Murine Passive Cutaneous Anaphylaxis Test (PCA) for the “All or None” Determination of Allergenicity of Bovine Whey Proteins and Peptides

  • O. M. Poulsen
  • J. Hau


The study of allergenicity of proteins and peptides has attracted considerable research activities in the past two decades, utilizing several in vitro and in vivo techniques. The usefulness of the in vitro techniques, such as immuno-precipitation tests (1), radio-allergo-sorbent-test (2) and enzyme-linked — immuno-sorbent-test (3), is limited due to a poor correlation between results obtained by these tests and provocation tests in the patients. Consequently, the in vivo assays, passive cutaneous anaphylaxis test (PCA) (4, 5, 6,) and anaphylactic shock models (3) using guinea pigs in both cases, are still widely employed and recommended when assessing the allergenicity of various compounds.


Whey Protein Evans Blue None None Concentrate Whey Protein Peptide Pool 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Lowenstein H, Krasilnikoff PA, Bjerrum OJ, Gudmund-Høyer E. Occurence of specific precipitins against bovine whey proteins in serum from children with gastrointerstinal disorders. Int Archs Allergy appl Immunol 1977;55:514–25.CrossRefGoogle Scholar
  2. 2.
    Basayau JP, Mallet E, Brunelle Ph, de Minibus C.H. Les intolerances au lait de vache. La Presse Medicale 1983;12:2041–43.Google Scholar
  3. 3.
    Zifshitz F. (ed). Clinical Disorders in Pediatric Nutrition. Vol. 4. Nutrition for Special Needs in Infancy. Protein Hydrolysates. Marcel Dekker, Inc. 1985.Google Scholar
  4. 4.
    Ovary Z. Passive Cutaneous Anaphylaxis.In: Ackroyd JF (ed) Immunological Methods. A Symposium. Oxford, Blackwell Scientific Publications 1964:259–83.Google Scholar
  5. 5.
    Lui CT, Das BR, Maurer PH. Immunochemical studies of the tryptic,chymotryptic and peptic peptides of heat denatured bovine serum albumin. Immunochemistry 1967;4:1–10.CrossRefGoogle Scholar
  6. 6.
    Takase M, Fukuwatari Y, Kawase K, Ogasa K, Suzuki S, Kuroume T. Antigenicity of Casein Enzymatic Hydrolysates. J. Dairy Sci 1979;62:1570–76.PubMedCrossRefGoogle Scholar
  7. 7.
    Poulsen OM, Hau J. Murine passive cutaneous anaphylaxis test (PCA) for the “all or none” determination of allergenicity of bovine whey proteins and peptides. Clicical Allergy 1987; 17:75–83.CrossRefGoogle Scholar
  8. 8.
    Jarrett EEE, Haig DM, McDougall W, McNulty E. Rat IgE production. II. Primary and booster reaginic antibody responses following intradermal or oral immunization. Immunology 1976; 30:671–77.PubMedGoogle Scholar
  9. 9.
    Otani H. Antigenicities of beta-lactoglobulin treated with proteolytic enzymes. Jpn J Zootech Sci 1981;52:47–52.Google Scholar
  10. 10.
    Levine BB, Vaz NM. Effect of combination of inbred strain, antigen, and antigen dose on immune responsiveness and reagin production in the mouse. A potential mouse model for immune aspects of human atopic allergy. Int Arch Allergy 1970;39:156–71.PubMedCrossRefGoogle Scholar
  11. 12.
    Swarbrick ET, Stokes CR, Scoothill JF. Absorbtion of antigens after oral immunization and the simultaneous induction of systemic tolerance. Gut 1978;39:121–25.Google Scholar
  12. 13.
    Andre C, Bazin H, Heremans JF. Influence of repeated administration of antigen by the oral route on specific antibodyproducing cells in the mouse spleen. Digestion 1973;9:166–75.PubMedCrossRefGoogle Scholar

Copyright information

© Martinus Nijhoff Publishers, Dordrecht 1988

Authors and Affiliations

  • O. M. Poulsen
    • 1
  • J. Hau
    • 2
  1. 1.Institute of Obstetrics and GynaecologyUniversity of OdenseOdenseDenmark
  2. 2.Department of Pathology, Laboratory Animal UnitRoyal Veterinary and Agricultural UniversityCopenhagenDenmark

Personalised recommendations