Abstract
PHA concentration was determined in 35 Spanish wetlands including deep, oligotrophic lakes, hypersaline ponds, coastal lagoons, high mountain lakes, karstic lakes and a variety of shallow ponds. PHA was found in all of them in concentrations between 0.05 and 5.0 mg/l. The PHA concentration was strongly correlated with total bacterial numbers. PHA was followed through a year cycle in Lake Cisó. Phototrophic bacteria reach large abundances in this lake and PHA concentration changed seasonally according to their biomass. PHA specific content was high during mixing and very low during stratification. Essentially no PHA reached the sediments through sedimentation, indicating that it was used by the phototrophic bacteria or decomposed by heterotrophic bacteria in the water column. PHA in planktonic systems is the consequence of several physiological and environmental factors acting together and its interpretation is, therefore, not straightforward.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Brandl, H., Gross, R. A., Lenz, R. W. and Fuller, R. C. (1988) ‘Pseudomonas oleovorans as a source of poly(ß-hydroxyalkanoates) for potential applications as biodegradable polyesters’, Appl. Environ. Microbiol. 54, 1977–1982.
Balkwill, D. L., Leach, F. R., Wilson, J. T., McNabb, J. F. and White, D. C. (1988) ‘Equivalence of microbial biomass measures based on membrane lipid and cell wall components, adenosine triphosphate, and direct counts in subsurface aquifer sediments’, Microb. Ecol. 16, 73–84.
Byrom, D. (1987) ‘Polymer synthesis by microorganisms: technology and economics’, Trends in Biotech. 5, 242–250.
Dawes, E. A. and Senior, P. J. (1973) ‘The role and regulation of energy reserve polymers in micro-organisms’, Adv. Microb. Physiol. 10, 135–266.
Esteve, I., Montesinos, E., Mitchell, J. G. and Guerrero, R. (1990) ‘A quantitative ultrastructural study ofChromatium minus in the bacterial layer of Lake Cisó ( Spain)’, Arch. Microbiol. 153, 316–323.
Findlay, R. H. and White, D. C. (1983) ‘Polymeric beta-hydroxyalkanoates from envi- ronmental samples and Bacillus megaterium’, Appl. Environ. Microbiol. 45, 71–78
Guerrero, R., Montesinos, E., Pedrós-Alió, C., Esteve, I., Mas, J., Van gemerden, H., Hofman, P. A. G. and Bakker, J. F. (1985) ‘Phototrophic sulfur bacteria in two Spanish lakes: Vertical distribution and limiting factors’, Limnol. Oceanogr. 30, 919–931.
Heinzle, E. and Lafferty, R. M. (1980) ‘A kinetic model for growth and synthesis of poly-ß-hydroxybutyric acid (PHB) in Alcaligenes eutrophus H 16’, European J. Appl. Microbiol. Biotechnol. 11, 8–16.
Herron, J. S., King, J. D. and White, D. C. (1978) ‘Recovery of poly-ß-hydroxybutyrate from estuarine microflora’, Appl. Environ. Microbiol. 35, 251–257.
Holmes, P. (1985) ‘Applications of PHB - A microbially produced biodegradable thermoplastic’, Phys. Technol. 16, 32–36.
Ingvorsen, K. and Jorgensen, B. B. (1979) ‘Combined measurement of oxygen and sulfide in water samples’, Limnol. Oceanogr. 24, 390–393.
Lageveen, R. G., Huisman, G. W., Preusting, H., Ketelaar, P., Eggink, G. and Witholt, B. (1988) ‘Formation of polyesters by Pseudomonas oleovorans: effect of substrates on formation and composition of poly-(R)-3-hydroxyalkanoates and poly(R)-3-hydroxyalkenoates’, Appl. Environ. Microbiol. 54, 2924–2932.
Law, J. H. and Slepecky, R. A. (1961) ‘Assay of poly-ß-hydroxybutyric acid’, J. Bacteriol. 82, 33–36.
Merrick, J. M. (1978) ‘Metabolism of reserve materials’, in R.K. Clayton and W.R. Sistrom (eds.), The Photosynthetic Bacteria, pp. 199–219. Plenum. New York
Nickels, J. S., King, J. D. and White, D. C. (1979) ‘Poly-ß-hydroxybutyrate accumulation as a measure of unbalanced growth of the estuarine detrital microbiotâ, Appl. Environ. Microbiol. 37, 459–465.
Pedrós-Alió, C. (1989) ‘Toward an autecology of bacterioplankton’ in U. Sommer (ed.), Plankton Ecology. Succession in Plankton Communities, Springer/Verlag, Berlin.
Pedrós-Alió, C., Mas, J. and Guerrero, R. (1985) ‘The influence of poly-ß-hydroxybutyrate accumulation on cell volume and buoyant density in Alcaligenes eutrophus’, Arch. Microbiol. 143, 178–184.
Pedrós-Alió, C., Gasol, J. M. and Guerrero, R. (1986) ‘Microbial ecology of sulfurous Lake Cisó’, in F. Megusar and M. Gantar (eds.), Perspectives in Microbial Ecology, Slovene Soc. Microbial., Ljubljana, Yugoslavia, 638–644.
Shively, J. M., Bryant, D. A., Fuller, R. C., Konopka, A. E., Stevens jr., S. E. and Strohl, W. R. (1989) ‘Functional inclusion bodies in prokaryotic cells’, Int. Rev. Cytol. 113, 35–100.
Suzuki, T., Yamane, T. and Shimizu, S. (1986) ‘Mass production of poly-ß-hydroxybutyric acid by fully automatic fed-batch culture of methylotroph’, Appl. Microbiol. Biotechnol. 23, 322–329.
Takahashi, M. and Ichimura, S. (1968) ‘Vertical distribution and organic matter production of phototrophic sulfur bacteria in Japanese lakes’, Limnol. Oceanogr. 13, 644–655.
Van Gemerden, H., Montesinos, E., Mas, J. and Guerrero, R. (1985) ‘Diel cycle of metabolism of phototrophic purple sulfur bacteria in Lake Cisó ( Spain)’, Limnol. Oceanogr. 30, 932–943.
Vestal, J. R. and White, D. C. (1989) ‘Lipid analysis in microbial ecology. Quantitative approaches to the study of microbial communities’, BioScience 39, 535–541.
White, D. C. (1986) ‘Quantitative physicochemical characterization of bacterial habitats’, in J. S. Poindexter and E. R. Leadbetter (eds.), Bacteria in Nature. Vol. 2, Plenum, New York.
Zimmermann, R. and Meyer-Reil, L.-A. (1974) ‘A new method for fluorescence stain- ing of bacterial populations on membrane filters’, Kiel. Meeresforsch. 30, 24–27.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1990 Kluwer Academic Publishers
About this chapter
Cite this chapter
Pedrós-Alió, C., Mas-Castellà, J., Mas, J., Guerrero, R. (1990). Polyhydroxyalkanoate Accumulation in Planktonic and Anaerobic Environments. In: Dawes, E.A. (eds) Novel Biodegradable Microbial Polymers. NATO ASI Series, vol 186. Springer, Dordrecht. https://doi.org/10.1007/978-94-009-2129-0_22
Download citation
DOI: https://doi.org/10.1007/978-94-009-2129-0_22
Publisher Name: Springer, Dordrecht
Print ISBN: 978-94-010-7458-2
Online ISBN: 978-94-009-2129-0
eBook Packages: Springer Book Archive