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Fixation of Renal Tissue for Cytochemical Evaluation

  • Chapter
Nephrotoxicity in the experimental and clinical situation

Part of the book series: Developments in Nephrology ((DINE,volume 19-20))

Abstract

In many morphological studies of the kidney a major objective is conserving particular chemicals or groups of chemicals. This demands preservation of an activity (e.g. enzymic or antigenic) at or near its in vivo location and it implies conservation of the kidney architecture necessary to permit accurate localization. In this type of study the primary objectives are different from those pursued in morphometric work and the fixation (and embedding) methods differ markedly. Many of the preservation problems are not specific to the kidney and experience from other tissues may be directly applicable. Thus gaps can be filled to allow conclusions to be drawn that are relevant to the kidney.

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References

  1. Lewis, P.R. and Knight, D.P., Staining methods for sectional material, in Practical Methods in Electron Microscopy, Glauert, A.M., Ed., North Holland Press, Amsterdam, 1976, Ch. 5.

    Google Scholar 

  2. Horobin, R.W., Histochemistry, Gustav Fisher/Butterworth, Stuttgart, New York, London, 1982.

    Google Scholar 

  3. Janigan, D.T., The effects of aldehyde fixation on acid phosphatase activity in tissue blocks, J. Histochem. Cytochem., 13, 476, 1965.

    Article  PubMed  CAS  Google Scholar 

  4. Arborgh, B., Ericsson, J.L.E. and Helminen, H., Inhibition of renal acid phosphatase and aryl sulfatase activity by glutaraldehyde fixation, J. Histochem. Cytochem., 19, 449, 1971.

    Article  PubMed  CAS  Google Scholar 

  5. Hopwood, D., Fixatives and fixation: a review, Histochem. J., 1, 323, 1969.

    Article  PubMed  CAS  Google Scholar 

  6. Kraehenbuhl, J.P. and Jamieson, J.D., Localization of intracellular antigens using immunoelectron microscopy, in Electron Microscopy and Cytochemistry, Wisse, E., Ed., North Holland Press, Amsterdam, 1973.

    Google Scholar 

  7. Hed, J. and Enastrom, S., Detection of immune deposits in glomeruli: the masking effect on antigenicity of formalin in the presence of proteins, J. Immunol. Meth., 41, 57, 1981.

    Article  CAS  Google Scholar 

  8. Brantzaeg, P. and Rognum, T.O., Evaluation of tissue preparation methods and pair immuno fluorescence staining for immunocytochemistry of lymphomas, Histochem. J., 15, 655, 1983.

    Article  Google Scholar 

  9. Hanker, J.S., Kusyk, C.J., Bloum, F.E. and Pearse, A.G.E., The demonstration of dehydrogenases and monoamineoxidase by the formation of Osmium Blacks at the site of Hatchetts Brown, Histochemie, 33, 205, 1973.

    PubMed  CAS  Google Scholar 

  10. Prosperi, E. and Raap, A.K., Substrate protection during the fixation of ß-glucuronidase: cytochemical model system studies, Histochem. J., 14, 689, 1982.

    Article  PubMed  CAS  Google Scholar 

  11. Pearse, A.G.E., Histochemistry Theoretical and Applied, 3rd Ed., Churchill-Livingstone, Edinburgh, Ch. 2, 1972.

    Google Scholar 

  12. Christie, K.N. and Stoward, P.J., A quantitative study of the fixation of acid phosphatases by formaldehyde and its relevance to histochemistry, Proc. Roy. Soc. London B, 186, 137, 1974.

    Article  CAS  Google Scholar 

  13. Janigan, D.T., Tissue enzyme fixation studies. Is The effects of aldehyde fixation on ß glucuronidase, ß galactosidase, N–acetyl ß glucosamidase and ß glucosidase in tissue blocks, Lab. Invest., 13, 1038, 1964.

    Google Scholar 

  14. Wachsrauth, E.D., Assessment of immunocytochemical techniques with particular reference to the mixed-aggregation imminocytochemical technique. Trends in enzyme histochemistry and cytochemistry, Ciba Foundation Symposium, 73, Elsevier/North Holland, Amsterdam, 135, 1980.

    Google Scholar 

  15. Berod, A., Hartman, B.K. and Piyol, J.F., Importance of fixation in iinminohistochemistry: use of formaldehyde solutions at variable pH for the localization of tyrosine hydroxylase, J. Histochem. Cytochem., 29, 844, 1981.

    Article  PubMed  CAS  Google Scholar 

  16. Kyte, J., Imminoferritin determination of the distribution of (Na+K+) ATPase over the plasma membranes of renal convoluted tubules. I: Distal segment, J. Cell. Biol., 68, 287, Iis J. Cell. Biol., 68, 314, 1976.

    Google Scholar 

  17. Kumplainen, T., Human carbonic anhydrase EC 4.2.1.1 isoenzyme C effects of some fixatives on the antigenicity and improvement in the method of localization, Histochemistry, 72, 425, 1981.

    Article  Google Scholar 

  18. Vogt, A., Bockburn, H., Kosima, K. and Sonsaki, M., Electron microscopic localization of the nephron-toxic antibody in the glomeruli of the rat after intravenous application of purified nephrite-genic antibody-ferritin conjugates, J. Exp. Med., 127, 687, 1968.

    Article  Google Scholar 

  19. Graham, R.C. and Karnovsky, M.J., The early stages of absorption of injected horseradish peroxidase in the proximal tubules of the mouse kidney, J. Histochem. Cytochem., 14, 291, 1966.

    Article  PubMed  CAS  Google Scholar 

  20. Cope, G.H., Low temperature embedding in water miscible methacrylates after treatment with antifreezes, J. R. Microsc. Soc., 88, 235, 1968.

    PubMed  CAS  Google Scholar 

  21. Williams, M.A. and Lowrie, J.I., this volume.

    Google Scholar 

  22. Brahic, M., Haase, A.T. and Cash, E., Simultaneous in situ detection of viral RNA and antigens, Proc. Natl. Acad. Sci. USA, 81, 5445, 1984.

    Article  PubMed  CAS  Google Scholar 

  23. Myerson, D., Hackman, R.C., Nelson, J.A., Ward, D.C. and MacDougall, J.K., Widespread presence of histologically occult cytomegalovirins, Hum. Pathol., 15, 430, 1984.

    CAS  Google Scholar 

  24. Jamrich, M., Mahon, K.A., Garvis, E.R. and Gall, J.G., Histone RNA in amphibian oocytes visualized by in situ hybridisation to methacylate-embedded tissue sections, EMBO J., 3, 1939, 1984.

    PubMed  CAS  Google Scholar 

  25. Engfeit, B. and Hjertquist, S-O., The effect of various fixatives on the preservation of acid glycosaminoglycans in tissues, Acta Pathol. Microbiol. Scand., 71, 219, 1967.

    Google Scholar 

  26. Scott, J.E., Tigwell, M.J. and Sajdera, S.W., Loss of basophilic (sulphated) material from sections of cartilage treated with periodate solution, Histochem. J., 4, 155, 1972.

    Article  PubMed  CAS  Google Scholar 

  27. Benditt, E.P., Martin, G.M. and Plattner, H., Application of freeze-drying and formaldehyde vapour fixation to radioautographic localization of soluble aminoacids, in The Use of Radioautography in Studying Protein Synthesis, Leblond, C.P. and Warren, K., Eds., Academic Press, New York and London, 65, 1965.

    Google Scholar 

  28. Caro, L.G. and Palade, G.E., Protein synthesis and storage and discharge in the pancreatic exocrine cell. An autoradiographic study, J. Cell. Biol., 20, 473, 1964.

    Article  PubMed  CAS  Google Scholar 

  29. Clarke, S.L., The synthesis and storage of protein in isolated lymphoid cells, examined by autoradiography with the electron microscope, Am. J. Anat., 119, 375, 1966.

    Article  Google Scholar 

  30. Peters, T. and Ashley, C.A., An artifact in radioautography due to binding of the amino acids to tissues by fixatives, J. Cell. Biol., 33, 53, 1967.

    Article  PubMed  Google Scholar 

  31. Orkland, P.M. and Kravitz, E.A., Localization of the site of gamma-aminobutyric acid (GABA) uptake in lobster nerve-muscle preparations, J. Cell. Biol., 49, 75, 1971.

    Article  Google Scholar 

  32. Faeder, I.R. and Salpeter, M.M., Glutamate uptake by a stimulated insect nerve–muscle preparation, J. Cell. Biol., 46, 300, 1970.

    Article  PubMed  CAS  Google Scholar 

  33. Price, D.L., Stocks, J.W., Young, A. and Peck, K., Glycine-specific synapses in spinal cord. Identification by electron microscope autoradiography, J. Cell. Biol., 68, 389, 1976.

    Article  PubMed  CAS  Google Scholar 

  34. Lowrie, J.I., Baker, J.R.J, and Williams, M.A., in Renal Heterogenicity and Target Cell Toxicity, Bach, P.H., Ed., Wiley & Sons, New York, 1985

    Google Scholar 

  35. Wachsmuth, E.D. and Woodhaus, R., Uniform distribution and concentration of aminopeptidase in proximal tubules of pig kidney, J. Histochem. Cytochem., 21, 685, 1973.

    Article  PubMed  CAS  Google Scholar 

  36. Williams, M.A., Electron microscopic autoradiography: its application to protein biosynthesis, in Techniques in Protein Biosynthesis, Campbell, P.N. and Sargent, J.R., Eds., Academic Press, London, 125, Ch. 3, 1973.

    Google Scholar 

  37. Bogaroch, R., Studies on the intracellular localization of tritiated steroids, in Autoradiography of Diffusible Substances, Roth, L.J. and Stumpf, W.E., Eds., Academic Press, New York and London, 99, 1969.

    Google Scholar 

  38. Williams, M.A. and Baba, W.I., The localization of (H)-aldosterone and (3H)-Cortisol within renal tubular cells by electron microscopic autoradiography, J. Endocrinol., 39, 543, 1967.

    Article  PubMed  CAS  Google Scholar 

  39. Baker, J.R.J., this volume.

    Google Scholar 

  40. Baker, J.R.J., this volume.

    Google Scholar 

  41. Stirling, C.E. and Kinter, W.B., High resolution autoradiography of galactose-JH accumulation in rings of hamster intestine, J. Cell. Biol., 35, 585, 1967.

    Article  PubMed  CAS  Google Scholar 

  42. Rogers, A.W., Techniques of autoradiography, 3rd Ed., Elsevier, Amsterdam, 1979.

    Google Scholar 

  43. Williams, M.A., Autoradiography and immunocytochemistry, in Practical Methods in Electron Microscopy, Glauert, A.M., Ed., North Holland Press, Amsterdam, 6, 1977.

    Google Scholar 

  44. Williams, M.A., Autoradiography: its methodology at the present time, J. Microsc., 128, 79, 1982.

    Article  PubMed  CAS  Google Scholar 

  45. Cope, G.H. and Williams, M.A., Quantitative studies on neutral lipid preservation in electron microscopy, J. Roy. Microsc. Soc., 88, 259, 1968.

    CAS  Google Scholar 

  46. Cope, G.H. and Williams, M.A., Quantitative studies on the preservation of choline and ethanolamine phosphatides during tissue preservation for electron microscopy, I, J. Microsc., 90, 31, 1969.

    Article  PubMed  CAS  Google Scholar 

  47. Cope, G.H. and Williams, M.A., Quantitative studies on the preservation of choline and ethanolamine phosphatides during tissue preservation for electron microscopy, II, J. Microsc., 90, 47, 1969.

    Article  PubMed  CAS  Google Scholar 

  48. Williams, M.A., The assessment of electron microscopic autoradiographs, in Advances in Optical and Electron Microscopy, Barer, R. and Cosslett, E.V., Eds., Academic Press, London and New York, 219, Ch. 3, 1969.

    Google Scholar 

  49. Stein, O. and Stein, Y., Light and electron microscopic radioautography of lipids: techniques and biological applications, Adv. Lipid. Res., 9, 1, 1971.

    CAS  Google Scholar 

  50. Darrah, H.K., Hedley–Whyte, H. and Hedley-Whyte, E.T., Radioautography of cholesterol in lung, J. Cell. Biol., 49, 345, 1971.

    Google Scholar 

  51. Bayliss-High, O., Lipid histochemistry, Royal Microscopical Society, Oxford University Press, 69, 1984.

    Google Scholar 

  52. Seal1en, T.J. and Dietert, S.E., The quantitative retention of cholesterol in mouse liver prepared for electron microscopy by fixation in a digitonin containing aldehyde solution, J. Cell. Biol., 40, 802, 1969.

    Article  Google Scholar 

  53. Stuirpf, W.E. and Roth, L.J., Thin section cut at temperatures of -70 °C to -90 °C, Nature, 205, 712, 1965.

    Google Scholar 

  54. Barnard, T., Ultrastructural effects of the high molecular weight cryoprotectants Dextran and polyvinylpyrrolidone on liver and brown adipose tissue in vitro, J. Microsc., 120, 93, 1980.

    Article  PubMed  CAS  Google Scholar 

  55. Baker, J.R.J, and Appleton, T.C., A technique for electron microscopic autoradiography (and X–ray microanalysis) of diffusible substances using freeze-dried fresh frozen sections, J. Microsc., 108, 307, 1976.

    Article  CAS  Google Scholar 

  56. Appleton, T.C., Autoradiographs of soluble labelled compounds, J. Roy. Microsc. Soc., 83, 277, 1964.

    CAS  Google Scholar 

  57. Stumpf, W.E. and Roth, L.J., Vacuum freeze drying of frozen sections for dry mounting high resolution autoradiography, Stain Technol., 39, 219, 1964.

    PubMed  CAS  Google Scholar 

  58. Nagata, T. and Murata, F., Proc. 5th Int. Congr. Histochem. Cytochem., Bucharest, 1976.

    Google Scholar 

  59. Shaver, J. and Stirling, C.E., Ouabain binding to renal tubules of the rabbit, J. Cell. Biol., 76, 278, 1978.

    Article  PubMed  CAS  Google Scholar 

  60. Ernst, S.A. and Mills, J.W., Autoradiographic localization of ouabain-sensitive sodium pump sites in ion transporting epithelia, J. Histochem. Cytochem., 28, 72, 1980.

    Article  PubMed  CAS  Google Scholar 

  61. Fredericks, P.M., Autoradiography of diffusible substances, Thesis, Erasmus University, Rotterdam.

    Google Scholar 

  62. Harvey, D.M.R., Freeze-substitution. J. Microsc., 127, 209, 1982.

    Article  PubMed  CAS  Google Scholar 

  63. Simson, J.A.V., Blank, H.L. and Spicer, S.S., X-ray microanalysis of pyroantimonate precipitable cations, Scan. Electron Microsc., II, 779, 1979.

    Google Scholar 

  64. Baker, J.R.J., personal communication.

    Google Scholar 

  65. Kuhar, W.S. and Young, M.J., A new method of receptor autoradiography: [3H] opioid receptors in rat brain, Brain Res., 179, 255, 1979.

    Article  PubMed  Google Scholar 

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Authors
  1. M. A. Williams
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Editors and Affiliations

  1. The Robens Institute, University of Surrey, Guildford, Surrey, England

    P. H. Bach

  2. Biochemical Toxicology Section, Imperial Chemical Industries plc, Alderley Park, Macclesfield, Cheshire, England

    E. A. Lock

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© 1987 Martinus Nijhoff Publishers

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Williams, M.A. (1987). Fixation of Renal Tissue for Cytochemical Evaluation. In: Bach, P.H., Lock, E.A. (eds) Nephrotoxicity in the experimental and clinical situation. Developments in Nephrology, vol 19-20. Springer, Dordrecht. https://doi.org/10.1007/978-94-009-3367-5_1

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  • DOI: https://doi.org/10.1007/978-94-009-3367-5_1

  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-94-010-8012-5

  • Online ISBN: 978-94-009-3367-5

  • eBook Packages: Springer Book Archive

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