Murine Passive Cutaneous Anaphylaxis Test (PCA) for the “All or None” Determination of Allergenicity of Bovine Whey Proteins and Peptides
The study of allergenicity of proteins and peptides has attracted considerable research activities in the past two decades, utilizing several in vitro and in vivo techniques. The usefulness of the in vitro techniques, such as immuno-precipitation tests (1), radio-allergo-sorbent-test (2) and enzyme-linked — immuno-sorbent-test (3), is limited due to a poor correlation between results obtained by these tests and provocation tests in the patients. Consequently, the in vivo assays, passive cutaneous anaphylaxis test (PCA) (4, 5, 6,) and anaphylactic shock models (3) using guinea pigs in both cases, are still widely employed and recommended when assessing the allergenicity of various compounds.
KeywordsWhey Protein Evans Blue None None Concentrate Whey Protein Peptide Pool
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