Abstract
The poly-ß-hydroxybutyrate biosynthetic pathway of Alcaligenes eutrophus H16 has been cloned into Escherichia coli, where it has been the subject of a series of molecular analyses. The DNA sequence of the pathway has been determined and analyzed for open reading frames and secondary structure. In vivo transcription/translation studies have identified the size of the gene products and these have been aligned with specific open reading frames. A putative promoter has been located approximately 300 bases upstream from the start of translation on the basis of deletion mutants and consensus sequence comparisons. PHB is made to levels approaching 90% of the cell weight, in large part due to the gene dosage effect of the multicopy plasmid on which the pathway resides. PHB production varies considerably in response to media alterations.
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© 1990 Kluwer Academic Publishers
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Janes, B., Hollar, J., Dennis, D. (1990). Molecular Characterization of the Poly-ß-Hydroxybutyrate Biosynthetic Pathway of Alcaligenes Eutrophus H16. In: Dawes, E.A. (eds) Novel Biodegradable Microbial Polymers. NATO ASI Series, vol 186. Springer, Dordrecht. https://doi.org/10.1007/978-94-009-2129-0_15
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DOI: https://doi.org/10.1007/978-94-009-2129-0_15
Publisher Name: Springer, Dordrecht
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