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Methods to Study Ras Oncogene-Mediated Induction of the Metastatic Phenotype

  • Chapter
Mechanisms of Carcinogenesis

Part of the book series: Cancer Growth and Progression ((CAGP,volume 2))

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Abstract

Acquisition of the metastatic potential involves participation of various cellular properties, in addition to those required for the transforming process. The metastatic cells must depart from the primary tumor, invade surrounding matrix, intravasate lymphatics and blood vessels, survive mechanical trauma of the circulation, resist cytotoxic immune cells, extravasate, and finally proliferate in a distant organ. Considerable progress has been made in identifying different enzymatic and surface properties of tumor cells that are associated with the metastatic phenotype. However, with the techniques available in the past to study the genetic aspect of metastasis no specific genetic determinants have been found. Most studies have involved assessing the inheritability of the overall metastatic phenotype, using somatic cell hybridization technique where normal cells and tumor cells, or nonmetastatic and metastatic cells have been fused. Most of the cell hybridization studies have demonstrated that both the tumorigenic and the metastatic phenotypes behave as recessive traits (28, 44, 56).

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Authors
  1. U. P. Thorgeirsson
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  2. T. Turpeenniemi-Hujanen
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  3. M. Ballin
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  4. L. A. Liotta
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Editors and Affiliations

  1. National Cancer Institute, National Institutes of Health, Bethesda, Md., USA

    Elizabeth K. Weisburger

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© 1989 Kluwer Academic Publishers, Dordrecht

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Thorgeirsson, U.P., Turpeenniemi-Hujanen, T., Ballin, M., Liotta, L.A. (1989). Methods to Study Ras Oncogene-Mediated Induction of the Metastatic Phenotype. In: Weisburger, E.K. (eds) Mechanisms of Carcinogenesis. Cancer Growth and Progression, vol 2. Springer, Dordrecht. https://doi.org/10.1007/978-94-009-2526-7_14

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  • DOI: https://doi.org/10.1007/978-94-009-2526-7_14

  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-94-010-7641-8

  • Online ISBN: 978-94-009-2526-7

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