Abstract
In 1959 two Swedish researchers Jerker Porath and Per Gustaf Flodin reported their findings in Nature in a paper entitled “Gel Filtration: a method for desalting and group separation” (1). They described a method based on a column procedure which they claimed was “similar” to chromatography. The stationary phase they used was a cross linked dextran which within the year was launched by Pharmacia as a product called Sephadex®. Today, the paper is considered a classic in the methodology of biochemistry and Sephadex, after 29 years, is still used and referenced throughout the world (2).
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References
Gel filtration: A method for desalting and group separation. Nature 183 (1959) 1657–1659, Porath, J., Flodin, P.
On the history of the development of Sephadex. Chromatographia 23 (1987) 361–369, Janson, J-C.
Methodological aspects on gel filtration with special reference to desalting operations. J. Chromatogr. 5 (1961) 103–115, Flodin, P.
Separation of thyroglobulin, BSA,myoglobin and cytidine on Superose 6 prep grade at different flow rates. Chromatography 286 (1984) 295–306, Hagel, L., Anderson, T.J.
The gel filtration behaviour of proteins related to their molecular weights over a wide range. Biochemistry J. 96 (1965) 595–606, Andrews, P.
The large scale purification of insulin by gel filtration chromatography. Pharmacia LKB Biotechnology AB, Uppsala, Sweden.
Scale up to process scale: A systematic guide to the design and scale up of protein purification for the biotechnology industry. Pharmacia LKB Biotechnology AB, Uppsala, Sweden.
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© 1989 Kluwer Academic Publishers
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Moore, P.M. (1989). Gel Filtration Chromatography. In: Rodrigues, A.E., LeVan, M.D., Tondeur, D. (eds) Adsorption: Science and Technology. NATO ASI Series, vol 158. Springer, Dordrecht. https://doi.org/10.1007/978-94-009-2263-1_29
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DOI: https://doi.org/10.1007/978-94-009-2263-1_29
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