Skip to main content
SpringerLink
Log in

Influence of Growth Conditions on Production and Composition of PHA by Pseudomonas Oleovorans

  • Chapter
Novel Biodegradable Microbial Polymers

Part of the book series: NATO ASI Series ((NSSE,volume 186))

Abstract

Poly-3-hydroxyalkanoates are biological polymers with thermoplastic properties. PHA generally accumulates under unbalanced growth conditions when an excess of carbon is available but growth is limited by some essential nutrient (1). Pseudomonas oleovorans accumulates PHA under ammonium, sulfate, magnesium and phosphate limitations (2). Our study shows that oxygen limitation also stimulates PHA production. Synthesis and accumulation of the polymer are under strict environmental control as is intracellular degradation. PHA degradation starts when the carbon source falls below a critical level. We describe a method to exactly determine the beginning of intracellular PHA degradation during a fermentation process.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 169.00
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 219.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 219.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Reference

  1. Dawes, E.A., and P.J. Senior, 1973, Adv. Microb. Physiol. 10: 135–266

    Article  CAS  Google Scholar 

  2. Lageveen, R.,1986, Ph.D. Thesis, University of Groningen, The Netherlands

    Google Scholar 

  3. Brandt, H., R.A. Gross, R.W. Lenz, R.C. Fuller, 1988, Appl. Environ. Microbiol. 54: 1977–1982

    Google Scholar 

  4. Haywood, G.W., A.J. Anderson, and E.A. Dawes, 1989, Biotechnol. Letters 11: 471–476

    Article  CAS  Google Scholar 

  5. Huisman, G.W., O. DeLeeuw, G. Eggink and B. Witholt,1989, Appl. Environ. Microbiol. 55: 1949–1954

    Google Scholar 

  6. Lageveen, R.G., et al., 1988, Appl. Environ. Microbiol. 54: 2924–2932

    CAS  Google Scholar 

  7. Bluhm, T.L., et al.,1986, Macromolecules 19: 2871–2876

    Article  CAS  Google Scholar 

  8. Wallen, L.L., and W.K. Rohwedder, 1974, Environ. Sci. Technol. 8: 576–579

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Dept.of Biochemistry, University of Massachusetts, Amherst, MA, 01003, USA

    E. J. Knee Jr., M. Wolf & R. C. Fuller

  2. Polymer Science and Engineering, Amherst, MA, 01003, USA

    R. W. Lenz

Authors
  1. E. J. Knee Jr.
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. M. Wolf
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. R. W. Lenz
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. R. C. Fuller
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

Editors and Affiliations

  1. Department of Applied Biology, University of Hull, Hull, UK

    Edwin A. Dawes

Rights and permissions

Reprints and permissions

Copyright information

© 1990 Kluwer Academic Publishers

About this chapter

Cite this chapter

Knee, E.J., Wolf, M., Lenz, R.W., Fuller, R.C. (1990). Influence of Growth Conditions on Production and Composition of PHA by Pseudomonas Oleovorans . In: Dawes, E.A. (eds) Novel Biodegradable Microbial Polymers. NATO ASI Series, vol 186. Springer, Dordrecht. https://doi.org/10.1007/978-94-009-2129-0_39

Download citation

  • DOI: https://doi.org/10.1007/978-94-009-2129-0_39

  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-94-010-7458-2

  • Online ISBN: 978-94-009-2129-0

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics

Search

Navigation