Abstract
The study of allergenicity of proteins and peptides has attracted considerable research activities in the past two decades, utilizing several in vitro and in vivo techniques. The usefulness of the in vitro techniques, such as immuno-precipitation tests (1), radio-allergo-sorbent-test (2) and enzyme-linked — immuno-sorbent-test (3), is limited due to a poor correlation between results obtained by these tests and provocation tests in the patients. Consequently, the in vivo assays, passive cutaneous anaphylaxis test (PCA) (4, 5, 6,) and anaphylactic shock models (3) using guinea pigs in both cases, are still widely employed and recommended when assessing the allergenicity of various compounds.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Lowenstein H, Krasilnikoff PA, Bjerrum OJ, Gudmund-Høyer E. Occurence of specific precipitins against bovine whey proteins in serum from children with gastrointerstinal disorders. Int Archs Allergy appl Immunol 1977;55:514–25.
Basayau JP, Mallet E, Brunelle Ph, de Minibus C.H. Les intolerances au lait de vache. La Presse Medicale 1983;12:2041–43.
Zifshitz F. (ed). Clinical Disorders in Pediatric Nutrition. Vol. 4. Nutrition for Special Needs in Infancy. Protein Hydrolysates. Marcel Dekker, Inc. 1985.
Ovary Z. Passive Cutaneous Anaphylaxis.In: Ackroyd JF (ed) Immunological Methods. A Symposium. Oxford, Blackwell Scientific Publications 1964:259–83.
Lui CT, Das BR, Maurer PH. Immunochemical studies of the tryptic,chymotryptic and peptic peptides of heat denatured bovine serum albumin. Immunochemistry 1967;4:1–10.
Takase M, Fukuwatari Y, Kawase K, Ogasa K, Suzuki S, Kuroume T. Antigenicity of Casein Enzymatic Hydrolysates. J. Dairy Sci 1979;62:1570–76.
Poulsen OM, Hau J. Murine passive cutaneous anaphylaxis test (PCA) for the “all or none” determination of allergenicity of bovine whey proteins and peptides. Clicical Allergy 1987; 17:75–83.
Jarrett EEE, Haig DM, McDougall W, McNulty E. Rat IgE production. II. Primary and booster reaginic antibody responses following intradermal or oral immunization. Immunology 1976; 30:671–77.
Otani H. Antigenicities of beta-lactoglobulin treated with proteolytic enzymes. Jpn J Zootech Sci 1981;52:47–52.
Levine BB, Vaz NM. Effect of combination of inbred strain, antigen, and antigen dose on immune responsiveness and reagin production in the mouse. A potential mouse model for immune aspects of human atopic allergy. Int Arch Allergy 1970;39:156–71.
Swarbrick ET, Stokes CR, Scoothill JF. Absorbtion of antigens after oral immunization and the simultaneous induction of systemic tolerance. Gut 1978;39:121–25.
Andre C, Bazin H, Heremans JF. Influence of repeated administration of antigen by the oral route on specific antibodyproducing cells in the mouse spleen. Digestion 1973;9:166–75.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1988 Martinus Nijhoff Publishers, Dordrecht
About this chapter
Cite this chapter
Poulsen, O.M., Hau, J. (1988). Murine Passive Cutaneous Anaphylaxis Test (PCA) for the “All or None” Determination of Allergenicity of Bovine Whey Proteins and Peptides. In: Beynen, A.C., Solleveld, H.A. (eds) New Developments in Biosciences: Their Implications for Laboratory Animal Science. Springer, Dordrecht. https://doi.org/10.1007/978-94-009-3281-4_17
Download citation
DOI: https://doi.org/10.1007/978-94-009-3281-4_17
Publisher Name: Springer, Dordrecht
Print ISBN: 978-94-010-7973-0
Online ISBN: 978-94-009-3281-4
eBook Packages: Springer Book Archive