Uniparental, Fusant, and Chimeric Plants Regenerated from Protoplasts after Streak Plating in Agarose Gels

Abstract

Some results are discussed, here, which were obtained through plating of protoplasts in streaks of locally high density embedded in 10 μl agarose media droplets (culture lenses) covered with liquid media (FIGURE 1; Binding and Kollmann 1985). This technique is a modification of the common agarose plating method first introduced by Shepard 1980. Advantages of the streaky plating technique appeared in three areas namely in the regeneration of protoplasts, the fusion of protoplasts and the production of cell graft chimeras (Binding et al., in press).