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Research Instrumentation for the 21st Century pp 63–88Cite as

Flow sorting of cells and organelles

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Part of the book series: Beltsville Symposia in Agricultural Research ((BSAR,volume 11))

Abstract

Flow techniques offer the possibility of rapidly and precisely determining many characteristics of each member of a population, and of efficiently separating desired subpopulations for detailed study or culture. Measurements on objects can be made based on their electrical and acoustical properties, light scatter, uptake of specific fluorescent dyes, affinity for antibodies, and binding of nucleic acid probes. Asymmetrical objects can be oriented in flow and scanned or imaged to obtain morphological information. Spatial resolution below 1 µm has been achieved. Measurement rates are limited only by problems of coincidence between objects; operation above 105/sec has been achieved. High efficiency, high purity sorting at rates above 2 × 104/sec has been demonstrated. The detection threshold for fluorochromes is determined primarily by autofluorescence of the objects being measured.

In the future we are likely to see improved handling of multiparameter data (measurements of 4 or more characteristics of each object are not unusual), increased application to the measurement of functional states of cells, greater emphasis on kinetic measurements on time scales ranging down to milliseconds, increased use of antibodies and nucleic acid sequences for specific labeling, and development of rugged, inexpensive instrumentation for clinical and field use.

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Authors and Affiliations

  1. Lawrence Livermore National Laboratory, Biomedical Sciences Division, University of California, P.O. Box 5507, L-452, Livermore, CA, 94550, USA

    Daniel Pinkel

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  1. Daniel Pinkel
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Gary R. Beecher

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© 1988 Martinus Nijhoff Publishers, Dordrecht

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Pinkel, D. (1988). Flow sorting of cells and organelles. In: Beecher, G.R. (eds) Research Instrumentation for the 21st Century. Beltsville Symposia in Agricultural Research, vol 11. Springer, Dordrecht. https://doi.org/10.1007/978-94-009-2748-3_4

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  • DOI: https://doi.org/10.1007/978-94-009-2748-3_4

  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-94-010-7734-7

  • Online ISBN: 978-94-009-2748-3

  • eBook Packages: Springer Book Archive

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