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Micropropagation under photoautotrophic conditions

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Micropropagation

Abstract

Micropropagation has many advantages over conventional vegetative propagation and its commercial use in horticulture, agriculture and forestry is currently expanding worldwide. However, its widespread commercial use for major crops is still restricted as a result of its relatively high production costs. The high production costs in conventional micropropagation are mainly due to high labor costs, limited rates of growth during multiplication, poor rooting, and low survival rates of the plantlets during acclimatization.

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References

  • Aitken-Christie J (1988) Development of a semi-automated micropropagation system, Acta Hort 230: 81–86.

    Google Scholar 

  • Ando T (1978) Gaseous environment in the airtight culture vessel containing orchids, Abstr Annual Autumn Meet Jap Soc Hort Sci: 368–369.

    Google Scholar 

  • Aoki S, Oda M (1988) Sensing of photosynthetic capacities of seedling of lettuce with chlorophyll fluorescence, Acta Hort 230: 363–370.

    Google Scholar 

  • Arai S, Asao H, Kawabata R, Kobatake H (1989) Effects of CO2 enrichment on the growth of strawberry plantlets regenerated from shoot tip culture, J Jap Soc Hort Sci 58 (special issue 1): 252–253.

    Google Scholar 

  • Bastiaens L (1983) Endogenous bacteria in plants and their implications in tissue culture — A review, Med Fac Landbouww Rijksuniv Gent 48: 1–11.

    Google Scholar 

  • Brainerd KE, Fuchigami LJ (1981) Acclimatization of aseptically cultured apple plants to low relative humidity, J Amer Soc Hort Sci 106: 515–518.

    Google Scholar 

  • Brown DCW, Leung DWM, Thorpe TA (1979) Osmotic requirement for shoot formation in tobacco callus, Physiol Plant 46: 36–41.

    Article  CAS  Google Scholar 

  • Capellades MQ (1989) Histological and ecophysiological study of the changes occurring during the acclimatization of in vitro cultures (pp 1–98) PhD-thesis State University Gent, Belgium.

    Google Scholar 

  • Chong C, Pua EC (1985) Carbon nutrition of Otawa 3 apple rootstock during stages of in vitro propagation, J Hort Sci 60: 285–290.

    CAS  Google Scholar 

  • Debergh P (1983) Effects of agar brand and concentration on the tissue culture medium, Physiol Plant 59: 270–276.

    Article  CAS  Google Scholar 

  • Debergh P (1987) Improving micropropagation, Intl Assoc Plant Tissue Culture, Newsletter 51: 2–10.

    Google Scholar 

  • Debergh P (1988) Improving mass propagation of in vitro plantlets. In: The organizing committee Intl Symp High Technology in Protected Cultivation (Ed) Special lectures: Horticulture in high technology era (45–57) Tokyo.

    Google Scholar 

  • De Bry L (1988) Prospects in the mating of robotics to plant tissue culture and research. In: The organizing committee Intl Symp High Technology in Protected cultivation (Ed) Special lectures: Horticulture in high technology era (61–72) Tokyo.

    Google Scholar 

  • De Proft MP, Maene ML, Debergh P (1985) Carbon dioxide and ethylene evolution in the culture atmosphere of Magnolia cultured in vitro, Physiol Plant 65: 375–379.

    Article  Google Scholar 

  • Desjardins Y, Gosselin A, Yelle S (1987) Acclimatization of in vitro strawberry plantlets under CO2 enriched environment and supplemental lighting, J Amer Soc Hort Sci 112: 846–851.

    Google Scholar 

  • Desjardins Y, Laforge F, Lussier C, Gosselin A (1988) Effect of CO2 enrichment and high photosynthetic photon flux on the development of autotrophy and growth of tissue-cultured strawberry, raspberry and asparagus plants, Acta Hort 230: 47–57.

    Google Scholar 

  • Doi M, Noguchi M, Asahira T (1986) Effects of CO2 enrichment on the in vitro culture of Phalaenopsis, Abstr Ann Meet Environ Control Biology (Japan): 64–65.

    Google Scholar 

  • Donnelly DJ, Vidaver WE (1984) Pigment content and gas exchange of red raspberry in vitro and ex vitro, J Amer Soc Hort Sci 109: 177–181.

    CAS  Google Scholar 

  • Driver JA, Suttle GRL (1985) Nursery handling of propagules. In: Bonga JM, Durzan DJ (Eds) Ceil and tissue culture in forestry (pp 320–335) Martinus Nijhoff Pubis, Dordrecht, Boston, Lancaster.

    Google Scholar 

  • Fujita N (1989) Application of robotics for a masspropagation system, Abstr 40th Annu Meet Tissue Culture Assoc (USA) Orlando, Florida.

    Google Scholar 

  • Fujiwara K, Kozai T, Watanabe I (1987) Measurements of carbon dioxide gas concentration in stoppered vessels containing tissue cultured plantlets and estimates of net photosynthetic rates of the plantlets, J Agrie Meteorol Japan 43: 21–30.

    Article  Google Scholar 

  • Fujiwara K, Kozai T, Watanabe I (1988) Development of a photoautotrophic tissue culture system for shoots and/or plantlets at rooting and acclimatization stages, Acta Hort 230: 153–158.

    Google Scholar 

  • Fujiwara K, Kozai T, Nakajyo Y, Watanabe I (1989) Effects of closures and vessels on light intensities in plant tissue culture vessels, J Agrie Meterol Japan 45: 143–149.

    Article  Google Scholar 

  • George EF, Sherrington PD (1984) Plant propagation by tissue culture (pp 1–709) Exegetics Ltd., England.

    Google Scholar 

  • Grout BWW, Ashton MJ (1978) Transplanting of cauliflower plants regenerated from meristem culture II. Carbon dioxide fixation and the development of photosynthetic ability, Hort Res 17: 65–71.

    CAS  Google Scholar 

  • Hayashi M, Kozai T (1987) Development of a facility for accelerating the acclimatization of tissue-cultured plantlets and the test cultivations. In: Ducaté G, Jacob M, Simpson A (Eds) Plant micropropagation in horticultural industries (pp 123–134) Arlon, Belgium.

    Google Scholar 

  • Hayashi M, Nakayama M, Kozai T (1988) An application of the acclimatization unit for growth of carnation expiants and for rooting and acclimatization of the plantlets, Acta Hort 230: 189–194.

    Google Scholar 

  • Hempel M, Meszka K (1988) Modelling of micropropagation, Acta Hort 230: 137–144.

    Google Scholar 

  • Horn ME, Dalton CC (1984) Photosynthetic cell cultures and their biotechnological applications, Intl Assoc Plant Tissue Culture, Newsletter 43: 2–7.

    Google Scholar 

  • Horn ME, Widholm JM (1984) Aspects of photosynthetic cell cultures. In: Collins GB, Petolia JG (Eds) Applications of genetic engineering to crop improvement (pp 113–161) Martinus Nijhoff/Dr. W.Junk Pubis, Dordrecht.

    Chapter  Google Scholar 

  • Hoshi T, Kozai T (1984) Knowledge-based and hierarchically distributed online control system for greenhouse management, Acta Hort 148: 301–308.

    Google Scholar 

  • Kaneko T, Kato R, Takatsuji M (1988) Effects of carbon source on adventitious root formation of butter-head type lettuce, Abstr Ann Meet Environ Control Biol, Japan.

    Google Scholar 

  • Kimball SL, Beversdorf WD, Bingham ET (1975) Influence of osmotic potential on the growth and development of soybean tissue cultures, Crop Sci 15: 750–752.

    Article  CAS  Google Scholar 

  • Kozai T (1988a) High technology in protected cultivation. In: Organizing Committee Intl Symp High Technology in Protected Cultivation (Ed) Special Lectures: High technology in protected cultivation (pp 1–43), Tokyo.

    Google Scholar 

  • Kozai T (1988b) Autotrophic (sugar-free) tissue culture for promoting the growth of plantlets in vitro and for reducing biological contamination, Intl Symp Application of Biotechnology for Small Industries (pp 1–21) Bangkok, Thailand.

    Google Scholar 

  • Kozai T (1990) Autotrophic micropropagation. In: Bajaj YPS (Ed) Biotechnology in agriculture and forestry (in press) Springer Verlag, Berlin.

    Google Scholar 

  • Kozai T, Iwanami Y (1988) Effects of CO2 enrichment and sucrose concentration under high photon fluxes on plant growth of carnation (Dianthus caryophyllus L.) in tissue culture during the preparation stage, J Japan Soc Hort Sci 57: 279–288.

    Article  Google Scholar 

  • Kozai T, Sekimoto K (1988) Effects of the number of air changes per hour of the stoppered vessel and the photosynthetic photon flux on the carbon dioxide concentration inside the vessel and the growth of strawberry plantlets in vitro, Environment Control Biol 26: 21–29.

    Article  CAS  Google Scholar 

  • Kozai T, Fujiwara K, Watanabe I (1986a) Relation between the culture medium composition and water potential of liquid culture media, J Agric Meteorol 42: 1–6.

    Article  Google Scholar 

  • Kozai T, Fujiwara K, Watanabe I (1986b) Effects of stoppers and vessels on gas exchange rates between inside and outside of vessels closed with stoppers, J Agric Meteorol 42: 119–127.

    Article  Google Scholar 

  • Kozai T, Hayashi M, Hirosawa Y, Kodama T, Watanabe I (1987a) Development of the acclimatization unit for accelerating the plantlet growth and the test cultivation, J Agric Meteorol 42: 349–358.

    Article  Google Scholar 

  • Kozai T, Iwanami Y, Fujiwara K (1987b) Effects of CO2 enrichment on the plantlet growth during the multiplication stage, Plant Tissue Culture Letters 4: 22–26.

    Article  Google Scholar 

  • Kozai T, Koyama Y, Watanabe I (1987c) Energy balance of a plant growth lamp with a water cooled reflector for reducing cooling load, Abstr Annu Meet Agric Meteor Japan: 186–187.

    Google Scholar 

  • Kozai T, Oki H, Fujiwara K (1987d) Environmental control for masspropagation of tissue-cultured plantlets, Abstr Annu Spring Meet Japan Soc Hort Sci: 366–377.

    Google Scholar 

  • Kozai T, Oki H, Fujiwara K (1987e) Effects of CO2 enrichment and sucrose concentration under high photosynthetic photon fluxes on growth of tissue-cultured Cymbidium plantlets during the preparation stage. In: Ducaté G, Jacobs M, Simpson A (Eds) Plant micropropagation in horticultural industries (pp 135–141) Arlon, Belgium.

    Google Scholar 

  • Kozai T, Iwabuchi K, Watanabe I (1988a) Effects of low dark period temperatures and CO2 enrichment during the photoperiod on the growth of strawberry plantlets in vitro (1) and (2), Abstr Annu Autumn Meet Jap Soc Hort Sci: 270–271 and 272–273.

    Google Scholar 

  • Kozai T, Koyama Y, Watanabe I (1988b) Multiplication of potato plantlets in vitro with sugar free medium under high photosynthetic photon flux, Acta Hort 230: 121–127.

    Google Scholar 

  • Kozai T, Kubota C, Watanabe I (1988c) Effects of basal medium composition on the growth of carnation plantlets in auto- and mixotrophic tissue culture, Acta Hort 230: 159–166.

    Google Scholar 

  • Kozai T, Watanabe K, Kinoshita I (1988d) Effects of sugar composition and the degrees of mixotrophy on the water potential of the medium and the growth of strawberry plantlets in vitro, Abstr Annu Autumn Meet Jap Soc Hort Sci: 270–271.

    Google Scholar 

  • Kozai T, Iwabuchi K, Watanabe K, Watanabe I (1989a) Effects of CO2 enrichment and sugar-free medium on the growth and nutrient uptake of strawberry plantlets in vitro, J Jap Soc Hort Sci 58 (Special issue 1): 254–255.

    Google Scholar 

  • Kozai T, Kubota C, Nakayama M (1989b) Net photosynthetic rates of plantlets in vitro under natural and forced ventilation conditions, J Japan Soc Hort Sci 58 (Special issue 1): 250–251.

    Google Scholar 

  • Kozai T, Nishiuchi N, Fujiwara K, Watanabe I (1989c) Effects of planting density in the vessel on the growth and net photosynthetic rates of potato plantlets in photoautotrophic micropropagation, J Japan Soc Hort Sci 58 (Special issue 1): 244–245.

    Google Scholar 

  • Kozai T, Ohde N, Kubota C (1989d) Effects of in vitro environment on the growth of plantlets/seedlings of Chinese mustard, J Japan Soc Hort Sci 58 (Special issue 1): 236–237.

    Google Scholar 

  • Kozai T, Takazawa A, Fujiwara K, Watanabe I (1989e) Effects of CO2 enrichment, PPF and sucrose concentration in the medium on the growth of tobacco seedlings in vitro, J Japan Soc Hort Sci 58 (Special issue 1): 240–241.

    Google Scholar 

  • Kozai T, Watanabe K, Watanabe I (1989f) Relationship between dry matter increase of strawberry plantlets in vitro and nutrient uptake, J Japan Soc Hort Sci 58 (Special issue 1): 248–249.

    Google Scholar 

  • Larcher W (1980) (Ed) Ecological plant physiology. Springer-Verlag, 94–102.

    Google Scholar 

  • Miles GE (1988) Robotic Transplanting. In: Organizing Committee Intl Symp High Technology in Protected Cultivation (Ed) Special lectures: Horticulture in high technology era (pp 75–86) Tokyo.

    Google Scholar 

  • Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures, Physiol Plant 15: 473–497.

    Article  CAS  Google Scholar 

  • Nakayama M, Kozai T, Watanabe I (1989) Effects of sucrose concentration in the medium and ventilation methods on the net photosynthetic rates of potato explants in vitro, Abstr Biannual Meet Jap Plant Tissue Culture Assoc.

    Google Scholar 

  • Pierik RLM (1988) Handicaps for the large scale commercial application of micropropagation, Acta Hort 230: 63–71.

    Google Scholar 

  • Pospisilova J, Catsky J, Solarova J, Ticha I (1987) Photosynthesis of plant regenerants, Specificity of in vitro conditions and plant response, Biol Plant (Praha) 29: 415–421.

    CAS  Google Scholar 

  • Pospisilova J, Solarova J, Catsky J, Ondrej M (1988) The photosynthetic characteristics during the micropropagation of tobacco and potato plants, Photosynthetica 22: 205–213.

    CAS  Google Scholar 

  • Shimada N, Tanaka F, Kozai T (1988) Effects of low O2 concentration on net photosynthesis of C3 plantlets in vitro, Acta Hort 230: 171–175.

    Google Scholar 

  • Tanaka M, Jinno K, Goi M, Higashiura T (1988) The use of disposable fluorocarbon polymer film culture vessel in micropropagation, Acta Hort 230: 73–80.

    Google Scholar 

  • Walker PN, Heuser CW, Heinemann PH (1988) Micropropagation: Studies of gaseous environments, Acta Hort 230: 145–152.

    Google Scholar 

  • Wardle K, Dobbs EB, Short KC (1983) In vitro acclimatization of aseptically cultured plantlets to humidity, J Amer Soc Hort Sci 108: 386–389.

    Google Scholar 

  • Weathers P, Cheetham RD, Giles KL (1988) Dramatic increases in shoot number and length for Musa, Cordyline and Nephrolepis using nutrient mists, Acta Hort 230: 39–44.

    Google Scholar 

  • Zelitch I (1971) Photosynthesis, photorespiration and plant productivity, Academic Press, pp 347.

    Google Scholar 

  • Zelitch I (1975) Improving the efficiency of photosynthesis, Science 188: 626–633.

    Article  PubMed  CAS  Google Scholar 

  • Ziv M, Meir G, Halevy AH (1983) Factors affecting the production of hardened glaucous carnation plantlets in vitro, Plant Cell Tissue Organ Culture 2: 55–65.

    Article  Google Scholar 

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Authors
  1. T. Kozai
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Editors and Affiliations

  1. Department of Horticulture, State University of Gent, Belgium

    P. C. Debergh

  2. United State Department of Agriculture (ARS), Beltsville, Maryland, USA

    R. H. Zimmerman

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© 1991 Kluwer Academic Publishers

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Kozai, T. (1991). Micropropagation under photoautotrophic conditions. In: Debergh, P.C., Zimmerman, R.H. (eds) Micropropagation. Springer, Dordrecht. https://doi.org/10.1007/978-94-009-2075-0_26

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  • DOI: https://doi.org/10.1007/978-94-009-2075-0_26

  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-0-7923-0819-5

  • Online ISBN: 978-94-009-2075-0

  • eBook Packages: Springer Book Archive

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