Abstract
The oxidation of methanol by yeasts has been reported by numerous authors and excellent reviews of the biochemistry of methanol assimilation have been written (Veenhuis et al. 1983; Large & Bamforth 1988). The first step in assimilation of methanol by methylotrophic yeasts is its oxidation to formaldehyde with concomitant production of hydrogen peroxide.
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References
Bauer R, 1985. Miles Laboratories Inc. EEC Patent number 0164008.
Belghith H, Romette JL and Thomas D, 1987. An enzyme electrode for online determination of ethanol and methanol. Biotechnology and Bioengineering 30: 1001–1005.
Bellion E and Goodman JM, 1987. Proton ionophores prevent assembly of a peroxisomal protein. Cell 48: 165–173.
Berglin EH, Edland M-BK, Nyberg GK and Carlsson J, 1982. Potentiation by L-cysteine of the bacteriocidal effect of hydrogen peroxide in E. coli. Journal of Bacteriology 152: 81–88.
Brooke AG, Dijkhuizen L and Harder W, 1986. Regulation of flavin biosynthesis in the methylotrophic yeast H. polymorpha. Archives in Microbiology 145: 62–70.
Bruinenberg PG, Veenhuis M, van Dijken JP, Duine JA and Harder W, 1982. A quantitative analysis of selective inactivation of peroxisomal enzymes in the yeast H. polymorpha by high performance liquid chromatography. FEMS Letters 15: 45–50.
Bystrykh LV, Romanov VP, J Steczko and Trotsenko YA, 1989. Catalytic variability of alcohol oxidase from the methylotrophic yeast Hansenula polymorpha. Biotechnology and Applied Biochemistry 11: 184–192.
Clarke LC Jr and Lyons C, 1962. Electrode systems for continuous monitoring in cardiovascular surgery. Annals of the New York Academy of Science 105: 29–45.
Clarke LC Jr, 1987. The enzyme electrode in Biosensors, Fundamental and Applications Ed. Turner APF, Karube I and Wilson G.
Couderc CL and Baratti J, 1980. Oxidation of methanol by the yeast Pichia pastoris. Purification and properties of the alcohol oxidase. Agricultural and Biological Chemistry 44: 2279–2289.
Cox RB, Steer DC and Woodward JR, 1982. Unilever UK plc. UK patent number GB 2101 167. Distel B, Veenhuis M and Tabak HF, 1987. Import of alcohol oxidase into peroxisomes of Saccharomyces cerevisiae. The EMBO Journal 6: 3111–3116.
Eggeling L, Paschke M and Sahm H, 1981. Process for the microbial production of alcohol oxidase. United States Patent number 4 250 261.
Eggeling L and Sahm H, 1980. Regulation of alcohol oxidase synthesis in Hansenula polymorpha: over synthesis during growth on mixed substrates and induction by methanol. Archives of Microbiology 127: 119–124.
Fuji T and Tonomura K, 1972. Oxidation of methanol, formaldehyde and formate by Candida sp. Agricultural and Biological Chemistry 36: 2297–2306.
Geissler J and Hemmerich P, 1981. Yeast methanol oxidases: an unusual type of flavoprotein. FEBS Letters 126: 152–156.
Gibson TD and Woodward, 1986. Automated determination of ethanol using the enzyme alcohol oxidase. Analytical Proceedings 23: 360–362.
Gibson TD and Woodward JR, 1988. Continuous, reliable on-line analysis of fermentation media by simple enzymic/spectrophotometric assays. Analytica Chimica Acta 213: 61–68.
Goodman JM, Scott CW, Donahue N and Atherton JP, 1984. Alcohol oxidase assembles posttranslationally into the peroxisome of Candida boidinii. Journal of Biological Chemistry 259: 8485–8493.
Goodman JM, Maher J, Silver PA, Pacifico A and Sanders D, 1986. The membrane proteins of the methanol-induced peroxisome of Candida boidinii. Journal of Biological Chemistry 261: 3464–3468.
Giuseppin MLF, 1988. PhD Thesis. Optimisation of methanol oxidase production by Hansenula polymorpha. University of Delft.
Giuseppin MLF, van Eijk HMJ, Verduyn C, Bante I and van Dijken JP, 1988a. Production of catalase-free alcohol oxidase by Hansenula polymorpha. Applied Microbiology and Biotechnology 28: 14–19.
Giuseppin MLF, van Eijk HMJ, Bos A, Verduyn C and van Dijken JP, 1988b. Utilisation of methanol by a catalase-negative mutant of Hansenula polymorpha. Applied Microbiology and Biotechnology 28: 286–292.
Giuseppin MLF, van Eijk HMJ and Bes BCM, 1988c. Molecular regulation of alcohol oxidase formation in continuous cultures of Hansenula polymorpha. Biotechnology and Bioengineering 32: 577–583.
Herzenberg GR and Rogerson M, 1985. Use of alcohol oxidase to measure the methanol produced during the hydrolysis of D- and L-methyl-3-hydroxy butyric acid. Analytical Biochemistry 149: 354–357.
Holzer H, 1976. Catabolite inactivtion in yeasts. Trends in Biochemical Sciences 1: 178–181.
Hopkins TR, 1980. A method for producing alcohol oxidase. European Patent Application No. 0019937.
Hopkins TR, 1988. Stabilised alcohol oxidase compositions and method for producing same. United States Patent number 4.729956.
Hopkins TR and Muller F, 1987. Biochemistry of alcohol oxidases in Proceedings of the 5th International Symposium on Microbial Growth on C1 Compounds. Eds van Verseveld HW, Duine JA. Martinus Nijhof Publishers, Dordrecht. 150–157.
Kato N, Omori Y, Tani Y and Ogata K, 1976. Alcohol oxidases of Kloeckera sp. and Hansenula polymorpha. European Journal of Biochemistry 64: 341–350.
Klavens JA and Bennett RD, 1986. Determination of methanol using alcohol oxidase and its application to methyl ester content of pectins. Journal of Agricultural and Food Chemistry 34: 597–599.
Large PJ and Bamforth CW, 1988. Basic physiology and biochemistry of methylotrophic yeasts in Methylotrophy and Biotechnology. Longman Scientific and Technical 105–122.
Majkic-Singh N and Berkes I, 1980. Spectrophotometric determination of ethanol by an enzymic method with 2.2’-azino-bis(3-ethylbenzothiazoline-6-sulfonate). Analytica Chimica Acta 115: 401–405.
Kato N, Tani Y and Yamada H, 1983. Microbial utilisation of methanol: production of useful metabolites. Advances in Biotechnological Processes 1: 171–202.
Massey V and Palmer G, 1966. On the existence of spectrally distinct classes of flavoprotein semiquinones. A new method for the quantitative production of flavoprotein semiquinones. Biochemistry 10: 3181–3189.
Mincey T, Tayrien G, Mildvan AS & Abeles RH, 1980. Presence of a flavin semiquinone in methanol oxidase. Proceedings of the National Academy of Science 77: 7099–7101.
Osumi M, Nagano M, Yamada N, Hosoi J and Yanagida M, 1982. Three dimensional structure of the crystalloid in the microbody of Kloeckera sp. Composite crystal model. Journal of Bacteriology 151: 376–383.
Patel RN, Hou CT, Laskin AI and Derelonko P, 1981. Microbial oxidation of methanol: properties of crystallised alcohol oxidase from a yeast, Pichia sp. Archives of Biochemistry and Biophysics 210: 481–488.
Phillips Petroleum Co, 1983. Biochemical conversions by yeast fermentation at high cell densities. US Patent No. 4414329.
Phillips RC, 1984. Lifescan Inc. EEC Patent number 0133481.
Roa M and Blobel G, 1983. Biosynthesis of peroxisomal enzymes in the methylotrophic yeast Hansenula polymorpha. Proceedings of the National Academy of Science 80: 6872–6876.
Roggenkamp R, Janowicz Z, Stanisknowski B and Hollenberg C, 1984. Biosynthesis and regulation of the peroxisomal methanol oxidase from the methylotrophic yeast Hansenula polymorpha. Microbial and General Genetics 194: 489–493.
Sahm H and Wagner F, 1973. Microbial assimilation of methanol. The ethanol- and methanoloxidising enzymes of the yeast Candida boidinii. European Journal of Biochemistry 36: 250–256.
Sakai Y, Sawai T and Tani Y, 1987. Isolation and characterisation of a catabolite repression-insensitive mutant of a methanol yeast, Candida boidinii A5, producing alcohol oxidase in glucose containing medium. Applied and Environmental Microbiology 53: 1812–1818.
Schreiber W, Schindler J and Schmid R, 1975. Henkel and Cie GmbH. German Patent number 2 557 623.
Sherry B and Abeles RH, 1985. Mechanism of action of methanol oxidase, reconstitution of methanol oxidase with 5-Deazoflavin, and inactivation of methanol oxidase by cyclopropanol. Biochemistry 24: 2594–2605.
Unichika Co Ltd, 1986. Alcohol oxidase — a method of manufacturing same. Japanese patent number JP217522.
van der Klei I, Veenhuis M, van der Ley I and Harder W, 1989. Heterologous expression of alcohol oxidase in Saccharomyces cerevisiae: properties of the enzyme and implications for microbody development. FEMS Microbiology Letters 57: 133–138.
Trinder P, 1969. Determination of glucose in blood using glucose oxidase with an alternative oxygen acceptor. Ann Clin Biochem 6: 24–27.
van Dijken JP, Veenhuis M and Harder W, 1982. Peroxisomes of methanol-grown yeasts. Annals of the New York Academy of Science 386: 200–216.
Veenhuis M, van Dijken JP, Pilon SAF and Harder W, 1978. Development of crystalline peroxisomes in methanol-grown cells of the yeast Hansenula polymorpha and its relation to environmental conditions. Archives of Microbiology 117: 153–163.
Veenhuis M, Keizer I and Harder W, 1979. Characterisation of peroxisomes in glucose-grown Hansenula polymorpha and their development after transfer of cells into methanol containing media. Archives of Microbiology 120: 167–175.
Veenhuis M, van Dijken JP and Harder W, 1983. The significance of peroxisomes in the metabolism of one-carbon compounds in yeasts in Advances in Microbial Physiology. Vol. 24 Ed Rose AH, Morris JG and Tempest DW. Academic Press: 2–82.
Veenhuis M, Sulter G, van der Klei and Harder W, 1989. Evidence for functional heterogeneity among microbodies in yeasts. Archives of Microbiology 151: 105–110.
Verduyn C, van Dijken JP and Scheffer WA, 1983. A simple, sensitive and accurate alcohol electrode. Biotechnology and Bioengineering 25: 1049–1056.
Walter P, Gilmore R and Blobel G, 1984. Protein translocation across the endoplasmic reticulum. Cell 38: 5–8.
Waters MG and Blobel G, 1986. Secretory protein translocation in yeast cell-free system can occur post-translationally and requires ATP hydrolysis. Journal of Cell Biology 102: 1543–1550.
Yamada H, Sgin K-C, Kato N, Shimizu S and Tani Y, 1979. Purification and characterisation of alcohol oxidase from Candida 25A. Agricultural and Biological Chemistry 43: 877–878.
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© 1990 Kluwer Academic Publishers
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Woodward, J.R. (1990). Biochemistry and Applications of Alcohol Oxidase from Methylotrophic Yeasts. In: Codd, G.A., Dijkhuizen, L., Tabita, F.R. (eds) Autotrophic Microbiology and One-Carbon Metabolism. Advances in Autotrophic Microbiology and One-Carbon Metabolism, vol 1. Springer, Dordrecht. https://doi.org/10.1007/978-94-009-1978-5_8
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DOI: https://doi.org/10.1007/978-94-009-1978-5_8
Publisher Name: Springer, Dordrecht
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