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The Application of Histochemistry at the Light Microscopic Level to the Study of Nephrotoxicity

  • Chapter
Nephrotoxicity in the experimental and clinical situation

Part of the book series: Developments in Nephrology ((DINE,volume 19-20))

Abstract

Light microscopy continues to represent the major method by which nephropathies are identified in chemical safety assessment1 and in the clinical situation2. While ultrastructural studies provide a very important technique for detailed subcellular investigations, they generally do not contribute to diagnosis or treatment. Furthermore, these specialized methods are both time-consuming and costly. Histochemical techniques at the light microscopic level provide a broad approach to the study of renal injury that cannot, at present, be investigated as conveniently by electron microscopy or any biochemical method. Haematoxylin and eosin (H&E) is the routine histochemical stain used to vizualize cells, cellular structures and changes associated with tissue injury. Once cell changes have been identified by H&E a host of other histochemical techniques can be applied to help interpret the cause(s) of a lesion. There are, however, examples where subtle or specific cellular changes have not been identified by H&E, and hence more sophisticated techniques are needed. This full range of “routine” to highly “specialized” techniques can all be used to address the question of the molecular changes associated with a toxic insult.

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  1. P. H. Bach
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  2. N. J. Gregg
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  3. E. D. Wachsmuth
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Editors and Affiliations

  1. The Robens Institute, University of Surrey, Guildford, Surrey, England

    P. H. Bach

  2. Biochemical Toxicology Section, Imperial Chemical Industries plc, Alderley Park, Macclesfield, Cheshire, England

    E. A. Lock

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Bach, P.H., Gregg, N.J., Wachsmuth, E.D. (1987). The Application of Histochemistry at the Light Microscopic Level to the Study of Nephrotoxicity. In: Bach, P.H., Lock, E.A. (eds) Nephrotoxicity in the experimental and clinical situation. Developments in Nephrology, vol 19-20. Springer, Dordrecht. https://doi.org/10.1007/978-94-009-3367-5_2

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