Abstract
A large number of alkalophilic bacteria isolated from soil was screened for cyclodextrin glycosyltransferase activity. The strain 1-1 identified as a member of the Bacillus firmus/lentus group was shown to secrete a cyclodextrin glycosyltransferase which forms mainly β-cyclodextrin, small amounts of γ-cyclodextrin and no α-cyclodextrin. The gene for this enzyme was cloned in E. coli and the nucleotide sequence was determined. The nucleotide sequence reveals an open reading frame of 2109 bp, corresponding to a protein of 703 amino acids (aa). A signal peptide precedes the native polypeptide which differs from all known analogous structures by a negative net charge in its N-terminal part.
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© 1988 Kluwer Academic Publishers
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Schmid, G., Englbrecht, A., Schmid, D. (1988). Cloning and Nucleotide Sequence of a Cyclodextrin Glycosyltransferase Gene from the Alkalophilic Bacillus 1-1. In: Huber, O., Szejtli, J. (eds) Proceedings of the Fourth International Symposium on Cyclodextrins. Advances in Inclusion Science, vol 5. Springer, Dordrecht. https://doi.org/10.1007/978-94-009-2637-0_11
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DOI: https://doi.org/10.1007/978-94-009-2637-0_11
Publisher Name: Springer, Dordrecht
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