Abstract
When a photon strikes a chlorophyll molecule it becomes excited, an electron is raised from a ground state in the molecular orbital to an excited state. Blue photons, carrying higher energy, raise electrons to the excited singlet state 2, red photons raise electrons to excited state 1. These of course are the photons which are preferentially absorbed. Green photons are mostly not absorbed and it is the green light which is reflected (from and transmitted through) leaves which gives them their colour. Excited state 2 decays very rapidly to excited state 1 as electrons cascade down the energy gradient, losing energy by radiationless de-excitation. None of this energy is available for photosynthesis. It is the red, more long-lived, excited state created directly by a red photon (or indirectly by a blue photon) which is the starting point of photosynthesis and, therefore, of most of biological energy transduction. It is from here that electrons are transported through the two photosystems to reduce NADP and finally CO2. In addition the creation of the red excited state is accompanied by the generation of the massive oxidising potential, the positively charged “holes” within PSII, which are refilled by electrons drawn from water. Otto Warburg believed photosynthetic energy transduction to be perfect. It is not. Oxidation is inevitably associated with reduction. Electrons which have been raised to an excited state will fall back into the ground state if there is no acceptor available. Much of the energy dissipated in these circumstances is dissipated as heat. A fraction, released as electrons drop more or less directly back to the ground state, brings about the emission of photons.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Duysens, W.L.N.M. and Sweers, H.E. 1963 Mechanism of two photochemical, reactions in algae as studied by means of fluorescence. In: Studies on Microalgae and Photosynthetic Bacteria (Jap. Soc. of Plant Physiol., eds) Tokyo, University of Tokyo Press, pp. 353–372.
Horton, P. 1985 Interactions between electron transfer and carbon assimilation. In: Photos y nthetic Mechanisms and the Environment. (Barber, J. and Baker, N.R., eds). Topics in Photosynthesis Vol. 6. Elsevier Science Pubs., pp. 135–187.
Krause, G.H., and Weis, E. 1984 Review: Chlorophyll fluorescence as a tool in plant physiology. II. Interpretation of fluorescence signals. Photosynthesis Research, Vol 5. Martinus Nijhoff/Dr. W. Junk, The Hague, pp 139–157.
Lavorell, J. and Etienne, A.L. 1977 In vivo chlorophyll fluorescence. In: Primary Processes in photosynthesis (Barber, J., ed). Elsevier/North Holland Biomedical Press, Amsterdam pp 203–268.
Schreiber, U and Bilger, W 1986 Rapid assessment of stress effect on plant leaves by chlorophyll fluorescence measurements. NATO workshop Sesimbra Portugal Oct 1985. In press
Schreiber, U., Schliwa, U. and Bilger, W. 1986 Continuous recording of photochemical and non-photochemical chlorophyll fluorescence quenching with a new type of modulation fluorometer. Photosynthesis Research 10, 51–62.
Heber, U. 1969 Conformational changes of chloroplasts induced by illumination of leaves in vivo. Biochim. Biophys. Acta 180, 302–319.
Sivak, M.N. and Walker, D.A. 1984 New perspectives in the understanding of the regulation of photosynthesis and its relation to chlorophyll fluorescence kinetics through the study of oscillations. In: Oscillations in physiological systems: Dynamics and control (Proc. Symp., Oxford, September 1984) Inst, of Measurement and control, London, pp 91–96.
Sivak, M.N. and Walker, D.A. 1986 Photosynthesis in vivo can be limited by phosphate supply. New Phytol. 102, 499–512
Sivak, M.N., Heber, U. and Walker, D.A. 1985 Chlorophyll a fluorescence and light-scattering kinetics displayed by leaves during induction of photosynthesis. Planta 163, 419–423.
Sivak, M.N., Lea, P.J. and Walker, D.A. 1986 New developments in the measurement of photosynthesis in vivo. Implications for plant genetic engineering. Biochemical. Soc. Trans. 14, 63–64.
Walker, D.A. 1981 Secondary fluorescence kinetics of spinach leaves in relation to the onset of photosynthetic carbon assimilation. Planta 153, 273–278.
Walker, D.A. and Osmond, C.B. 1986 Measurement of photosynthesis in vivo using a leaf disc electrode: Correlations between light dependence of steady-state photosynthetic O2 evolution and chlorophyll a fluorescence transients. Proc. Roy. Soc. B 227, 267–280.
Walker, D.A. 1988 The use of the oxygen electrode and fluorescence probes in simple measurements of photosynthesis. Revised edition. Oxygraphics Limited, Sheffield, pp1–188
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1988 Kluwer Academic Publishers
About this chapter
Cite this chapter
Walker, D.A. (1988). Some Aspects of the Relationship between Chlorophyll a Fluorescence and Photosynthetic Carbon Assimilation. In: Lichtenthaler, H.K. (eds) Applications of Chlorophyll Fluorescence in Photosynthesis Research, Stress Physiology, Hydrobiology and Remote Sensing. Springer, Dordrecht. https://doi.org/10.1007/978-94-009-2823-7_2
Download citation
DOI: https://doi.org/10.1007/978-94-009-2823-7_2
Publisher Name: Springer, Dordrecht
Print ISBN: 978-94-010-7771-2
Online ISBN: 978-94-009-2823-7
eBook Packages: Springer Book Archive