Skip to main content

Abstract

A definition of stress to plants and a general stress concept are presented including a list of possible major natural and anthropogenic stress factors. It is shown that the white light or laser-induced chlorophyll fluorescence of predarkened leaves is a very suitable tool for early stress detection in plants as well as for regeneration studies. The different chlorophyll fluorescence parameters to be measured are: variable fluorescence (ratio fmax/fo), the vitality index (fluorescence decrease ratio, Rfd-values), the stress adaption index Ap, the fluorescence spectra (ratio F690/F735) and the photochemical Q- and non-photochemical E-quenching (qQ and qE). Some examples of the measurement of these parameters under stress conditions are given.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 129.00
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 169.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  • HAITZ M and LICHTENTHALER HK, 1988. The measurement of Rfd-values as plant vitality index with the portable field fluororneter and the PAM-fluorometer. In: Applications of Chlorophyll Fluorescence, Lichtenthaler HK ed., pp.233–238, Kluwer Academic Publishers, Dordrecht (this volume).

    Google Scholar 

  • KOSCANYI L, HAITZ M and LICHTENTHALER HK, 1988. Measurement of laser-induced chlorophyll fluorescence kinetics using a fast acoustooptic device. In: Applications of Chlorophyll Fluorescence, LICHTENTHALER HK ed., pp. 91–99, Kluwer Academic Publishers Dordrecht (this volume).

    Google Scholar 

  • KRAUSE GH and WEIS E, 1984. Chlorophyll fluorescence as a tool in plant physiology. II. Interpretation of fluorescence signals, Photosynth. Res. 5: 139–157.

    Article  CAS  PubMed  Google Scholar 

  • LARCHER W, 1987. Stress bei Pflanzen. Naturwissenschaften 74: 158–167.

    Article  CAS  Google Scholar 

  • LEVITT J, 1980. Responses of plants to environmental stresses. Vol. 1, Academic Press, New York.

    Google Scholar 

  • LICHTENTHALER HK, 1984. Influence of environmental factors on composition and function of the photosynthetic apparatus. In: Advances in Photosynthesis Research, Sybesma C ed., Vol. IV, pp. 241–244, Martinus Nijhoff/Dr.W. Junk Publisher, The Hague.

    Chapter  Google Scholar 

  • LICHTENTHALER HK, 1986. Laser-induced chlorophyll fluorescence of living plants. In: Proceed. Remote Sensing Sensing Symp. IGARSS Zürich Vol. III, pp. 1587–1590, ESA Publications Division, Noordwijk.

    Google Scholar 

  • LICHTENTHALER HK, 1987. Chlorophyll fluorescence signatures of leaves during the autumnal chlorophyll breakdown. J. Plant Physiol. 131: 101–110.

    Article  CAS  Google Scholar 

  • LICHTENTHALER HK and BUSCHMANN C, 1984a. Das Waldsterben aus botanischer Sicht, G. Braun Verlag Karlsruhe.

    Google Scholar 

  • LICHTENTHALER HK and BUSCHMANN C, 1984b. Photooxidative changes in pigment composition and photosynthetic activity of air-polluted spruce needles (Picea abies L.). In Advances in Photosynthesis Research Vol. IV, Sybesma C ed., pp. 245–250, Martinus Nijhoff Publisher, The Hague.

    Chapter  Google Scholar 

  • LICHTENTHALER HK and RINDERLE U, 1988. The role of chlorophyll fluorescence in the detection of stress conditions in plants. CRC Critical Reviews in Analytical Chemistry 19, Suppl. I: S 29–S 85.

    Google Scholar 

  • LICHTENTHALER HK, BUSCHMANN C, DOLL M, FIETZ H-J, BACH T, KOZEL U, MEIER D and RAHMSDORF U, 1981. Photosynthetic activity, chloroplast ultrastructure, and leaf characteristics of high-light and low-light plants and of sun and shade leaves. Photosynthesis Res. 2: 115–141.

    Article  CAS  Google Scholar 

  • LICHTENTHALER HK, KUHN G, PRENZEL U, BUSCHMANN C and MEIER D, 1982. Adaptation of chloroplast-ultrastructure and of chlorophyl1-protein levels to high-light and low-light growth conditions. Z. Naturforsch. 37c: 464–475.

    Article  CAS  Google Scholar 

  • LICHTENTHALER HK, MEIER D and BUSCHMANN C, 1984. Development of chloroplasts at high and low quanta fluence rates. Israel J. Botany 33: 185–194.

    CAS  Google Scholar 

  • LICHTENTHALER HK, SCHMUCK G, DOLL M and BUSCHMANN C, 1985. Untersuchungen über die Funktionsfähigkeit des Photosyntheseapparates bei Nadeln gesunder und geschädigter Koniferen. In: PEF Bericht KfK-PEF2, pp. 81–105, Kernforschungszentrum, Karlsruhe.

    Google Scholar 

  • LICHTENTHALER HK, BUSCHMANN C, RINDERLE U and SCHMUCK G, 1986. Application of chlorophyll fluorescence in ecophysiology. Radiation Environmental Biophysics 25: 297–308.

    Article  CAS  PubMed  Google Scholar 

  • LICHTENTHALER HK, RINDERLE U, KRITIKOS G and ROCK B, 1987. Classification of damaged spruce stands in the Northern Black Forest by airborne reflectance and terrestrial chlorophyll fluorescence measurements. In: 2nd DFVLR Status Seminar: Untersuchung und Kartierung von Waldschäden mit Methoden der Fernerkundung pp. 228–252, DFVLR Oberpfaffenhofen/München.

    Google Scholar 

  • MURATA N and SATOH K, 1986. Absorption and fluorescence emission by intact cells, chloroplasts, and chlorophyll-protein complexes. In: Light Emission by Plants and Bacteria. Govindjee, Amesz N and Fork DC eds., pp. 137–159, Academic Press Inc., Orlando.

    Chapter  Google Scholar 

  • RINDERLE U and LICHTENTHALER HK, 1988. The chlorophyll fluorescence ratio F690/F735 as a possible stress indicator. In: Applications of Chlorophyll Fluorescence, Lichtenthaler HK ed., pp. 176–183, Kluwer Academic Press, Dordrecht (this volume).

    Google Scholar 

  • ROCK BN, HOSHIZAKI T, LICHTENTHALER HK and G SCHMUCK, 1986. Comparision of in situ spectral measurements of forest decline symptoms in Vermont (USA) and the Schwarzwald (FRG). In: Proc. Internat. Geoscience Remote Sensing Symposium, IGARSS Zürich, Vol. III, pp. 1667–1672, ESA Publications Division, Noordwijk.

    Google Scholar 

  • SCHREIBER U, SCHLIWA U and BILGER W, 1986. Continuous recording of photochemical and non-photochemical chlorophyll fluorescence quenching with a new type of modulation fluorometer. Photosynth. Res. 10:51–62.

    Article  CAS  PubMed  Google Scholar 

  • SCHMUCK G and LICHTENTHALER HK, 1986. Application of laser-induced chlorophyll a fluorescence in the forest decline research. In: Proc. Internat. Geoscience and Remote Sensing Symposium, Igarss Zürich, Vol. III, pp. 1587–1590, ESA Publications Division, Noordwijk.

    Google Scholar 

  • ŠIFFEL LP and ŠESTáK Z, 1988. Low temperature fluorescence spectra of chloroplasts: methodical aspects and possible applications. In: Applications of Chlorophyll Fluorescence, Lichtenthaler HK ed., pp. 50–56, Kluwer Academic Press, Dordrecht (this volume).

    Google Scholar 

  • STRASSER RJ, 1986. Laser-induced fluorescence of plants and its application to environmental research. In: Proc. Internat. Geoscience Remote Sensing Symposium, Igarss Zürich, Vol. III, pp. 1581–1585, ESA Publications Division, Noordwijk.

    Google Scholar 

  • STRASSER R, SCHWARZ B and BUCHER J, 1987. Simultane Messung der Chlorophyll-Fluoreszenz Kinetik bei verschiedenen Wellenlängen als rasches Verfahren zur Frühdiagnose von Immissionsbelastungen an Waldbäumen. Ozoneinwirkung auf Buchen und Pappeln. Europ. J. Forest Pathology 17: 149–157.

    Article  CAS  Google Scholar 

  • WEIS E, 1984. Short term acclimation of spinach to high temperatures. Effect on chlorophyll fluorescence at 293 and 77 Kelvin in intact leaves. Plant Physiol. 74:402–407.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • WHITMORE EM and FREER-SMITH PH, 1982. Growth effects of SO2. and/or NO2 on woody plants and grasses during spring and summer. Nature 300: 55–57.

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 1988 Kluwer Academic Publishers

About this chapter

Cite this chapter

Lichtenthaler, H.K. (1988). In Vivo Chlorophyll Fluorescence as a Tool for Stress Detection in Plants. In: Lichtenthaler, H.K. (eds) Applications of Chlorophyll Fluorescence in Photosynthesis Research, Stress Physiology, Hydrobiology and Remote Sensing. Springer, Dordrecht. https://doi.org/10.1007/978-94-009-2823-7_16

Download citation

  • DOI: https://doi.org/10.1007/978-94-009-2823-7_16

  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-94-010-7771-2

  • Online ISBN: 978-94-009-2823-7

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics