Abstract
The main advantages of measuring fluorescence emission and excitation spectra at low (77 K) instead of room (293 K) temperature are spectra with narrower and higher, better distinguishable bands, and the possibility to study rapid changes in the structure of photosynthetic apparatus. The sample for measuring correct fluorescence spectra must not contain a large amount of pigments (only pale thin leaves or chloroplast preparations are appropriate), must be properly mounted (chloroplasts on a translucent filter) and rapidly frozen, and not affected by substances causing degradation of pigment-protein complexes (not even mild detergents). Combination of emission and excitation spectra with their second derivatives and with calculated difference spectra enables to determine the presence of individual chlorophyll-protein complexes, their synthesis and degradation in thylakoids, chloroplasts and whole leaves.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Butko P and Szalay L, 1986. Effect of glycerol on the low-temperature fluorescence spectra of green algae. Acta Biochimica et Biophysica Hungarica 21: 391–395.
Butler WL and Strasser RJ, 1977. Does the rate of cooling affect fluorescence properties of chloroplasts at -196 °С Biochimica et Biophysica Acta 462: 283–289.
Govindjee, Amesz J and Fork DC, 1986. Light emission by plants and bacteria. Academic Press, Orlando — San Diego — New York — Austin — Boston — London — Sydney — Tokyo — Toronto.
Harnischfeger G, 1977. The use of fluorescence emission at 77 oK in the analysis of the photosynthetic apparatus of higher plants and algae. Advances in Botanical Research 5: 1–52.
Harnischfeger G, 1979. Connection between the rate of cooling and fluorescence properties at 77 K of isolated chloroplasts. Biochimica et Biophysica Acta 546: 348–355.
Lebedev NN, Šiffel P and Krasno vskiï AA, 1985. Detection of protochlorophyllide forms in irradiated green leaves and chloroplasts by difference fluorescence spectroscopy at 77 K. Photosynthetica 19: 183–187.
Lebedev NN, Šiffel P, Pakshina EV and Krasnovskiï AA, 1986. The effect of acidification on absorption and fluorescence spectra of French bean chloroplasts and kinetics of pheophytin formation. Photosynthetica 20: 124–130.
Meister A, 1977. Messung von Absorptionspektren in vivo. Kulturpflanze 25: 141–154.
Šesták Z and Šiffel P, 1988. Changes in chloroplast fluorescence during leaf development. This volume.
Šiffel P, Lebedev NN and Krasnovskiï AA, 1987. Detection of short-wavelength chlorophyll a emission in green leaves. Photosynthetica 21: 23–28.
Szalay L, Török M and Govindjee, 1967. Effect of secondary fluorescence on the emission spectrum and quantum yield of fluorescence in chlorophyll a solutions and algal suspensions. Acta Biochimica et Biophysica Academica Scientiarum Hungaricae 5: 261–262.
Weis E, 1985. Chlorophyll fluorescence at 77 K in intact leaves: Characterization of a technique to eliminate artifacts related to self-absorption. Photosynthesis Research 6: 73–86.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1988 Kluwer Academic Publishers
About this chapter
Cite this chapter
Šiffel, P., Šesták, Z. (1988). Low Temperature Fluorescence Spectra of Chloroplasts: Methodical Aspects and Possible Applications. In: Lichtenthaler, H.K. (eds) Applications of Chlorophyll Fluorescence in Photosynthesis Research, Stress Physiology, Hydrobiology and Remote Sensing. Springer, Dordrecht. https://doi.org/10.1007/978-94-009-2823-7_6
Download citation
DOI: https://doi.org/10.1007/978-94-009-2823-7_6
Publisher Name: Springer, Dordrecht
Print ISBN: 978-94-010-7771-2
Online ISBN: 978-94-009-2823-7
eBook Packages: Springer Book Archive