HT in specific brain regions by intracerebral dialysis. Br.J.Pharmac. 82, 1984.
Mefford IN, Oke AF & Adams RN: Regional distribution of ascorbate in human brain. Brain Res. 212, 223–226, 1981.
Pittendrigh LS & Daan S: A functional analysis of circadian pacemakers in nocturnal rodents. V. Pacemaker structure: A clock for all seasons. J.Comp.Physiol., 106, 333–335, 1976.
Sharp T, Maidment NT, Brazell MP, Zetterstrom T, Ungerstedt U, Bennett GW & Marsden CA: Changes in monoamine metabolites measured by simultaneous in vivo differential pulse voltammetry and intra¬cerebral dialysis. Neuroscience 12, 1213–1221, 1984.
Ungerstedt U: Measurement of neurotransmitter release by intracranial dialysis. In Measurement of Neurotransmitter Release in vivo (Ed CA Marsden). Chichester: John Wiley, p 81–105, 1984.
Zetterstrom T, Sharp T, Marsden CA & Ungerstedt U: In vivo measurement of dopamine and its metabolites by intracerebral dialysis: changes after d-amphetamine. J.Neurochem. 41, 1769–1773, 1983.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1987 Martinus Nijhoff Publisher
About this chapter
Cite this chapter
Martin, K.F., Marsden, C.A. (1987). Measurement of Brain Indoleamine Release in Vivo: Application to Chronobiology. In: Scheving, L.E., Halberg, F., Ehret, C.F. (eds) Chronobiotechnology and Chronobiological Engineering. NATO ASI Series, vol 120. Springer, Dordrecht. https://doi.org/10.1007/978-94-009-3547-1_38
Download citation
DOI: https://doi.org/10.1007/978-94-009-3547-1_38
Publisher Name: Springer, Dordrecht
Print ISBN: 978-94-010-8086-6
Online ISBN: 978-94-009-3547-1
eBook Packages: Springer Book Archive