Abstract
Since the production of human monoclonal antibody has proved more difficult than the production of mouse monoclonal antibody, the obvious question must be asked: Is it worth the effort? The answer must be yes! Human monoclonal antibodies may obviate many of the difficulties that murine monoclonal antibodies used for therapy have encountered. The difficulties range from minor allergic reactions to anaphylaxis. Human monoclonal antibodies in this regard would also be safer and more useful in in vivo diagnostic and therapeutic applications. Moreover, murine monoclonal antibodies, being foreign proteins, are cleared by the reticuloendothelial system after several administrations due to their recognition by the human immune system. It should also be noted that murine monoclonal antibodies recognize a different range of antigens than do humans. The mouse immune system primarily recognizes xenogeneic antigens on human cells. A clinically relevant set of antigens on human tumor cell surfaces may generate a poor, if any, immune response in mice. Humans, on the other hand, may be better able to distinguish between normal and tumor cells.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Astaldi, G.C.B., Janssen, M.C., Lamsdorp, P., Willems, Ch., Zeijlemacher, W.P., and Oosterhof, F. Human endothelial culture supernatant (HECS): A growth factor for hybridomas. J. Immunol. 125:1411–1414, 1980.
Cote, R.J., Morrissey, D.M., Houghton, A.N., Beattie, Jr., E.J., Oettgen, H.F., and Old, L.J. Generation of human monoclonal antibodies reactive with cellular antigens. Proc Natl. Acad. Sci. USA, 80:2026–2030.
Gruenberg, J. and Sherman, W. Isolation and characterization of the plasma membrane of human erythrocytes infected with the malarial parasite Plasmodium falciparum. Proc. Natl. Acad. Sci. USA 80:1087–1091, 1983.
Hawkes, R., Niday, E., and Gordon, J. A dot-immunobinding assay for monoclonal and other antibodies. Anal. Biochem. 119: 142–147, 1982.
Houghton, A.N., Brooks, H., Cote, R.J., Taormina, M.C., Oettgen, H.F., and Old, L.J. Detection of cell surface and intracellular antigens by human monoclonal antibodies. J. Exp. Med. 158:53–65, 1983.
Imam, A., Taylor, C.R., and Tokes, Z.A. Use of human monoclonal antibodies for the detection of antigenic heterogeneity in the population of breast carcinoma cells. J. Histochem. Cytochem. 30:573 (abstract), 1982.
Imam, A., Drushella, M.M., and Tokes, Z.A. Generation and immunohistological characterization of human monoclonal antibodies to mammary carcinoma cells. Cancer Res. 45: 263–271, 1985.
Lo, M.M.S., Tsong, T.Y., Conrad, M.K., Srittmatter, S.M., Hester, L.D., and Snyder, S.H. Monoclonal antibody production by receptor-mediated electrically induced cell fusion. Nature 310:792–793, 1984.
Mai, S. and Chung, A.E. Cell attachment and spreading on extracelluar matrix-coated beads. Exp. Cell. Res. 152:500–509, 1984.
Oesterberg, L. and Pursch, E. Human x (mouse x human) hybridomas stably producing human antibodies. Hybridoma 2:361–367, 1983.
Oi, V.T. and Herzenberg, L.A. Immunoglobulin producing hybrid cell lines. In: Selected Methods in Cellular Immunology. W.H. Freeman, San Francisco, 1970, pp 351–372.
Olsson, L. and Kaplan, H.S. Human-human hybridomas producing monoclonal antibodies of predefined antigenic specificity. Proc. Natl. Acad. Sci. USA 77:5429–5431, 1980.
Schlom, J., Wunderlich, D., and Teramoto, Y.A. Generation of human monoclonal antibodies reactive with human mammary carcinoma cells. Proc. Natl.Acad. Sci. USA 77:6841–6845, 1980.
Sikora, K. and Wright, R. Human monoclonal antibodies to lung-cancer antigens. Br. J. Cancer 43:696–700, 1981.
Wunderlich, D., Teramoto, Y.A., and Schlom, J. The use of lymphocytes from axillary lymph nodes of mastectomy patients to generate human monoclonal antibodies. Eur. J. Cancer Clin. Oncol. 17: 719–730, 1981.
Zimmermann, U. Electric field-mediated fusion and related electrical phenomena. Biochim. Biophys. Acta 694:227–277, 1982.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1986 Martinus Nijhoff Publishers, Dordrecht
About this chapter
Cite this chapter
Waelti, E.R., Kraft, R., Cottier, H. (1986). Monoclonal Human Antitumor Antibodies: Search for Specific B-Lymphocytes Suitable for Fusion. In: Hollmann, K.H., Verley, J.M. (eds) New Frontiers in Mammary Pathology 1986. Developments in Oncology, vol 49. Springer, Dordrecht. https://doi.org/10.1007/978-94-009-3297-5_6
Download citation
DOI: https://doi.org/10.1007/978-94-009-3297-5_6
Publisher Name: Springer, Dordrecht
Print ISBN: 978-94-010-7980-8
Online ISBN: 978-94-009-3297-5
eBook Packages: Springer Book Archive