Abstract
The special features of a new type of modulation fluorometer (PAM Fluorometer) are outlined in comparison to conventional measuring systems. Fluorescence is excited by 1 µs pulses of a light-emitting-diode and the pulse signal is processed via a selective window amplifier, yielding an exceptionally high dynamic range. The system is also capable of measuring P700 changes around 820 nm, using a modified emitter-detector unit. Due to its tolerance of large non-modulated background signals, the PAM Fluorometer is well suited for measurements in full sunlight and for quenching analysis by the saturation pulse method (determination of photochemical and non-photochemical quenching components). Photochemical quenching is shown to correspond closely to O2-evolution rate. However, as is examplified by the effect of ANT-2p, under special conditions an increase in photochemical quenching may go along with a decrease in quantum yield. Cyclic electron flow around PS II is suggested to be stimulated upon loss of PS II donor side capacity.
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References
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© 1988 Kluwer Academic Publishers
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Schreiber, U., Neubauer, C., Klughammer, C. (1988). New Ways of Assessing Photosynthetic Activity with a Pulse Modulation Fluorometer. In: Lichtenthaler, H.K. (eds) Applications of Chlorophyll Fluorescence in Photosynthesis Research, Stress Physiology, Hydrobiology and Remote Sensing. Springer, Dordrecht. https://doi.org/10.1007/978-94-009-2823-7_7
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DOI: https://doi.org/10.1007/978-94-009-2823-7_7
Publisher Name: Springer, Dordrecht
Print ISBN: 978-94-010-7771-2
Online ISBN: 978-94-009-2823-7
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