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Analytical Determination of Orthophosphate in Water

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Phosphorus in Freshwater Ecosystems

Part of the book series: Developments in Hydrobiology ((DIHY,volume 48))

Abstract

Methods for orthophosphate determination and the problems of interferences are reviewed.

An important group of methods utilizes the phosphomolybdate complex. The complexation step, the reduction step and the extraction step are treated separately and alternative procedures compared.

Another group of methods uses ion association complexes; they are primarily used in physiology and not commonly used in water analyses today.

Enzymatic methods for orthophosphate analysis in natural waters have been developed lately and are ready for application in selected waterbodies.

Flame spectroscopic, fluorometric, gas chromatographic, ion exclusion chromatographic, inductively coupled plasma and other methods are also shortly presented.

Radiobiological bioassays for orthophosphate are also available.

In conclusion it was emphasized that the most common and reliable technique still is the molybdenum blue method as modified by Murphy & Riley (1962).

The need for more specific and sensitive methods is particularly strong in investigations of phosphorus turnover and phosphorus limitation in natural waters. For these purposes the enzymatic phosphatase methods has advantages due to their specificity for orthophosphate and they might offer an alternative to the molybdenum blue method.

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References

  • Ahlgren, G., 1970. Test av analysmetoder för fosfatfosfor. Inst, of Limnology Univ. Uppsala, 17 pp., (in Swedish).

    Google Scholar 

  • Akamatzu, S., 1952. Some micromethods for enzyme studies. J. Biochem. (Tokyo) 39: 203–210.

    Google Scholar 

  • Allen, H. E. &R. B. Hahn, 1969. Determination of phosphate in natural waters by activation analysis of tungsto-phosphoric acid. Envir. Sci. Technol. 3: 844–848.

    Article  CAS  Google Scholar 

  • Amer. Public Health Assn., 1965. Standard methods for examination of water and wastewater including bottom sediments and sludges (12 th ed.), Amer. Public Health Association, NY, 769 pp.

    Google Scholar 

  • Ammon, R. &K. Hinsberg, 1936. Colorimetrishe Phosphor-und Arsensäurebestimmung mit Ascorbinsäure. Hoppe-Seyler’s Z.239: 207–216.

    Article  CAS  Google Scholar 

  • Atkins, W. R. G., 1923. The phosphate content of fresh and salt water in its relationship to the growth of the algal plankton. J. mar. biol. Ass. UK 13: 119–150.

    Article  CAS  Google Scholar 

  • Bedrosian, A. J., R. K. Skogerboe &G. H. Morrison, 1968. Direct emission spectrographic methods for trace elements in biological materials. Analyt. Chem. 40: 854–860.

    Article  CAS  Google Scholar 

  • Boltz, D. F. &C. H. Lueck, 1958. Phosphorus. In D. F. Boltz (ed.), Colorimetric determination of nonmetals. Inter-science, NY Chemical Analysis, 8: 29–46.

    Google Scholar 

  • Bowen, H. J. M., 1967. Comparative elemental analyses of standard plant material. Analyst 92: 124–131.

    Article  CAS  Google Scholar 

  • Burchfìeld, H. P., D. E. Johnson, J. W. Rhoades &R. J. Wheeler, 1965. Selective detection of phosphorus, sulfur and halogen compounds in the gas chromatograpy of drugs and pesticides. J. Gaschromatogr. 3: 28–34.

    Google Scholar 

  • Burton, J. D. &J. P. Riley, 1956. Determination of soluble phosphate and total phosphorus in sea-water and total phosphorus in marine muds. Mikrochim. Acta: 1350–1365.

    Google Scholar 

  • Burton, J. D., 1973. Problems in the analysis of phosphorus compounds Wat. Res. 7: 291–307.

    Article  Google Scholar 

  • Campbell, F. R. &R. F. Thomas, 1970. Automated method for determining and removing silica interference in determination of soluble phosphorus in lake and stream waters. Envir. Sci. Technol. 4: 602–604.

    Article  CAS  Google Scholar 

  • Chamberlain, W. M. &J. Shapiro, 1969. On the biological significance of phosphate analysis; comparison of stan-dard an new methods with a bioassay. Limnol. Oceanogr. 14: 921–927.

    Article  CAS  Google Scholar 

  • Chamberlain, W. M. &J. Shapiro, 1973. Phosphate measurements in natural waters -A critique. In E. J. Griffith, A. M. Beeton, J. M. Spencer &D. I. Mitchell (eds.), Environmental Phosphorus Handbook, J. Wiley &Sons, NY: 355-366.

    Google Scholar 

  • Chaube, A. &V. K. Gupta, 1983. Spectrophotometric determination of phosphate in polluted waters by solvent extraction of molybdenum blue. Analyst 108: 1141–1144.

    Article  CAS  Google Scholar 

  • Crouch, S. R. &H. V. Malmstadt, 1967. A mechanistic investigation of molybdenum blue method for determination of phosphate Analyt. Chem. 39: 1084–1089.

    Article  CAS  Google Scholar 

  • Czensny, R., 1932. Zweckmässige Ausgestaltung der quantitativen Wasseranalyse zur Beurteilung fischereilicher Belange. Die Bestimmungen des pH-Wertes, des Eisens, des Ammoniaks, der Nitrite, der Kiselsäure, der Phosphorsäure und der Sulfate im Wasser. Z. Fisch. 30: 647–689.

    Google Scholar 

  • Czensny, R., 1960. Wasser-, Abwasser-und Fischereichemie. VEB Verlag Technik, Berlin, 429 pp.

    Google Scholar 

  • Denigés, G., 1920. Reaction de coloration extremement sensible des phosphates et des arseniates. Ses applications. C.r. Acad. Sci. Paris, 171: 802–804.

    Google Scholar 

  • Dick, W. A. &M. A. Tabatabai, 1977. Determination of orthophosphate in aqueous solutions containing labile organic and inorganic phosphorus compounds. J. envir. Qual. 6: 82–85.

    Article  CAS  Google Scholar 

  • Downes, M. T., 1978. Automated determination of low reactive phosphorus concentrations in natural waters in presence of arsenic, silicon and mercuric chloride. Wat. Res. 12: 743–745.

    Article  CAS  Google Scholar 

  • Edwards, G. P., A. H. Molof &R. Schneeman, 1965. Determination of orthophosphate in fresh and saline waters. J. am. Wat. Wks. Ass. 57: 917–925.

    CAS  Google Scholar 

  • Fiske, C. H. &Y. Subbarow, 1925. The colorimetric determination of phosphorus. J. Biol. Chem. 66: 375–400.

    CAS  Google Scholar 

  • Furman, N. H. (ed.), 1962. Scott’s standard methods of chemical analysis, 6th ed. vol. I, van Nostrand, Princeton, NJ, 814 pp.

    Google Scholar 

  • Garen, A. &O. Levinthal, 1960. A fine-structure genetic and chemical study of the enzyme alkaline phosphatase of E. coli. Biochim. Biophys. Acta 38: 470–483.

    Article  PubMed  CAS  Google Scholar 

  • Golterman, H. L., 1960. Studies on the cycle of elements in fresh water Acta Bot. Neerlandica 9: 1–58.

    CAS  Google Scholar 

  • Golterman, H. L. &I. M. Wurtz, 1961. A sensitive rapid determination of inorganic phosphate in presence of labile phosphate esters Analyt. chim. Acta 25: 295–297.

    CAS  Google Scholar 

  • Greenfield, L. J. &F. A. Kalber, 1955. Inorganic phosphate measurement in sea water. Bull. mar. Sci. Gulf Caribb. 4: 323–335.

    Google Scholar 

  • Griffith, E. J., A. M. Beeton, J. M. Spencer &D. I. Mitchell, 1973. (eds.) Environmental phosphorus handbook. J. Wiley &Sons, NY, 718 pp.

    Google Scholar 

  • Guyon, J. G. &W. D. Shults, 1969. Rapid phosphate determination by fluorimetry. J. am. Wat. Wks Ass. 61: 403–404.

    CAS  Google Scholar 

  • Hahn, R. B. &T. M. Schmitt, 1969. Determination of phosphate in water with tungsten-185. Analyt. Chem. 41: 359–360.

    Article  CAS  Google Scholar 

  • Harwood, J. E. &W. H. J. Hattingh, 1973. Colorimetric methods of analysis of phosphorus at low concentrations in water. In E. J. Griffith, A. M. Beeton, J. M. Spencer &D. I. Mitchell (eds), Environmental Phosphorus Handbook, J. Wiley &Sons, NY: 289–302.

    Google Scholar 

  • Harwood, J. E., R. A. van Steenderen &A. L. Kuhn, 1969. A comparison of some methods for total phosphate analysis. Wat. Res. 3: 425–432.

    Article  CAS  Google Scholar 

  • Holzbecher, I. &D. E. Ryan, 1973. The fluorimetric determination of phosphate with thiamine. Analyt. chim. Acta 64: 147–150.

    Article  CAS  Google Scholar 

  • Itaya, K. &M. Ui, 1966. A new micromethod for the colorimetric determination of inorganic phosphate. Clin. chim. Acta 14: 361–366.

    Article  PubMed  CAS  Google Scholar 

  • Jackson, M. L., 1958. Soil chemical analysis. Prentice Hall, NY, 498 pp.

    Google Scholar 

  • Janse, T. A. H. M., P. F. A. van Der Wiel &G. Kateman, 1983. Experimental optimization procedures in the determination of phosphate by flow-injection analysis. Analyt. chim. Acta: 89–102.

    Google Scholar 

  • Jean, M., 1955. Sur la formation el la reduction de l’acide phosphomolybdique et de l’acide arsen omolybdique. C.r. Acad. Sci. Paris, 240: 22–37.

    Google Scholar 

  • Jones, P. G. W. &C. P. Spencer, 1963. Comparison of several methods of determining inorganic phosphate in sea water. J. mar. biol. Ass. UK 43: 251–273.

    Article  CAS  Google Scholar 

  • Juday, C., E. A. Birge, G. I. Kemmerer &R. J. Robinson, 1928. Phosphorus content of lake waters of northeastern Wisconsin. Trans. Wis. Acad. Sci. 23: 233–248.

    Google Scholar 

  • Kaelble, E. F. (ed.), 1967. Handbook of x-rays, McGraw-Hill, NY, 339 pp.

    Google Scholar 

  • Kaelble, E. F., 1973. Spectrometric methods for determining phosphorus and its compounds. In E. J. Griffith, A. M. Beeton, J. M. Spencer &D. I. Mitchell (eds), Environmental Phosphorus Handbook, J. Wiley &Sons, NY: 341–350.

    Google Scholar 

  • Kallner, A., 1975. Determination of phosphate in serum and urine by a single step Malachite-Green method. Clin. chim. Acta. 59: 35–39.

    Article  PubMed  CAS  Google Scholar 

  • Karmen, A., 1969. Differential specificity in detecting phosphorus, nitrogen, and halogens with alkali flames. J. chro-matogr. Sci. 7: 541–549.

    CAS  Google Scholar 

  • King, E. J., 1932. The colorimetric determination of phosphorus. Biochem. J. 26: 292–297.

    PubMed  CAS  Google Scholar 

  • Kirkbright, G. F., R. Narayanaswamy &T. S. West, 1971. Spectrofluorimetric determination of orthophosphate as Rodamine B molybdophosphate. Analyt. Chem. 43: 1434–1438.

    Article  CAS  Google Scholar 

  • Kirkbright, G. F., R. Narayanaswamy &T. S. West, 1972. Spectrofluorimetric determination of orthophosphate as quininemolybdophophate. Analyst 97: 174–181.

    Article  CAS  Google Scholar 

  • Kuenzeler, E. J. &B. H. Ketchum, 1962. Rate of phosphorus uptake by Pheodactylum tricornutum. Biol. Bull. 123: 134–145.

    Article  Google Scholar 

  • Levine, H., J. J. Rowe &F. S. Grimaldi, 1955. Molybdenum blue reaction and determination of phosphorus in waters containing arsenic silicon, and germanium. Analyt. Chem. 27: 258–262.

    Article  CAS  Google Scholar 

  • Lei, W., K. Fujiwara &K. Fuwa, 1983. Determination of phosphorus in natural waters by Long-capillary-cell adsorption spectrometry. Analyt. Chem. 55: 951–955.

    Article  Google Scholar 

  • Martin, J. B. &D. M. Doty, 1949. Determination of inorganic phosphate Analyt. Chem. 21: 965–967.

    CAS  Google Scholar 

  • Matthews, D. R., W. D. Shults, M. R. Guerin &J. A. Dean, 1971. Extraction -Derivatization -Gaschromatograpic determination of trace phosphate in aqueous media. Analyt. Chem. 43: 1582–1585.

    Article  CAS  Google Scholar 

  • McCune, H. W. &G. J. Arquette, 1955. Precipitation of pyrophosphate and triphosphate with tris (ethylene-diamine) cobalt (III)chloride and hexaminecobalt(III)-chloride. Analyt. Chem. 27;; 401–405.

    Article  CAS  Google Scholar 

  • Motomizu, S., T. Wakimoto &K. Toei, 1984. a. Solvent extraction -spectrophotometric determination of phosphate with molybdate and Malachite Green in river and sea-water. Talanta, 31: 235–240.

    Article  PubMed  CAS  Google Scholar 

  • Motomizu, S., H. Mikasa, M. Oshima &K. Toei, 1984. b. Continuous flow method for the determination of phosphorus using the fluorescence quenching or Rhodamine 6 G with molybdophosphate. Buneski Kagaku 33: 116–118.

    Article  CAS  Google Scholar 

  • Murphy, J. &J. P. Riley, 1958. A single-solution method for the determination of soluble phosphate in sea-water. J. mar. biol. Ass. UK 37:235–240.

    Article  Google Scholar 

  • Murphy, J. &J. P. Riley, 1962. A modified single-solution method for the determination of phosphate in natural waters. Analyt. chim. Acta 27: 31–36.

    Article  CAS  Google Scholar 

  • Namiki, H., 1961. Spectrophotometric determination of phosphate in sewage by extraction of molybdenum blue. Bunseki Kagaku 10: 945–951.

    Article  CAS  Google Scholar 

  • Ohle, W., 1938. Zur Vervollkommnung der hydrochemishen Analyse III. Die Phosphorbestimmung. Z. angew. Chem. 51:906–911.

    Article  CAS  Google Scholar 

  • Olsen, S., 1967. Recent trends in the determination of orthophosphate in water. In H. L. Golterman &R. S. N. V. Clymo (eds), Chemical Environment in the Aquatic Habitat. Noord-Hollandsche uitgevers Maats-chappij, Amsterdam: 63–105.

    Google Scholar 

  • Osmond, F., 1887. Sur une reaction pouvant sevir en dosage colorimetrique du phosphore dans les fontes, les aciers. Bull. Soc. chim., Paris. 47: 745–748.

    Google Scholar 

  • Pettersson, K., 1979. Enzymatic determination of ortho-phosphate in natural waters. Int. Revue ges. Hydrobiol. 64: 585–607.

    CAS  Google Scholar 

  • Pierce, W. C, E. L. Hoenish &D. T. Sawyer, 1959. Quantitative analysis, 4th. ed., J. Wiley &Sons. NY: 369–373.

    Google Scholar 

  • Proctor, C. M. &D. W. Hood, 1954. Determination of inorganic phosphate in sea water by an iso-butanol extraction procedure. J. mar. Res. 13: 122–132.

    CAS  Google Scholar 

  • Rigler, F. H., 1966. Radiobiological analysis of inorganic phosphorus in lake water. Verh. int. Ver. Limnol. 16: 465–470.

    Google Scholar 

  • Shapiro, J., 1971. Arsenic and phosphate: Measured by various techniques. Science, 171: 234.

    Article  PubMed  CAS  Google Scholar 

  • SIS, 1984. Vattenundersökningar bestämmning av fosfat (SIS.028126). SIS, Stockholm, 8 pp. (in Swedish).

    Google Scholar 

  • Stainton, M. P., 1980. Errors in the molybdenum blue methods for determining ortho-phosphate in fresh water. Can. J. Fish aquat. Sci. 37: 472–478.

    Article  CAS  Google Scholar 

  • Stephens, K., 1963. Determination of low phosphate concentrations in lake and marine waters. Limnol. Oceanogr. 8: 361–362.

    Article  Google Scholar 

  • Stevens, R. J., 1979. Evaluation of an enzymatic method for orthophosphate determination in freshwaters. Wat. Res. 13: 763–770.

    Article  CAS  Google Scholar 

  • Strickland, J. D. H. &T. R. Parsons, 1965. A manual of sea water analysis. Bull. Fish Res. Bd Can. No. 125. 185 pp.

    Google Scholar 

  • Strickland, J. D. H. &T. R. Parsons, 1968. A practical handbook of seawater analysis. Bull. Fish Res. Bd Can. No. 167. 185 pp.

    Google Scholar 

  • Strickland, J. D. H. &L. Solorzano, 1966. Determination of monoesterase hydrolysable phosphate and phosphomonoesterase activity in sea water. In H. Barnes (ed.), Some contemporary studies in Marine Science. Allen &Unwin, Lond. 665–675.

    Google Scholar 

  • Sugawara, K. &S. Kanamori, 1961. Spectrophotometric determination of submicrogram quantities of orthophosphate in natural waters. Bull. chem. Soc. Japan, 34: 258–261.

    Article  CAS  Google Scholar 

  • Taft, J. L., M. E. Loftus &W. R. Taylor, 1977. Phosphate uptake from phosphomonoesters by phytoplankton in the Chesapeake Bay. Limnol. Oceanogr. 22: 1013–1021.

    Article  Google Scholar 

  • Tanaka, K. &T. Ishizuka, 1982. Determination of phosphate in wastewaters by Ion-exclusion-chromatography with flow colometric detection. Wat. Res. 16: 719–723.

    Article  CAS  Google Scholar 

  • Tyree, Jr., S. Y. &M. A. O. Bynum, 1984. A method for nitrate and phosphate in saline water. Limnol. Oceanogr. 29: 1337–1339.

    Article  CAS  Google Scholar 

  • Wagner, W., C. J. Hull &G. E. Markle, 1956. Advanced Analytical Chemistry, Reinhold, NY, 282 pp.

    Google Scholar 

  • Wayman, C. H., 1964. Simultaneous determination of sulphur and phosphorus in water by neutron activation analysis. Analyt. Chem. 36: 665–666.

    Article  CAS  Google Scholar 

  • van Wazer, J. R., 1958. Phosphorus and its compounds. I Chemistry., II: Technology, biological functions, and applications. Interscience, NY, 2046 pp.

    Google Scholar 

  • Whatly, T. P. &L. W. Ferrara, 1973. Gravimetric Analysis of phosphorus compounds. In E. J. Griffith, A. M. Beeton, J. M. Spencer &D. I. Mitchell (eds.), Environmental Phosphorus Handbook, J. Wiley &Sons, NY: 313–326.

    Google Scholar 

  • Wilson, H. N., 1954. The determination of phosphate in the presence of soluble silicates. Analyst 79: 535–546.

    Article  CAS  Google Scholar 

  • Vogler, P., 1965. Beiträge zur Phosphatanalytik in der Limnologie II. Die Bestimmung des gelösten Orthophos-phates. Fortschr. Wasserchem. 109–119.

    Google Scholar 

  • Won, C. H., 1964. Sensitive spectrophotometric determination of orthophosphate in natural waters by a modified molybdenum (V) thiocyanate method. Nippon Kagaku Zasshi 85: 859–863.

    Article  Google Scholar 

  • Zaugg, W. S., 1967. Determination of phosphate in fresh and seawater by atomic absorption spectrophotometry Atomic Absorpt. Newslett. 6: 63–65.

    CAS  Google Scholar 

  • Zaugg, W. S. &R. J. Knox, 1966. Indirect determination of iorganic phosphate by atomic absorption spectrophotometric determination of molybdenum. Analyt. Chem. 38: 1759–1760.

    Article  CAS  Google Scholar 

  • Zimmerman, P., 1961. Chemische und bakteriologische Untersuchung im unteren Zurichsee während der Jahre 1948-1957. Schweiz. Z. Hydrol. 23: 342–397.

    Article  Google Scholar 

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  1. Department of Limnology, Uppsala University, Box 557, S-751 22, Uppsala, Sweden

    Ola Broberg & Kurt Pettersson

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  1. Ola Broberg
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  2. Kurt Pettersson
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Gunnar Persson Mats Jansson

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© 1988 Kluwer Academic Publishers

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Broberg, O., Pettersson, K. (1988). Analytical Determination of Orthophosphate in Water. In: Persson, G., Jansson, M. (eds) Phosphorus in Freshwater Ecosystems. Developments in Hydrobiology, vol 48. Springer, Dordrecht. https://doi.org/10.1007/978-94-009-3109-1_5

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  • DOI: https://doi.org/10.1007/978-94-009-3109-1_5

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  • Print ISBN: 978-94-010-7898-6

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