Abstract
Time course of the growth of cyclomaltodextrin glucanotransferase (CGTase)-producing Bacillus circulans var. alkalophilus was studied in detail in a standard shaking-flask cultivation. The growth curve was biphasic and during the first phase of it the pH sharply dropped by 1.3–1.5 units. Most of the total carbohydrates disappeared distinctly prior to the bulk growth and simultaneously a transient peak of reducing sugars with saccharifying and liquefying amylolytic activities were found but only traces of CGTase. During the active cell growth the pH recovered by about 0.5 units and till the stationary phase around 65% of CGTase was excreted into the medium. The CGTase activity increased by about 20% in the stationary phase and the rest of CGTase released slowly during the death phase.
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© 1988 Kluwer Academic Publishers
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Mäkelä, M.J., Paavilainen, S.K., Korpela, T.K. (1988). Cultural Characteristics of an Cyclomaltodextrin Glucano-Transferase-Producing Alkalophilic Bacillus Sp.. In: Huber, O., Szejtli, J. (eds) Proceedings of the Fourth International Symposium on Cyclodextrins. Advances in Inclusion Science, vol 5. Springer, Dordrecht. https://doi.org/10.1007/978-94-009-2637-0_4
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DOI: https://doi.org/10.1007/978-94-009-2637-0_4
Publisher Name: Springer, Dordrecht
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