Photosystem II Heterogeneity in Chloroplasts

Melis, A.; Guenther, G. E.; Morrissey, P. J.; Ghirardi, M. L.

doi:10.1007/978-94-009-2823-7_4

A. Melis²,
G. E. Guenther²,
P. J. Morrissey² &
…
M. L. Ghirardi²

280 Accesses
5 Citations

Abstract

Two aspects of photosystem II (PSII) heterogeneity are discussed. (A) The PSII antenna heterogeneity refers to the existence of PSII centers with distinct photosynthetic unit size. Measurements of PSII antenna size in developing chloroplasts, in Chl b-less and Chl b-deficient mutants, and in mature higher plant chloroplasts indicated the existence of three distinct populations of PSII centers: PSII_α with a total of 210 (or more) chlorophyll (a+b) molecules; PSII_β with a total of 120 Chl (a+b) molecules. In the absence of Chl b, a PSII complex containing only 50 Chl a molecules (PSII-50) was identified. A developmental relationship among PSII-50, PSII_β and PSIIα is proposed. According to this hypothesis, the formation of the complete PSII unit involves the assembly of three modular complexes (PSII-50, LHC II-inner, and LHC II-peripheral). Addition of LHC II-inner to PSII-50 yields PSII_β. Addition of LHC II-peripheral to PSII_β yields PSII_α. The relative proportion of PSII centers with the three antenna configurations depends on the developmental stage of the chloroplast and on the availability of Chl b. Mature wild type chloroplasts contain PSIIα (75–80% of the total PSII) and PSII_β (20–25% of the total PSII). (B) The PSII reducing side heterogeneity refers to the existence of PSĪI centers with impaired Q to Q_B electron transfer interaction (Q_B-nonreducing centers). The fraction of PSII-Q_B-nonreducing centers is small (20–25% of the total PSII) as tested in several chloroplast preparations. This steady state concentration of Q_B-nonreducing centers appears independent of the developmental stage of the chloroplast and also independent of the PSII photosynthetic unit size. In mature spinach chloroplasts, PSII_β and PSII-Q_B-nonreducing centers constitute one and the same pool of PSII centers. It is proposed that PSII-Q_B-nonreducing centers represent newly synthesized and/or repaired PSII centers which have not yet established a functional interaction between Q_Ȧ and Q_B.

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Division of Molecular Plant Biology, University of California, 313 Hilgard Hall, Berkeley, CA, 94720, USA
A. Melis, G. E. Guenther, P. J. Morrissey & M. L. Ghirardi

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A. Melis
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G. E. Guenther
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P. J. Morrissey
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M. L. Ghirardi
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Botanisches Institut (Plant Physiology and Biochemistry), University of Karlsruhe, Karlsruhe, Germany
Hartmut K. Lichtenthaler

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Melis, A., Guenther, G.E., Morrissey, P.J., Ghirardi, M.L. (1988). Photosystem II Heterogeneity in Chloroplasts. In: Lichtenthaler, H.K. (eds) Applications of Chlorophyll Fluorescence in Photosynthesis Research, Stress Physiology, Hydrobiology and Remote Sensing. Springer, Dordrecht. https://doi.org/10.1007/978-94-009-2823-7_4

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DOI: https://doi.org/10.1007/978-94-009-2823-7_4
Publisher Name: Springer, Dordrecht
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