Abstract
Intestinal epithelial tight junctions are the rate limiting barriers to passive transepithelial movement of hydrophilic solutes. We have studied these important barriers in Ussing chambers using isolated sheets of mammalian intestinal epithelia and monolayers of the T84 human intestinal epithelial cell line. Studies have included both electrophysiologic and morphologic techniques. These studies show tight junctions to be highly dynamic structures which may be influenced pharmacologically or by physiologic challenges. Additionally, elements of inflammatory responses, such as the presence of the cytokine gamma-interferon, or the transepithelial migration by polymorphonuclear leukocytes, lead to deterioration in TJ barrier function. Such events may contribute to the epithelial barrier defects seen in patients with IBD.
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© 1990 Kluwer Academic Publishers
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Madara, J.L., Nash, S., Moore, R., Shapiro, M., Carlson, S., Delp, C. (1990). Barrier Function of Intestinal Epithelial Tight Junctions (TJ). In: Rachmilewitz, D., Zimmerman, J. (eds) Inflammatory Bowel Diseases 1990. Developments in Gastroenterology, vol 11. Springer, Dordrecht. https://doi.org/10.1007/978-94-009-1980-8_4
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DOI: https://doi.org/10.1007/978-94-009-1980-8_4
Publisher Name: Springer, Dordrecht
Print ISBN: 978-94-010-7385-1
Online ISBN: 978-94-009-1980-8
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