Abstract
Isolated chloroplasts show substantial rates of protein synthesis when illuminated. This ‘in organello’ protein synthesis system has been advantageously utilised to elucidate the coding capacity of chloroplast and the regulation of chloroplast genes. The system is also being used recently to transcribe and translate homologous and heterologous templates. In this mini-review, we attempt to critically evaluate the available literature and present the current and the prospective lines of research.
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References
Alscher R, Patterson R and Jagendorf AT (1978) Activity of thylakoid-bound ribosomes in pea chloroplasts. Plant Physiol 62: 88–93
Alscher-Herman R, Jagendorf AT and Grumet R (1979) Ribosome-thylakoid association in peas. Influence of anoxia. Plant Physiol 64: 232–235
Ananda Krishnan V and Gnanam A (1988) Properties and regulation of Mg2+ dependent chloroplast inorganic pyrophosphatase from Sorghum vulgare leaves. Arch Biochem Biophys 260: 277–284
Archer EK, Hakanssan G and Bonnet HT (1987) The phenotype of a virescent chloroplast mutant in tobacco is associated with the absence of a 37.5 kD thylakoid Polypeptide. Plant Physiol 83: 926–932
Arnon DI and Chain RK (1975) Regulation of ferredoxin-catalysed photosynthetic phosphorylation. Proc Natl Acad Sci USA 72: 4961–4965
Bard J, Bourque DP, Hildebrand M and Zaitlin D (1985) In vitro expression of chloroplast genes in lysates of higher plant chloroplasts. Proc Natl Acad Sci USA 82: 3983–3987
Barraclough R and Ellis RJ (1980) Protein synthesis in chloroplasts. IX. Assembly of newly synthesized large subunits into ribulose bisphosphate carboxylase in isolated intact pea chloroplasts. Biochim Biophys Acta 608; 19–31
Bhaya D and Jagendorf AT (1984a) Optimal conditions for translation by thylakoid-bound polysomes from pea chloroplasts. Plant Physiol 75: 832–838
Bhaya D and Jagendorf AT (1984b) Syunthesis or subunit III of CF0 by thylakoid bound polysomes from pea chloroplasts. Plant Mol Biol 3(5): 277–280
Bhaya D and Jagendorf AT (1985) Synthesis of the xx and subunits or coupling factor 1 by polysomes from pea chloroplasts. Arch Biochem Biophys 237: 217–223
Blair GE and Ellis RJ (1973) Protein synthesis in chloroplasts. 1. Light-driven synthesis of the large subunit of fraction 1 protein by isolated pea chloroplasts. Biochim Biophys Acta 319: 223–234
Bloom MV, Miles P and Roy H (1983) Light dependent assembly of ribulose-1,5-biphosphate carboxylase. Proc Natl Acad Sci USA 80: 1013–1017
Bottomley W and Whitfield P (1979) Cell-free transcription and translation of total spinach chloroplast DNA. Eur J Biochem 93: 31–39
Bottomely W, Spencer D and Whitfeld PR (1974) Protein synthesis in isolated chloroplasts. Comparison of light driven and ATP-driven synthesis. Arch Biochem Biophys 164: 106–117
Bouthyette P-Y and Jagendorf AT (1981) Calcium inhibition of amino acid incorporation by pea chloroplasts and the question of loss of activity with age. In: Akoyunoglou G (ed.) Photosynthesis V Chloroplast Development, pp 599–609. Philadelphia: Balaban Intern Science Services
Bricker TM and Newman DW (1980) Quantitative changes in the chloroplast thylakoid Polypeptide complement during senescence. Z Pflanzenphysiol 98: 339–346
Camerino G, Sai A and Ciferri O (1982) A chloroplast system capable of translating heterologous mRNAs. FEBS Lett 150(1): 94–98
Chen JL and Wildman SG (1966) Functional chloroplast polyribosomes from tobacco leaves. Science 155: 1271–1273
Chen JL and Wildman SG (1970) ‘Free’ and membrane bound ribosomes, and nature of products formed by isolated tobacco chloroplasts incubated for protein synthesis. Biochim Biophys Acta 209: 207–219
Chua N-H, Blobel G, Siekevitz P and Palade GE (1973) Attachment of chloroplast polysomes to thylakoid membranes in Chlamydomonas reinhardtii. Proc Natl Acad Sci USA 70: 1554–1558
Ciferri O, DiPasquale G and Tiboni O (1979) Chloroplast elongation factors are synthesized in the chloroplasts. Eur J Biochem 102: 331–335
Clark MF, Mathews REF and Ralph RK (1964) Ribosomes and polyribosomes in Brassica pekinsis. Biochim Biophys Acta 91: 289–304
Cohen BN, Bloom MV, Coleman T and Weissbach H (1984) Analysis of an in vitro chloroplasts gene expression using a simplified dipeptide synthesis system. In: Cardinale GJ (ed.) Annual Report 1983, Roche Institute of Molecular Biology, pp 77–78
Colijn CM, Kool AJ and Nijkamp HJJ (1982) Protein synthesis in Petunia hybrida chloroplasts isolated from leaves and cell cultures. Planta 155: 37–44
Cushman JC and Price CA (1986) Synthesis and turnover of proteins in proplastids and chloroplasts of Euglena gracilis. Plant Physiol 82(4): 972–977
Daniell H, Krishnan M, Uma Bai V and Gnanam A (1986) An efficient and prolonged in vitro translational system from isolated cucumber Cucumis sativus etioplasts. Biochem Biophys Res Commun 135(1): 248–255
Dockerty A and Merrett MJ (1979) Isolation and enzymic characterization of Euglena chloroplasts. Plant Physiol 63: 468–473
Doherty A and Gray JC (1979) Synthesis of cytochrome f by isolated pea chloroplasts. Eur J Biochem 98: 87–92
Doherty A and Gray JC (1980) Synthesis of a dicyclohexylcarbodimide binding proteolipid by isolated pea chloroplasts. Eur J Biochem 108: 131–136
Dorne A-M, Lescure A-M and Mache R (1984) Site of synthesis of spinach chloroplast ribosomal proteins and formation of incomplete ribosomal particles in isolated chloroplasts. Plant Mol Biol 3: 83–90
Drumm HE and Margulies MM (1970) In vitro protein synthesis by plastids of Phaseolus vulgaris. IV. Amino acid incorporation by etioplasts and effect of illumination of leaves on incorporation by plastids. Plant Physiol 45: 435–442
Ellis RJ (1976) The intact chloroplast. In: Barber J (ed.) Protein and Nucleic Acid Synthesis by Chloroplasts, pp 335–364. The Netherlands: Elsevier/North-Holland Biomedical Press
Ellis RJ (1977) Protein synthesis by isolated chloroplasts. Biochim Biophys Acta 463: 185–215
Ellis RJ (1981) Chloroplast proteins synthesis transport and assembly. Ann Rev Plant Physiol 32: 111–138
Ellis RJ and Barraclough R (1978) Synthesis and transport of chloroplast proteins inside and outside the cell. In: Akoyunoglou G, Argyroudi-Akoyunoglou JH (eds) Chloroplast Development, pp 185–194. Amsterdam: Elsever/North-Holland Biomedical Press
Ellis RJ and Hartley MR (1982) Preparation of higher plant chloroplasts active in protein and RNA synthesis. In: Edelman M, Hallick RB and Chua N-H (eds) Methods in Chloroplast Molecular Biology, pp 170–188. Amsterdam: Elsevier Biomedical Press
Ellis RJ, Highfeld PE and Silverthrone J (1978) The synthesis of chloroplast proteins by subcellular systems. In: Hall DO, Coombs J and Goodwin TW (eds) Photosynthesis II — Proceedings of the IV International Congress on Photosynthesis, pp 497–506. London: The Biochemical Society
Ellis RJ and Van der Vies SM (1988) The Rubisco subunit binding protein. Photosynth Res 16: 000–000
Fish LE and Jagendorf AT (1982) High rates of protein synthesis by isolated chloroplasts. Plant Physiol 70(4): 1107–1114
Fish LE, Deshaies R and Jagendorf AT (1983) A Mg2+ requirement for rapid ATP-driven protein synthesis by intact pea chloroplasts. Plant Sci Lett 31: 139–146
Garcia S, Martin M and Sabater B (1983) Protein synthesis by chloroplasts during the senescence of barley leaves. Physiol Plant 57: 260–266
Geetha V and Gnanam A (1980a) An in vitro protein synthesizing system with isolated chloroplasts of Sorghum vulgare: An alternate assay system for exogenous template RNA. J Biol Chem 255(2): 492–497
Geetha V and Gnanam A (1980b) Identification of P700 chlorophyll a protein complex as a product of chloroplast protein synthesis. FEBS Lett 111(2): 272–276
Geetha V, Mohamed AH and Gnanam A (1980) Cell-free synthesis of active ribulose-1,5-bisphosphate carboxylase in the mesophyll chloroplasts of Sorghum vulgare. Biochim Biophys Acta 606(1): 83–94
Gnanam A, Mariappan T and Manjula Devi JD (1981) In vitro translation of exogenous messengers with isolated chloroplasts of spinach. In: Akoyunoglou G (ed.) Photosynthesis V. Chloroplast Development, pp 841–846, Philadelphia: Balban International Science Services
Goffeau A and Brachet J (1965) DNA-dependent incorporation of amino acids into the proteins of chloroplasts isolated from anucleate Acetabularia fragments. Biochim Biophys Acta 95: 302–313
Gomez Silva B and Schiff JA (1985) The light requirement for protein synthesis and carbon dioxide fixation in highly purified intact Euglena chloroplasts. Plant Science 39: 111–119
Grebanier AE, Coen DM, Rich A and Bogorad L (1978) Membrane proteins synthesized but not processed by isolated maize chloroplasts. J Cell Biol 78: 734–746
Green BR (1980) Protein synthesis by isolated Acetabularia chloroplasts. In vitro synthesis of the apoprotein of the P700 chlorophyll a protein complex (CPI). Biochim Biophys Acta 609: 107–120
Green BR (1982) Protein synthesis by isolated Acetabularia chloroplasts synthesis of the two minor chlorophyll a complexes in vitro. Eur J Biochem 128: 543–546
Hachtel W (1982) Biosynthesis and assembly of thylakoid membrane proteins in isolated chloroplasts from Vicia faba. The P700 chlorophyll a protein. Z Pflanzenphysiol 107(5): 383–394
Hachtel W (1985) Biosynthesis and assembly of chloroplast ribosomal proteins in the isolated chloroplasts from Vicia faba. Biochem Physiol Pflanz 180(2): 115–124
Hachtel W (1987) Synthesis and assembly of thylakoid membrane proteins in isolated pea chloroplasts. The chlorophyll a proteins of the photosystem II reaction center. Plant Sci 48(1): 43–48
Hartley MR, Wheeler AM and Ellis RJ (1975) Protein synthesis in chloroplasts. V. Translation of messenger RNA for the large subunit of Fraction I Protein in a heterologous cell-free system. J Mol Biol 91: 67–77
Hattori T and Margulies MM (1986) Synthesis of large subunit of ribulose bisphosphate carboxylase by thylakoid-bound polyribosomes from spinach, Spinacia oleracea cultivar savoy-supreme chloroplasts. Arch Biochem Biophys 244(2): 630–640
Herrin D and Michaels A (1985a) In vitro synthesis and assembly of the peripheral subunits of coupling factor CF, (α and β) by thylakoid bound ribosomes. Arch Biochem Biophys 237: 224–236
Herrin D and Michaels A (1985b) The chloroplast 32 kD protein is synthesized on thylakoid bound ribosomes in Chlamydomonas reinhardii. FEBS Lett 184(1): 90–95
Herrin D, Michaels A and Hickey E (1981) Synthesis of a chloroplast membrane Polypeptide on thylakoid bound ribosomes during the cell cycle of Chlamydomonas reinhardii 137+. Biochim Biophys Acta 655: 136–145
Hoffman-Falk H, Mattoo AK, Marder JB, Edelman M and Ellis RJ (1983) General occurrence and structural similarity of the rapidly synthesized 32000 dalton protein of the chloroplast membrane. J Biol Chem 257: 4583–4587
Hosier JP and Yocum CF (1987) Regulation of cyclic phosphorylation during ferredoxin mediated electron transport. Effect of DCMU and the NADPH/NADP+ ratio. Plant Physiol 83: 965–969
Hurewitz J and Jagendorf AT (1987) Further characterization of ribosome binding to thylakoid membranes. Plant Physiol 84: 31–34
Indira GM and Gnanam A (1976) Induction of chloroplast development-translation studies with temporally distinguished messengers. In: Bogorad L and Weil JH (eds) Acids Nucleiques et Synthese des Proteines Chez les Vegetaux, pp 617–621. Strasbourg: Colloq Int Cent Natl Rech Sci
Izawa S (1968) Effect of Hill reaction inhibitors on photosystem I. In: Shibata K, Takamiya A, Jagendorf AT and Fuller RC (eds) Comparative Biochemistry and Biophysics of Photosynthesis, pp 140–147. Pennsylvania: University Press, State College
Joy KW and Ellis RJ (1975) Protein synthesis in chloroplasts IV. Polypeptides of the chloroplast envelope. Biochim Biophys Acta 378: 143–151
Kirk JTO (1970) Biochemical aspects of chloroplast development. Ann Rev Plant Physiol 21: 11–42
Klein RR and Mullet JE (1986) Regulation of chloroplast encoded chlorophyll-binding protein translation during higher plant chloroplast biogenesis. J Biol Chem 261(24): 11138–11145
Klein RR and Mullet JE (1987) Control of gene expression during higher plant chloroplast biogenesis. Protein synthesis and transcript levels of psb A, psa A — psa B and rbcL in dark grown and illuminated barley seedlings. J Biol Chem 262(9): 4341–4348
Kloppstech K, Ohad I and Schweizer HG (1986) Evidence for an extranuclear coding site for a heat shock protein in Acetabularia. Eur J Cell Biol 42: 239–245
Krishnan M, Krishnasamy S, Mannar Mannan R and Gnanam A (1987) In vitro synthesis of heat shock proteins by chloroplasts. In: Biggins J (ed.) Progress in Photosynthesis Research, Vol IV, pp 593–596. Dordrecht: Martinus Nijhoff
Krishnasamy S, Mannan RM, Krishnan M and Gnanam A (1988) Heat shock response of chloroplast genome in Vigna sinensis. J Biol Chem 263: 5104–5109
Kyle DJ (1985) The 32000 dalton QB protein of photosystem II. Photochem Photobiol 41: 107–116
Langridge P (1981) Synthesis of the large subunit of spinach ribulose bisphosphate carboxylase may involve a precursor Polypeptide. FEBS Lett 123: 85–89
Leu S, Mendiola-Morgenthaler L and Boschetti A (1984a) Protein synthesis by isolated chloroplasts (ed.) Advances in Photosynthetic Research IV, pp 541–544. The Hague: Martinus-Nijhoff/Dr W Junk Publishers
Leu S, Bolli R, Mendiola-Morgenthaler L and Boschetti A (1984b) In vitro translation of different messenger RNA containing fractions of Chlamydomonas reinhardii chloroplasts. Planta 160(3): 204–211
Liu X-Q and Jagendorf AT (1984) ATP-dependent proteolysis in pea chloroplasts. FEBS Lett 166: 248–252
Liu X-Q and Jagendorf AT (1985) Roles for ATP-dependent and ATP-independent proteases of pea chloroplasts in regulation of the plastid translation products. Physiol Veg 23: 749–755
Lyttleton JW (1962) Isolation of ribosomes from spinach chloroplasts. Exp Cell Res 26: 312–317
Malek L, Bogorad L, Ayers AR and Goldberg AL (1984) Newly synthesized proteins are degraded by an ATP-stimulated proteolytic process in isolated pea chloroplasts. FEBS Lett 166: 253–257
Mannan RM, Krishnan M and Gnanam A (1987) Synthesis of Polypeptides associated with PS I by the isolated Sorghum vulgare chloroplasts. In: Biggins J (ed.) Progress in Photosynthesis Research, Vol. IV, pp 589–592. Dordrecht: Martinus Nijhoff Publishers
Marder JB, Goloubinoff P and Edelman M (1984) Molecular architecture of the rapidly metabolized 32 Kilodalton protein of photosystem II. J Biol Chem 259: 3900–3908
Margulies MM (1986) Compartmentation of protein synthesis within the chloroplast. In: Akoyunoglou G (ed.) Regulation of Chloroplast Differentiation, pp 171–180. New York: Alan R Liss
Margulies MM and Michaels A (1974) Ribosomes bound to chloroplast membranes in Chlamydomonas reinhardii. J Cell Biol 60: 65–77
Margulies MM, Tiffany HL and Michaels A (1975) Vectorial discharge of nascent Polypeptides attached to chloroplast thylakoid membranes. Biochem Biophys Res Commun 64: 735–739
Margulies MM, Tiffany HL and Hattori T (1987) Photosystem I reaction center Polypeptides of spinach are synthesized on thylakoid bound ribosomes. Arch Biochem Biophys 254(2): 454–461
Mariappan T (1984) Protein synthesis in the chloroplasts. Ph.D. Thesis, Madurai Kamaraj University
Mendiola-Morgenthaler LR, Morgenthaler JJ and Price CA (1976) Synthesis of coupling factor CF1 protein by isolated spinach chloroplasts. FEBS Lett 62: 96–100
Miller ME and Price CA (1982) Protein synthesis by developing plastids from Euglena gracilis. FEBS Lett 147: 156–160
Miller ME, Jurgenson JE, Reardon EM and Price CA (1983) Plastid translation in organello and in vitro during light induced development in Euglena. J Biol Chem 258: 14478–14484
Minami El and Watanabe A (1984) Thylakoid membranes the translation site of chloroplast DNA-regulated thylakoid Polypeptides. Arch Biochem Biophys 235(2): 562–570
Minami El and Watanabe A (1985) Detection of a precursor Polypeptide of the rapidly synthesized 32000 dalton thylakoid protein in spinach chloroplasts. Plant Cell Physiol 26(5): 839–846
Minami El, Shinohara K, Kuwabara T and Watanabe A (1986) In vitro synthesis and assembly of photosystem II proteins of spinach chloroplasts. Arch Biochem Biophys 244(2): 517–527
Morgenthaler JJ and Mediola-Morgenthaler L (1976) Synthesis of soluble thylakoid and envelope membrane proteins by spinach chloroplasts purified from gradients. Arch Biochem Biophys 172: 51–58
Mullet JE, Klein RR and Grossman AR (1986) Optimization of protein synthesis in isolated higher plant chloroplasts. Identification of paused translation intermediates. Eur J Biochem 155(2): 331–338
Nelson N, Nelson H and Schatz G (1980) Biosynthesis and assembly of the proton-translocating adenosine triphosphatase complex from chloroplasts. Proc Natl Acad Sci USA 77: 1361–1364
Nivison HT and Jagendorf AT (1984) Factors permitting prolonged translation by isolated pea Pisum sativum cultivar progress — No. 9 chloroplasts. Plant Physiol 75(4): 1001–1008
Obokata J (1984) Protein synthesis in isolated plastids during chloroplast development in wheat. Plant Cell Physiol 25(5): 821–830
Ohyama K, Fukuzawa H, Kockli T, Shirai H, Sano T, Sano S, Umesono K, Shiki Y, Takeuchi M, Chang Z, Aota S-I, Inokuchi H and Ozeki H (1986) Chloroplast gene organization deduced from complete sequence of liverwort Marchantia polymorplea chloroplast DNA. Nature 322: 572–574
Ortiz W, Reardon EM and Price CA (1980) Preparation of chloroplasts from Euglena highly active in protein synthesis. Plant Physiol 66: 291–294
Ramanujam P, Gnanam A and Bose S (1981) Stimulation of photosystem I electron transport by high concentration of 3-(3–4,dichlorophenyl)-1,1-dimethyl urea in uncoupled chloroplasts. Plant Physiol 68: 1485–1487
Ramirez JM, DelCampa FF and Arnon DI (1968) Photosynthetic phosphorylation as energy source for protein synthesis and carbon dioxide assimilation by choroplasts. Proc Natl Acad Sci USA 59: 606–611
Reardon EM and Price CA (1983) Cytoplasmic regulation of chloroplast translation in Euglena gracilis. Arch Biochem Biophys 226(2): 433–440
Reith ME and Cattolico RA (1985) In vitro chloroplast protein synthesis by the chromophytic alga Olisthodiscus luteus. Biochemistry 24: 2550–2556
Sabater B and Rodriguez MT (1978) Control of chlorophyll degradation in detached leaves of barley and oat through kinetin on chlorophyllase levels. Physiol Plant 43: 274–276
Schmidt RJ, Richardson CB, Gillham NW and Boynton JE (1983) Site of synthesis of chloroplast ribosomal proteins in Chlamydomonas. J Cell Biol 96: 1451–1463
Shinozaki K, Ohme M, Tanaka M, Wakasugi T, Hayashida N, Matsubayashi T, Zaita N, Chungwongse J, Obokata J, Yamaguchi-Shinozaki K, Ohto C, Torazawa K, Meng BY, Sugita M, Deno H, Kamogashira T, Yamada K, Kusuda J, Takaiwa F, Kato A, Tohdoh N, Shimada H and Sugiura M (1986) The complete nucleotide sequence of the tobacco chloroplast genome: its gene organization and expression. EMBO J 5: 2043–2049
Siddell SG and Ellis RJ (1975) Protein synthesis in chloroplasts. VI. Characteristics and products of protein synthesis in etiolated and developing chloroplasts from pea leaves. Biochem J 146: 675–685
Silverthrone J and Ellis RJ (1980) Protein synthesis in chloroplasts. 8. Differential synthesis of chloroplast proteins during spinach Spinacia-oleracea leaf development. Biochim Biophys Acta 607(2): 319–330
Spencer D (1965) Protein synthesis by isolated spinach chloroplasts. Arch Biochem Biophys 111: 381–390
Spermulli LL (1982) Chloroplast elongation factor Tu evidence that it is the product of a chloroplast gene in Euglena gracilis. Arch Biochim Biophys 214: 734–741
Spreitzer RJ, Goldschmidt-Clermont M, Ralive M and Rochaix JD (1985) Nonsense mutations in Chlamydomonas chloroplasts gene for the large subunit of ribulose-1,5-bisophosphate carboxylase/oxygenase. Proc Natl Acad Sci USA 82: 5460–5464
Steinback KE, McIntosh L, Bogorad L and Arntzen CJ (1981) Identification of the triazine receptor protein as a chloroplast gene product. Proc Natl Acad Sci USA 78: 7463–7467
Subbaiah CC and Gnanam A (1987) In organello protein synthesis by isolated cashew chloroplasts. In: Mehta SL (ed.) Proc Nat Symp Photosyn and Nitrogen Metabolism (in press)
Subbaiah CC, Kulandaivelu G and Gnanam A (1987) Effect of ethidium bromide and UV-C radiation on chloroplast development. In: Rajamanickam C (ed.) Proc Second Internat Symp on Biomembranes, pp 347–358. New Delhi: Today and Tomorrow’s Publishers
Subbaiah CC, Mannan RM and Gnanam A (1987) Effect of polyamines on in organello protein synthesis by Sorghum chloroplasts. Biochem J (submitted)
Uma Bai V (1985) Development of chloroplast structure and function with reference to membrane proteins. Ph.D. Thesis, Madurai Kamaraj University
Uma Bai V, Daniell H and Gnanam A (1984) Role of photoelectron transport in the protein synthesis by isolated plastids. In: Sybesma C (ed.) Advances in Photosynthetic Research, Vol IV, pp 681–684. The Hague: Martinus-Nijhoff/Dr W Junk Publishers
Valliammai V (1985) Studies on Chloroplast Development of Chlorella protothecoides. Ph.D. Thesis, Madurai Kamaraj University
Vasconcelos AC (1976) Synthesis of proteins by isolated Euglena gracilis chloroplasts. Plant Physiol 58: 719–721
Waiden R and Leaver CJ (1981) Synthesis of chloroplast proteins during germination and early development of cucumber, Cucumis sativus cultivar long green ridge. Plant Physiol 67(6): 1090–1096
Watanabe A and Price CA (1982) Translation of mRNAs for subunits of chloroplast coupling factor I in spinach. Proc Natl Acad Sci USA 79: 6304–6308
Yamamoto T, Burke J, Autz G and Jagendorf AT (1981) Bound ribosomes of pea chloroplast thylakoid membranes: Location and release in vitro by high salt, puromycin and RNase. Plant Physiol 67: 940–949
Yu SM and Kao CH (1981) Retardation of leaf senescence by inhibitors of RNA and protein synthesis. Physiol Plant 52: 207–210
Zielinski RE and Price CA (1980) Synthesis of thylakoid membrane proteins by chloroplasts isolated from spinach Spinacia oleracea cytochrome B-559 and P700 chlorophyll a protein. J Cell Biol 85(2): 435–445
Zurawski G, Bohnert HJ, Whitfeld PR and Bottomley W (1982) Nucleotide sequence of the gene for the Mr 32 000 thylakoid membrane protein from Spinacea oleracea and Nicotiana debnevi predicts a totally conserved translational product of Mr 38 950. Proc Natl Acad Sci USA 79: 7699–7703
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Gnanam, A., Subbaiah, C.C., Mannan, R.M. (1988). Protein synthesis by isolated chloroplasts. In: Govindjee (eds) Molecular Biology of Photosynthesis. Springer, Dordrecht. https://doi.org/10.1007/978-94-009-2269-3_38
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DOI: https://doi.org/10.1007/978-94-009-2269-3_38
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