Summary
The application of bacterial mutagenicity tests to drinking water has shown the presence of mutagens. Invariably, mutagens generated during water treatment chlorination account for much of the mutagenicity detected in most surface-water-derived drinking waters. These mutagens are formed from widespread, naturally occurring precursors, such as humic substances, although other substances, especially amino acids may be involved. Most of the chlorination-derived mutagens identified account for little of the mutagenicity of drinking water. However, one mutagen, MX, is highly potent and may have a significant contribution. Its occurrence and toxicity need full evaluation. Other oxidants/disinfectants, such as ozone and chlorine dioxide, can also generate mutagens but these are likely to differ from those produced by chlorination. Granular activated carbon is effective in removing chlorination-derived mutagens but less effective for precursors. Dechlorinating agents, such as sulphur dioxide, can eliminate some of the mutagens produced by chlorination. The bacterial mutagenicity test cannot give information on the actual risks to health posed by the mutagens. Such information could arise from follow up work on identified major mutagens and from application of other bioassays more indicative of effects in man. Consequently, at the present time it is difficult to justify major changes to treatment practice aimed solely at controlling mutagenicity.
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References
AMES, B.N. (1971). The detection of mutagens with enteric bacteria. A. Hollander (ed), Chemical Mutagens, Principles and Methods of Detection, Vol.1, Plenum Press, 267–282.
FORSTER R. et al. (1983). Use of the fluctuation test to detect mutagenic activity in unconcentrated samples of drinking water in the U.K. R. L. Jolley et al. (eds.), Water Chlorination, Vol. 4, Ann Arbor Scientific Publ. 1189–1197.
WILCOX P. et al. (1986). Isolation and chacterisation of mutagens from drinking water. A. Leonard and M. Kirsch-Volders (eds.), Proceedings of the XVIth Annual Meeting, European Environmental Mutagen Society, 92–103.
FAWELL, J.K. et al. (1986). Health aspects of organics in drinking water. Technical Report TR 231, Water Research Centre, Marlow, Bucks., U.K.
Van der GAAG, M.A. et al. (1985). The influence of water treatment processes on the presence of organic surrogates and mutagenic compounds in water. Sci. Total Environ. 47, 137–153.
KOOL, H.J. and Van KREIJL, C.F. (1984). Formation and removal of mutagenic activity during drinking water preparation. Water Res. 18, 1001–1016.
LOPER, J.C. et al. (1985). Continuous removal of both mutagens and mutagen-forming potential by an experimental full-scale granular activated carbon-treatment system. Environ. Sci. Technol. 19, 333–339.
Van HOOF, F. et al. (1985). Formation of mutagenic activity during surface water pre-ozonation and its removal in drinking water treatment. Chemosphere 14, 501–509.
BAKER, A. and WILCOX, P. (1987). An investigation on the origin of mutagens in water samples collected from three different sites along a lowland river. Report PRD 1468-M, Water Research Centre, Marlow, Bucks., U.K.
ROOK, J.J. (1974). Formation of haloforms during chlorination of natural waters. Water Treat. Exam. 23, 234–243.
FIELDING, M. et al. (1981). Organic micro-pollutants in drinking water. Technical Report TR 159, Water Research Centre, Marlow, Bucks., U.K.
CHRISTMAN, R.F. et al. (1984). Oxidative degradation of aquatic humic material. Second International Humic Substances Conference, Birmingham.
RECKHOW, D.A. and SINGER, P.C. (1985). Mechanisms of organic halide formation during fulvic acid chlorination and implications with respect to pre-ozonation. R.L. Jolley et al. (eds.), Water Chlorination, Vol.5, Lewis Publ. 1229–1257.
De LEER, E.W.B. et al. (1985). Identification of intermediates leading to chloroform and C-4 diacids in the chlorination of humic acid. Environ. Sci. Technol. 19, 512–522.
FIELDING, M. and HORTH, H. (1986). Formation of mutagens and chemicals during water treatment chlorination. Wat. Supply, 4, 103–126.
De LEER, E.W.B. et al. (1986). Chlorination of ω-cyanoalkanoic acids in aqueous medium. Environ. Sci. Technol. 20, 1218–1223.
HOLMBOM, B. et al. (1981). Fractionation, isolation and identification of an Ames-mutagenic compound in Kraft chlorination effluents. Tappi 64 (3), 172–174.
MEIER, J.R. et al. (1986). Mutagenic by-products from chlorination of humic acid. Environ. Health Perspect. 69, 101–107.
TABOR, M.W. and LOPER, J.C. (1980). Separation of mutagens from drinking water using coupled bioassay/analytical fractionation. Intern. J. Environ. Anal. Chem. 8, 197–215.
HORTH, H. et al (1987). Techniques for the fractionation and identification of mutagens produced by water treatment chlorination. I.H. Suffet and M. Malaiyandi (eds.). Organic Polutants in Water, Advances in Chemistry Series 214. American Chemical Society.
HORTH, H. et al. (1987). The production of organic chemicals and mutagens during chlorination of amino acids in water. 6th Conference on Water Chlorination, Oak Ridge, Tennessee.
ERTEL, J.R. et al. (1986). Dissolved humic substances of the Amazon river system. Limnol. Oceanogr. 31, 739–754.
MARUOKA, S. (1986). Analysis of mutagenic by-products produced by chlorination of humic substances by thin layer chromatography and high-performance liquid chromatography. Sci. Total Environ. 54, 195–205.
HEMMING, J. et al. (1986). Determination of the strong mutagen 3-chloro-4(dichloro-methyl)-5-hydroxy-2(5H)-furanone in chlorinated drinking and humic waters. Chemosphere 15, 549–556.
RAPSON, W.H. et al. (1980). Mutagenicity produced by aqueous chlorination of organic compounds. Bull. Environm. Contam. Toxicol. 24, 590–596.
HOLMBOM, B. et al. (1987). Formation and properties of 3-chloro-4-(dichloro-methyl)-5-hydroxy-2(5H)-furanone, a potent mutagen in chlorinated waters. 6th Conference on Water Chlorination, Oak Ridge, Tennessee.
ONODERA, S. et al. (1986) Chemical changes of organic compounds in chlorinated water. J. Chromatography 360, 137–150.
MEIER, J.R. et al. (1983). Formation of mutagens following chlorination of humic acid; a model for mutagen formation during drinking water treatment. Mutation Res. 118, 25–41.
SUESSMUTH, R. (1982). Genetic effects of amino acids after chlorination. Mutation Res. 105, 23–28.
RAPSON, W.H. et al. (1985), Mutagenicity produced by aqueous chlorination of tyrosine. R.L.Jolley et al (eds.), Water Chlorination, Vol.5, Lewis Publ., 237–249.
Meier, J.R. et al (1985). Identification of mutagenic compounds formed during chlorination of humic acid. Mutation Res. 157, 111–122.
KRONBERG, L. et al (1987). Identification of the strong mutagen 3-chloro-4-(dichloromethyl)-5-hydroxy-2(5H)-furanone and of its geometric isomer E-2-chloro-3-(dichloromethyl)-4-oxo-butenoic acid in mutagenic fractions of chlorine treated humic water and in drinking water. 6th Conference on Water Chlorination, Oak Ridge, Tennessee.
STROEMBERG, L.M. et al (1987). An abundant chlorinated furanone in the spent chlorination liquor from pulp bleaching. Environ. Sci. Technol. 21, 754–756.
MEIER, J.R. et al. Studies on the potent bacterial mutagen, 3-chloro-4-(dichloromethyl)-5-hydroxy-2(5H)-furanone: aqueous stability, XAD recovery and analytical determination in drinking water and in chlorinated humic acid solutions. Mutation Res., in press.
KRONBERG, L. et al (1986). Determination of the strong mutagen, 3-chloro-4-(dichloromethyl)-5-hydroxy-2(5H)-furanone (MX) and identification of other chlorinated compounds present in mutagenic fractions of chlorinated humic water. A. Leonard and M. Kirsch-Volders (eds.), Proceedings of the XVIth Annual Meeting, European Environmental Mutagen Society, 104–107.
NORWOOD, D.L. et al (1987). Structural characterisation of aquatic humic material. Environ. Sci. Technol., 21, 791–798.
BACKLUND P et al (1985). Mutagenic activity in humic water treated with alternative disinfectants. H.A.M. de Kruijf and H.J. Kool (eds.), Organic Micropollutants in Drinking Water and Health. Elsevier, 257–264.
COGNET, L. et al (1986). Mutagenic activity of disinfection by-products. Environ. Health Persp. 69, 165–175.
LYKENS, B.W. et al (1986). Chemical products and toxicologic effects of disinfection. Jour. Amer. Wat. Wks. 78, 66–75.
MONARCO, S. et al (1983). Removal of mutagens from drinking water by granular activated carbon. Water Res. 9, 1015–1026.
CHEH, A.M. et al (1980). Nonvolatile mutagens in drinking water. Science 20, 90–92.
WILCOX, P. and DENNY, S. (1985). Effect of dechlorinating agents on the mutagenic activity of chlorinated water samples. R.L.Jolley et al (eds.), Water Chlorination, Vol.5, Lewis Publ. 1341–1353.
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© 1988 ECSC, EEC, EAEC, Brussels and Luxembourg
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Fielding, M., Horth, H. (1988). The Formation and Removal of Chemical Mutagens During Drinking Water Treatment. In: Angeletti, G., Bjørseth, A. (eds) Organic Micropollutants in the Aquatic Environment. Springer, Dordrecht. https://doi.org/10.1007/978-94-009-2989-0_37
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DOI: https://doi.org/10.1007/978-94-009-2989-0_37
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