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The Use of the Polymerase Chain Reaction to Detect Specific Sequences in Transformed Plant Tissues

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Part of the book series: Current Plant Science and Biotechnology in Agriculture ((PSBA,volume 9))

Abstract

The polymerase chain reaction is a very powerful technique which is now used in many areas of biology. Two recent bgoks discussing the merits and practicalities of PCR are recommended1,2. A simple and obvious use of PCR in plant transformation studies is to use it to confirm the presence and/or the expression of a foreign gene in transgenic plant tissues and the work reported here was intended to examine a number of parameters affecting the successful amplification of foreign genes from plant tissues. A recent report byLassner et al.3 also shows the potential of PCR for analysis of foreign genes in plant tissues.

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References

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© 1990 Kluwer Academic Publishers

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Hamill, J.D., Rounsley, S., Spencer, A., Todd, G., Rhodes, M.J.C. (1990). The Use of the Polymerase Chain Reaction to Detect Specific Sequences in Transformed Plant Tissues. In: Nijkamp, H.J.J., Van Der Plas, L.H.W., Van Aartrijk, J. (eds) Progress in Plant Cellular and Molecular Biology. Current Plant Science and Biotechnology in Agriculture, vol 9. Springer, Dordrecht. https://doi.org/10.1007/978-94-009-2103-0_27

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  • DOI: https://doi.org/10.1007/978-94-009-2103-0_27

  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-94-010-7445-2

  • Online ISBN: 978-94-009-2103-0

  • eBook Packages: Springer Book Archive

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