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Murine Passive Cutaneous Anaphylaxis Test (PCA) for the “All or None” Determination of Allergenicity of Bovine Whey Proteins and Peptides

  • O. M. Poulsen
  • J. Hau

Abstract

The study of allergenicity of proteins and peptides has attracted considerable research activities in the past two decades, utilizing several in vitro and in vivo techniques. The usefulness of the in vitro techniques, such as immuno-precipitation tests (1), radio-allergo-sorbent-test (2) and enzyme-linked — immuno-sorbent-test (3), is limited due to a poor correlation between results obtained by these tests and provocation tests in the patients. Consequently, the in vivo assays, passive cutaneous anaphylaxis test (PCA) (4, 5, 6,) and anaphylactic shock models (3) using guinea pigs in both cases, are still widely employed and recommended when assessing the allergenicity of various compounds.

Keywords

Whey Protein Evans Blue None None Concentrate Whey Protein Peptide Pool 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Martinus Nijhoff Publishers, Dordrecht 1988

Authors and Affiliations

  • O. M. Poulsen
    • 1
  • J. Hau
    • 2
  1. 1.Institute of Obstetrics and GynaecologyUniversity of OdenseOdenseDenmark
  2. 2.Department of Pathology, Laboratory Animal UnitRoyal Veterinary and Agricultural UniversityCopenhagenDenmark

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